Application of mangostin in prevention of bacterial wilt

A technology of mangostin and bacterial wilt, which is applied in the application field of mangostin in the prevention and treatment of bacterial wilt, can solve the problems of no mangostin, etc., and achieve a good inhibitory effect

Active Publication Date: 2019-03-15
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But, so far, there is no report that mangostin is used in th

Method used

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  • Application of mangostin in prevention of bacterial wilt
  • Application of mangostin in prevention of bacterial wilt
  • Application of mangostin in prevention of bacterial wilt

Examples

Experimental program
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Embodiment 1

[0030] 1. Effects of γ-mangosteen and α-mangosteen on the growth curve of R. solanacearum

[0031] (1) Prepare γ-mangosteen (γ-mangostin) and α-mangosteen (α-mangostin) respectively into 200 μg / mL mother liquor, and filter for later use (diameter 13mm, pore size 0.22um);

[0032] (2) configure NB culture medium (i.e. NB culture medium), autoclave for standby;

[0033] (3) Use a pipette gun to draw γ-mangosteen and α-mangosteen spare mother solutions into 30 mL NB culture solution respectively in the aseptic operation table, so that the final concentrations of the drug-containing culture solution are 10 μg / mL, 25 μg / mL, and 50 μg, respectively / mL, 100 μg / mL, 200 μg / mL, with a final concentration of 0.5% (v / v) DMSO (dimethyl sulfoxide) solvent control (CK), set three repetitions;

[0034] (4) Inoculate 100uL (OD value = 1.0) of the spare bacterial solution of R. solanacearum (R. solanacearum) into 30mL of medicated culture solution, and culture it in a shaking table at 30°C an...

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Abstract

The invention discloses application of mangostin in prevention of bacterial wilt. The invention discloses that gamma-mangostin and alpha-mangostin have a good inhibition effect on Ralstonia solanacearum, and also discovers that after the Rastonia solanacearum is treated by the gamma-mangostin or the alpha-mangostin, the formation amount of Ralstonia solanacearum biological membranes is reduced, the length of a cell is obviously increased, the cell is deformed, a cell membrane is shrunk to different degrees, and the cell morphology is damaged, so that the growth and reproduction of the Rastoniasolanacearum are inhibited. Therefore, the gamma-mangostin and the alpha-mangostin can serve as an inhibitor or a pesticide preparation of the Rastonia solanacearum for preventing and treating agriculture-related diseases such as the bacterial wilt and the like, or the gamma-mangostin and the alpha-mangostin can serve as a soil additive and are added into soil for preventing and treating the bacterial wilt.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to the application of mangostin in the prevention and treatment of bacterial wilt. Background technique [0002] Ralstonia solanacearum is a soil-borne pathogenic bacterium that can cause plant bacterial wilt. Ralstonia solanacearum has become one of the most destructive plant bacterial diseases due to its extremely strong infectivity, global geographical distribution and unusually wide host range. Once the disease occurs, it can cause plant stems and leaves to wilt and die , so far there is no effective control method, known as the "cancer" of plants. Ralstonia solanacear can harm a variety of plants, and it has been reported in recent years that it can infect more than 200 species of plants in 53 families. Ralstonia solanacearum is another complex strain with high variability and adaptability, and it has many physiological and biochemical pathogenic pat...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P1/00
CPCA01N43/16
Inventor 颜健黎平
Owner SOUTH CHINA AGRI UNIV
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