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Preparation method of salmonella anatis attenuated vaccine

A Salmonella and attenuated vaccine technology, which is applied in the field of preparation of duck Salmonella attenuated vaccine, can solve problems such as difficulties in the prevention and control of salmonellosis, and achieve the effects of reducing breeding costs, good clinical effects, and highly targeted immunogenicity

Inactive Publication Date: 2019-02-15
ZHENGZHOU HOUYI PHARMA
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Problems solved by technology

[0002] Salmonella is a member of the Enterobacteriaceae family, with more than 2,500 serotypes, widely distributed in nature, and almost all animals can be infected. Because of the large number of serotypes, the infection spectrum of different animals is different, which brings great challenges to the prevention and control of salmonellosis. great difficulty

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  • Preparation method of salmonella anatis attenuated vaccine

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preparation example Construction

[0022] A kind of preparation method of duck Salmonella attenuated vaccine comprises the following steps,

[0023] a. Select diseased ducks infected with Salmonella from duck farms susceptible to Salmonella infection, and isolate the original strain;

[0024] b. Mutagenize the original strains by ultraviolet and nitrosoguanidine respectively to obtain their respective optimal mutagenesis conditions. Under the optimal mutagenesis conditions, the bacterial mortality rate is about 80-90%, and then carry out ultraviolet-ray- Compound mutagenesis with nitrosoguanidine to obtain a weakened mutagenized strain;

[0025] c. Expanding and culturing the weakened mutagenized strain to prepare an attenuated Salmonella vaccine.

[0026] The specific steps of said step b are,

[0027] (1) Take the diseased duck infected with Salmonella, dissect the diseased tissue, inoculate it with common LB solid medium, and cultivate it in a 37°C incubator for 18-24 hours. Pick out the round edge of the ...

Embodiment 1

[0035] A kind of preparation method of duck Salmonella attenuated vaccine comprises the following steps,

[0036] a. Select diseased ducks infected with Salmonella from duck farms susceptible to Salmonella, dissect the diseased tissues, and isolate the original strain;

[0037] b. Inoculate ordinary LB solid medium, culture in a 37°C incubator for 18 hours, pick the circular colonies with neat edges, smooth surface, translucent, and small protrusions grown on the LB solid medium, and inoculate them in the color-developing culture of Salmonella Base and DHL Salmonella identification medium, select the strains that show lavender on the Salmonella chromogenic medium and black on the DHL Salmonella identification medium at the same time, after passage and purification, expand the culture, and prepare 1× with normal saline after counting the bacteria. 10 8 The original bacterial suspension of CFU / ml, the original bacterial suspension was stirred and irradiated for 10s by ultravio...

Embodiment 2

[0040] A kind of preparation method of duck Salmonella attenuated vaccine comprises the following steps,

[0041] a. Select diseased ducks infected with Salmonella from duck farms susceptible to Salmonella, dissect the diseased tissues, and isolate the original strain;

[0042] b. Inoculate ordinary LB solid medium, culture in a 37°C incubator for 24 hours, pick out the circular colonies with neat edges, smooth surface, translucent, and small protrusions grown on LB solid medium, and inoculate them in the chromogenic culture of Salmonella respectively Base and DHL Salmonella identification medium, select the strains that show lavender on the Salmonella chromogenic medium and black on the DHL Salmonella identification medium, after passage and purification, expand the culture, and prepare 5× with normal saline after counting the bacteria. 10 8 The original bacterial suspension of CFU / ml, the original bacterial suspension was stirred and irradiated for 30 seconds by ultraviole...

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Abstract

The invention discloses a preparation method of a salmonella anatis attenuated vaccine. The method comprises the following steps of: a, selecting a diseased duck infected with salmonella from a duck farm susceptible to salmonella, and isolating an original strain SC26; b, respectively carrying out ultraviolet and nitrosoguanidine mutagenesis on original strain SC26 to obtain respective optimal mutagenesis conditions, under which the bacterial mortality rate is about 80-90%, and then carrying out ultraviolet-nitrosoguanidine compound mutagenesis under the respective optimal mutagenesis conditions to obtain a weakened mutagenized strain SC2261; and c, the carrying out enlarge cultivation on a weakened mutagenized strain SC261 to prepare a salmonella attenuated vaccine. By taking local strains of disease areas, the preparation method of the salmonella anatis attenuated vaccine is strong in immunogenicity pertinence and good in clinical effect, and is equivalent to a special vaccine.

Description

technical field [0001] The invention belongs to the field of animal bacterial attenuated vaccines, and in particular relates to a preparation method of duck Salmonella attenuated vaccines. Background technique [0002] Salmonella is a member of the Enterobacteriaceae family, with more than 2,500 serotypes, widely distributed in nature, and almost all animals can be infected. Because of the large number of serotypes, the infection spectrum of different animals is different, which brings great challenges to the prevention and control of salmonellosis. Big difficulty. [0003] To successfully get rid of the problem of Salmonella, it is necessary to implement prevention and control from all links in the entire production chain, that is, from the farm to the dining table, which mainly involves the three links of the feed industry, farms, and slaughtering and processing, so as to reduce the infection rate of Salmonella in livestock and poultry It is considered to be an important ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/112A61P31/04
CPCA61K39/0275A61K2039/522A61K2039/552A61P31/04Y02A50/30
Inventor 魏志刚刘应鹏
Owner ZHENGZHOU HOUYI PHARMA
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