ECHO virus for treating tumors

A virus and tumor technology, applied in the field of virus and tumor treatment

Active Publication Date: 2019-03-05
XIAMEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports of echovirus type 25 having oncolytic activity in this field

Method used

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  • ECHO virus for treating tumors
  • ECHO virus for treating tumors
  • ECHO virus for treating tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0269] Example 1: Acquisition and preparation of ECHO25 and its modified forms

[0270] 1.1 Isolation of ECHO25 from clinical samples of patients

[0271] (1) The patients' throat swabs and anal swabs are from China, Xiamen Center for Disease Control and Prevention; African green monkey kidney cells (Vero cells; Number: CCL-81 TM) is preserved by China, Xiamen University, National Engineering Technology Research Center for Diagnostic Reagents and Vaccines of Infectious Diseases, and cultured in MEM medium supplemented with 10% fetal bovine serum, glutamine, penicillin and streptomycin.

[0272] (2) Specimen processing: Fully stir the patient's throat swab and anal swab in the specimen preservation solution to wash off the virus and virus-containing cells attached to the swab, and then store the specimen preservation solution at 4°C 4000rpm high-speed centrifugation for 30min;

[0273] (3) Inoculation and observation:

[0274] A. Spread Vero cells on a 24-well plate, and...

Embodiment 2

[0291] Example 2: In vitro anti-tumor experiments of ECHO25 and its modified forms

[0292] 2.1 Viruses and cell lines used

[0293] (1) Viruses: In this example, ECHO25-WT (SEQ ID NO: 12), ECHO25-HRV2 (SEQ ID NO: 13), ECHO25-miR133&206T (SEQ ID NO: 14), ECHO25- GM-CSF (SEQ ID NO: 15) and ECHO25-Anti-PD-1 (SEQ ID NO: 16).

[0294] (2) Cell line: human rhabdomyosarcoma cell RD ( Number: CCL-136 TM ); human colorectal cancer cell line SW480 ( Number: CCL-228 TM ) and HT-29 ( Number:HTB-38 TM ); human gastric cancer cell line AGS ( Number: CRL-1739 TM ), SGC7901 (CCTCC deposit number: GDC150), BGC823 (CCTCC deposit number: GDC151) and NCI-N87 ( Number: CRL-5822 TM ); human small cell lung cancer cell line NCI-H1417 ( Number: CRL-5869 TM ); human non-small cell lung cancer cell line SPC-A-1 (CCTCC deposit number: GDC050), NCI-H1299 ( Number: CRL-5803 TM ), NCI-H1975 ( Number: CRL-5908 TM ), A549 ( Number: CCL-185 TM ), NCI-H661 ( Number:HTB-183 TM )...

Embodiment 3

[0337] Example 3: In vivo anti-tumor experiments of ECHO25 and its modified forms

[0338] 3.1 Viruses, cell lines and experimental animals

[0339] (1) Viruses: ECHO25-WT (SEQ ID NO: 12), ECHO25-HRV2 (SEQ ID NO: 13), ECHO25-miR133&206T (SEQ ID NO: 14), ECHO25 provided in Example 1 were used in this example - GM-CSF (SEQ ID NO: 15) and ECHO25-Anti-PD-1 (SEQ ID NO: 16). Refer to Examples 2.2 and 2.3 for the methods of virus cultivation and virus titer determination, respectively.

[0340] (2) Cell lines: human glioma cell line GBM (primary tumor cell line isolated from patient tumor tissue), human endometrial cancer cell line Ishikawa (ECACC No.99040201), human prostate cancer cell line PC- 3( Number: CRL-1435 TM ) and human breast cancer cell line BcaP37 (CCTCC deposit number: GDC206). The above cells were all cultured in DMEM medium, and the medium was added with 10% fetal bovine serum, glutamine and penicillin-streptomycin double antibody. All above cells were store...

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PUM

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Abstract

The invention relates to the field of virus and the field of tumor treatment. Concretely, the invention relates to ECHO 25 or a modified form thereof, or a genomic sequence or cDNA sequence comprisingthe ECHO 25 or the modified form of the ECHO 25, or nucleic acid molecules of a complementary sequences of the genomic sequence or cDNA sequence, applications in treating tumors for a subject such asa human being, and applications in preparing pharmaceutical compositions for treating the tumors for the subject such as a human being. The invention also relates to a method for treating the tumors.The method includes steps of applying the ECHO 25 or the modified form of the ECHO 25, or the genomic sequence or cDNA sequence comprising the ECHO 25 or the modified form of the ECHO 25 or the nucleic acid molecules of the complementary sequences of the genomic sequence or cDNA sequence to the subject who needs the treatment.

Description

technical field [0001] The invention relates to the field of viruses and the field of tumor treatment. Specifically, the present invention relates to echovirus type 25 (ECHO25) or a modified form thereof, or a nucleic acid molecule comprising the genome sequence or cDNA sequence of ECHO25 or a modified form thereof, or a complementary sequence of the genome sequence or cDNA sequence, used Use for treating a tumor in a subject (eg, a human), and use in the manufacture of a medicament for treating a tumor in a subject (eg, a human). The present invention also relates to a method for treating tumors, which comprises administering ECHO25 or a modified form thereof, or a genomic sequence or cDNA sequence comprising ECHO25 or a modified form thereof, or the genomic sequence or cDNA sequence to a subject in need thereof The steps of the complementary sequence of nucleic acid molecules. Background technique [0002] The current treatment methods for malignant tumors mainly include...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/768A61K45/06A61K48/00A61P35/00C12N7/01C12N15/41C12R1/93
CPCA61K45/06A61K48/0008A61K35/768C12N7/00C12N2770/32021A61K2300/00A61K31/711A61P35/00A61K31/407A61K31/519A61K31/675A61K31/704A61K31/7048A61K31/555A61K31/513C12N2770/32332C12N2770/32321C12N2770/32322A01K2207/12A01K2227/105A01K2267/0331A61K35/76A61K48/00C12N2770/32333A61K35/66C07K14/00C12N15/86C07K14/005C12N15/79A61K45/00C12N15/63C12N15/09A61K38/00A61K35/00A61K38/16
Inventor 程通王玮万俊凯徐龙发叶祥忠张军夏宁邵
Owner XIAMEN UNIV
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