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KASP-Flw-sau6198 molecular marker linked with wheat flag leaf width major QTL (quantitative trait locus) and application of KASP-Flw-sau6198 molecular marker

A kasp-flw-sau6198, molecular marker technology, applied in the fields of molecular biology and crop genetics and breeding, can solve the problem of not many molecular markers, achieve the effects of accurate and efficient detection, high accuracy, and improve the efficiency of selection and identification

Active Publication Date: 2021-12-21
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many tightly linked molecular markers that are related to the trait of wheat flag leaf width and can be used in practical molecular breeding.

Method used

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  • KASP-Flw-sau6198 molecular marker linked with wheat flag leaf width major QTL (quantitative trait locus) and application of KASP-Flw-sau6198 molecular marker
  • KASP-Flw-sau6198 molecular marker linked with wheat flag leaf width major QTL (quantitative trait locus) and application of KASP-Flw-sau6198 molecular marker
  • KASP-Flw-sau6198 molecular marker linked with wheat flag leaf width major QTL (quantitative trait locus) and application of KASP-Flw-sau6198 molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Obtainment of wheat flag leaf width QTL QFLW.sau-AM-4B.4 and its molecular marker KASP-Flw-sau6198

[0043] (1) Using the wheat line 'Ailanmai' as the female parent and the wheat variety 'LM001' as the male parent to cross, the hybrid F 1 , F 1 F 2 , at F 2 Using the single-ear transmission method, all the way to F 8 Generations, recombinant inbred lines containing 121 lines were obtained to form a genetic mapping population.

[0044] (2) Identification of the flag leaf width phenotype of the recombinant inbred line population: analyze and identify the flag leaf width of the recombinant inbred line at the wheat maturity stage, remove the individual plants at both ends of each row, collect five individual plants with the same growth, and calculate The width of the flag leaf is obtained, and the average value is obtained, which represents the width of the flag leaf of the strain.

[0045] (3) 55K SNP chip analysis

[0046] a) DNA extraction: The DNA of the...

Embodiment 2

[0058] Example 2 Application of Molecular Marker KASP-Flw-sau6198 in Selection and Control of Flag Leaf Width QTL QFLW.sau-AM-4B.4

[0059] (1) Using the tetraploid wheat 'PI 503554' with narrower flag leaves as the male parent and the tetraploid local wheat 'Ailanmai' with wider flag leaves as the female parent to construct a recombinant inbred line, and randomly select the offspring lines 54 strains.

[0060] (2) Carry out KASP-Flw-sau6198 marker detection on the obtained 54 strains, the specific method is: extract the DNA of 54 strains; use it as a template, and use the specific primer pair of molecular marker KASP-Flw-sau6198 Perform PCR amplification and perform fluorescence readings for primers, which are:

[0061] Primers on the FAM tag: (the underlined part is the FAM tag sequence) 5'- GAAGGTGACCAAGTTCATGCT TCGACAGCCGGACTTCTCGA-3' (SEQ ID No. 1)

[0062] Primers on the HEX tag: (the wavy part is the HEX tag sequence) 5'-

[0063]

[0064] Universal downstream p...

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Abstract

The invention discloses a KASP-Flw-sau6198 molecular marker linked with a wheat flag leaf width major QTL (quantitative trait Locus) and application of the molecular marker, and belongs to the fields of molecular biology and crop genetic breeding. The molecular marker is an SNP molecular marker, the polymorphism is A / G, the molecular marker and wheat flag leaf width QTL QFLW.sau-AM-4B. 4 are co-located on a long arm of a tetraploid wheat 4B chromosome, and the molecular marker is located in the QTL QFLW.sau-AM-4B. 4 interval. Detection and analysis show that the molecular marker can accurately track the wheat flag leaf width QTLQFLW.sau-AM-4B. 4 and predict the flag leaf width characteristic of wheat, wheat varieties or strains with wide flag leaves can be more rapidly screened out for breeding, and the wheat breeding process is greatly accelerated.

Description

technical field [0001] The invention relates to the field of molecular biology and crop genetic breeding, in particular to KASP-Flw-sau6198 molecular marker linked with wheat flag leaf width major QTL and application thereof. Background technique [0002] Wheat (Triticum sp.) is one of the most widely planted crops in the world. Facing the ever-increasing world population, how to increase wheat yield has become a key issue for breeders. [0003] The flag leaf is the main photosynthetic organ of wheat, which affects the accumulation of dry matter and the formation of yield. Therefore, the size of the flag leaf has a great influence on the final yield of wheat. Flag leaf width is an important agronomic trait. It is not only one of the important components of wheat plant type, but also the main organ of plant photosynthesis. It plays an important role in yield potential in wheat. During the filling stage, half of the organic matter in the seeds is provided by the flag leaves,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6851C12N15/11
CPCC12Q1/6895C12Q1/6851C12Q2600/13C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 马建王健赵聪豪王素容曾照勇刘航唐华苹牟杨邓梅苟璐璐谭翠江千涛魏育明郑有良兰秀锦
Owner SICHUAN AGRI UNIV
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