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Method for establishing hybrid line of Japanese white crucian carp and red crucian carp and cultivation method of zygotic crucian carp No. 2

A technique for establishing and cultivating methods is applied to the cultivation of Hefang crucian carp No. 2 and the establishment of hybrid strains of Japanese white crucian carp and red crucian carp. Strong resistance to stress and disease, the effect of sweet meat

Active Publication Date: 2019-02-15
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Japanese white crucian carp grows fast, the meat quality is not good; although the red crucian carp is sweet and tender, its growth rate is not as good as that of the Japanese white crucian carp.

Method used

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  • Method for establishing hybrid line of Japanese white crucian carp and red crucian carp and cultivation method of zygotic crucian carp No. 2
  • Method for establishing hybrid line of Japanese white crucian carp and red crucian carp and cultivation method of zygotic crucian carp No. 2
  • Method for establishing hybrid line of Japanese white crucian carp and red crucian carp and cultivation method of zygotic crucian carp No. 2

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Embodiment 1

[0045] A method for establishing a hybrid strain of Japanese white crucian carp and red crucian carp of the present invention, comprising the steps of:

[0046] 1. Hefang crucian carp F 1 cultivation

[0047] Mix the sperm of bream inactivated by ultraviolet rays with the mature eggs of Japanese white crucian carp / red crucian carp, and after 3 to 4 minutes, place the activated eggs at 3 to 4°C for 35 to 45 minutes of cold shock treatment to obtain After hatching the embryos at a water temperature of 22-23°C, a diploid gynogenetic female Japanese white crucian carp / male red crucian carp was selected. , Fertility testing, screening to obtain diploid combined square crucian carp F 1 (See figure 1 A).

[0048] Kidney cell direct preparation method for Hefang crucian carp F 1 Sampling and detection of somatic cell chromosome number (namely kidney tissue chromosomal ploidy detection method), it was found that Hefang crucian carp F 1 All are diploid (2n=100), the chromosome map...

Embodiment 2

[0075] A kind of cultivation method of Hefang crucian carp No. 2 of the present invention, comprises the steps:

[0076] (1) Selection and cultivation of broodstock: During January-February of that year, pull nets to catch fish, select from the above-mentioned embodiment 1 more than 600g of body weight, obvious sexual maturity characteristics, no disease and no injury, good body shape, bright body color and physical fitness Robust and sexually fertile diploid female zygote crucian carp F 1 and the male Japanese white crucian carp as the female parent fish and the male parent fish respectively for special pond cultivation. During the special pond cultivation, 80-100m 2 Install 1-2 oxygenation pumps in the cement pool, and install 2-4 submersible pumps on the inner wall of the cement pool as the driving force for water stimulation. The oxygenation pumps are turned on regularly, and the feed is fed regularly and quantitatively. The female Hefang crucian carp in the pool f 1 The...

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Abstract

The invention discloses a method for establishing a hybrid line of Japanese white crucian carp and red crucian carp, wherein a female Japanese white crucian carp and male red crucian carp obtained byartificial induction of gynogenesis are raised as a female parent and a male parent respectively. The offspring obtained from hybrid are raised and screened to obtain diploid zygotic crucian carp F1 which is amphoteric fertile; the diploid zygotic crucian carp F1 is cultivated and is subjected to self-cross to obtain zygotic crucian carp F2 which is amphoteric fertile, amphoteric fertile diploid zygotic curucian carp Fn is obtained through self-cross at least twice. A diploid hyprid crucian carp line which is stable in inheritable characters and amphoteric fertile is obtained. The line has important significance in fish genetic breeding and biological evolution and provides high-quality germplasm resources for breeding novel fine crucian carp. The invention also discloses a cultivation method for the zygotic crucian carp No. 2, which is obtained by backcrossing breeding male Japanese white crucian carp F1 with female parent zygotic crucian carp No. 1. The method is simple and easy to operate. The zygotic crucian carp No.2 has high dorsum, grows fast, has sweet and tender meat, and is strong resistance to stress and diseases.

Description

technical field [0001] The invention belongs to the field of fish hybridization, and in particular relates to a method for establishing a hybrid strain of Japanese white crucian carp and red crucian carp and a method for cultivating Hefang crucian carp No. 2. Background technique [0002] Japanese white crucian carp (Carassius auratus cuvieri) belongs to the family Cyprinidae, belongs to the genus Carassius, and the carassius species (Carassius auratus) is a diploid fish (2n=100). Japanese white crucian carp was formerly known as Yuanwulang crucian carp or Hanoi crucian carp, Dagu crucian carp, Osaka crucian carp, and Japanese crucian carp. Originally produced in Lake Biwa, Japan, it was introduced to my country in 1976. It is named "white crucian carp" because of its silvery-white body color. The Japanese white crucian carp is large in size, the largest individual can reach five catties, its body is tall and flat on the side, its back has a prominent bulge, like a hunchbac...

Claims

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Application Information

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IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 刘少军刘庆峰梁秋丽刘俊梅陶敏张纯覃钦博王静罗凯坤
Owner HUNAN NORMAL UNIVERSITY
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