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Method for improving accuracy of protein content modeling basic data in near infrared spectrum analysis technology

An accuracy and data technology, which is applied in the direction of material analysis, material analysis, and measuring devices through optical means, can solve problems such as greater impact on accuracy, and achieve the effect of improving data detection accuracy and fast detection speed

Active Publication Date: 2019-02-12
SHANDONG UNIV
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Problems solved by technology

At the same time, NIRS also has certain limitations, mainly reflected in the fact that NIRS is a secondary analysis method, which requires traditional analysis methods to measure basic data, and its accuracy is greatly affected by the reference method

Method used

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  • Method for improving accuracy of protein content modeling basic data in near infrared spectrum analysis technology
  • Method for improving accuracy of protein content modeling basic data in near infrared spectrum analysis technology
  • Method for improving accuracy of protein content modeling basic data in near infrared spectrum analysis technology

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Embodiment 1

[0031] 1. Error analysis of human plasma reference substance measured by biuret method and Kjeldahl method

[0032] Raw material human plasma reference material (Shandong Taibang Biological Products Co., Ltd., China, protein content value = 58.1), using the Kjeldahl method and the biuret method (AU5800 automatic biochemical analyzer, Beckman, the United States) in parallel Measure 20 times and calculate the average value of the measurement error. Utilize Kjeldahl method and biuret method to measure the result of raw material human plasma reference substance as follows figure 1 shown. The average measurement errors of the biuret method and the Kjeldahl method are 1.676g / L and 0.672g / L respectively, that is, the accuracy of the Kjeldahl method is better than that of the biuret method. Therefore, it is proposed that for the biuret method with larger measurement errors, if you want to obtain a stable and accurate cumulative average value, you should increase the measurement data...

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Abstract

The invention belongs to the field of protein content detection, and particularly relates to a method for improving the accuracy of basic data, i.e., protein detection data, in a near infrared spectrum analysis technology on the basis of the thought "data equalization". In the prior art, a method for three-time measurement for averaging is generally adopted by aiming a protein content detection problem to improve data accuracy. The thought "data equalization" can be applied to a protein content detection problem, an optimal measurement frequency for protein content measurement is obtained, andmeasurement is carried out for multiple times for averaging to improve the accuracy of protein detection data. The method is applied to the basic data detection of human plasma protein modeling in the near infrared spectrum analysis technology, and the parallel measurement frequency of the basic data should be 39 times. The RMSEP (Root-Mean-Square Error of Prediction) of the near infrared spectrum analysis model established with the method is improved than the RMSEP of the repetition of parallel measurement for three times for averaging by 23.28%. The method is applied to plasma protein detection and has an important meaning.

Description

technical field [0001] The invention belongs to the field of protein content detection, and specifically relates to a method for improving the accuracy of basic data in near-infrared spectroscopy analysis technology, that is, protein detection data, based on the idea of ​​"data averaging". Background technique [0002] Biuret (NH 2 CONH CONH 2 ) is the product obtained after two molecules of urea are heated at about 180°C to release one molecule of ammonia. Any compound with two amide groups or two directly connected peptide bonds, or a peptide bond that can be connected by an intermediate carbon atom, can undergo biuret reaction. In strong alkaline solution, biuret forms a purple complex with divalent copper ions. The color of the purple complex is proportional to the protein concentration and has nothing to do with the protein molecular weight and amino acid composition, so it can be used to determine the protein content. The biuret method has similar color reactions to...

Claims

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Application Information

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IPC IPC(8): G01N21/359
CPCG01N21/359
Inventor 臧恒昌孙钟毓聂磊李丹阳井金荣杜冉冉刘瑞琛于宸
Owner SHANDONG UNIV
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