A kind of natural immune booster for aquatic products and its application

A technology of natural immunity and enhancer, applied in applications, biochemical equipment and methods, animal husbandry, etc., can solve problems such as poor immune enhancement effect, improve immunity and anti-stress ability, improve survival rate, improve The effect of the body's immunity

Active Publication Date: 2022-05-17
浙江皇冠科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immune enhancement effect of brewer's yeast extract in the immune enhancer is not good, and the immune enhancer cannot make aquatic products have broad-spectrum resistance

Method used

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  • A kind of natural immune booster for aquatic products and its application
  • A kind of natural immune booster for aquatic products and its application
  • A kind of natural immune booster for aquatic products and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A natural immune enhancer for aquatic products, the natural immune enhancer includes CpG oligonucleotides, the preparation method of the above CpG oligonucleotides is: adding STET buffer solution to the yeast cell, under the condition of 1200r / min Stir magnetically for 20 minutes, then add freshly prepared lysozyme to a final concentration of 120 μg / mL, stir evenly and place in a water bath at 35°C for 30 minutes, then add cell lysate and heat in a boiling water bath for 10 minutes, then rapidly cool to room temperature, then Centrifuge at 15000r / min for 20min, then add the supernatant to 0.8 times the volume of pre-cooled isopropanol, mix well and settle at -6°C for 25min, then centrifuge at 10000r / min After 20 minutes, discard the supernatant, dissolve the precipitate with sterile water, and freeze-dry to obtain the CpG oligonucleotide. Calculate the actual content of the CpG oligonucleotide. The CpG oligonucleotide is unmethylated and contains a motif. Oligonucleotid...

Embodiment 2

[0040] A natural immune enhancer for aquatic products, comprising the following components and their parts by weight: 10 parts of CpG oligonucleotides, 5 parts of Phaffia powder rich in astaxanthin, 20 parts of fructooligosaccharides, and 10 parts of mannooligosaccharides .

[0041] A preparation method of a natural immune enhancer for aquatic products, the concrete steps are:

[0042] 1) Strain activation: select Saccharomyces cerevisiae containing highly immunoreactive CpG oligonucleotides (pYES2-90CpG plasmid), inoculate engineered Saccharomyces cerevisiae strains on SC-U slant medium, and culture them at 30°C for 45 hours. spare;

[0043] 2) Shake flask culture: inoculate the activated slant strains into the YEPD seed medium, and cultivate for 24 hours at a temperature of 30°C and a rotation speed of 150r / min to obtain a first-class seed culture, and then culture the first-class seed The inoculum was inoculated into the fermentation medium at an inoculum size of 8% (V / V)...

Embodiment 3

[0049] A natural immune enhancer for aquatic products, comprising the following components and their parts by weight: 9 parts of CpG oligonucleotides, 8 parts of Phaffia powder rich in astaxanthin, 15 parts of fructooligosaccharides, and 15 parts of mannooligosaccharides .

[0050] A preparation method of a natural immune enhancer for aquatic products, the concrete steps are:

[0051] 1) Strain activation: select Saccharomyces cerevisiae containing highly immunoreactive CpG oligonucleotides (pYES2-90CpG plasmid), inoculate engineered Saccharomyces cerevisiae strains on SC-U slant medium, and culture them at 28°C for 48 hours. spare;

[0052] 2) Shake flask culture: inoculate the activated slant strains into the YEPD seed medium, cultivate for 20 hours at a temperature of 28°C and a rotation speed of 200r / min to obtain a first-grade seed culture, and then culture the first-grade seed The inoculum is inoculated into the fermentation medium at an inoculation amount of 10% (V / V)...

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PUM

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Abstract

The invention discloses a natural immune enhancer for aquatic products and its application. The natural immune enhancer comprises the following components and parts by weight: 8-10 parts of CpG oligonucleotides, 5 parts of Phaffia yeast powder rich in astaxanthin ‑10 parts, fructooligosaccharides 10‑20 parts, mannan oligosaccharides 10‑20 parts. The natural immune enhancer is applied to the feed of aquatic animals, and its added amount is 0.05-0.3% of the weight of the feed. The beneficial effects are: each component in the natural immune enhancer of the present invention plays a role of mutual gain, improves the immunity and anti-stress ability of aquatic animals, improves the resistance to diseases, promotes the growth of aquatic animals, and has low cost; the natural immune The enhancer is used in the feed of aquatic animals to improve the survival rate of the whole cycle of aquatic animals and make up for the problem of adding antibiotics in the feed.

Description

technical field [0001] The invention relates to the technical field of feed additives, in particular to a natural immune enhancer for aquatic products and its application. Background technique [0002] The destruction of the natural ecological environment and the savage plundering of humans have greatly reduced the number and scale of aquatic animals, and the natural resources of aquatic products have been severely damaged. Some species even cannot form natural populations or are on the verge of extinction. In order to protect natural resources and meet human needs, my country has vigorously developed aquaculture. More and more aquaculture species have formed large-scale farming. When aquaculture is sick or threatened by surrounding epidemics, fishermen often use drugs to prevent and control diseases in order to reduce further economic losses. When choosing drugs, most fishermen choose chemical drugs, especially the environmental disinfection drugs and antibiotic drugs, wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/117C12N15/10A23K20/153
CPCA23K20/153C12N15/117C12N2330/10C12N2310/17
Inventor 胡向东项黎新叶茂
Owner 浙江皇冠科技有限公司
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