A kind of preparation method of anti-aging fish-derived collagen peptide

A collagen peptide and anti-aging technology, which is applied in the field of preparation of fish-derived collagen peptide, can solve problems such as unobtained, and achieve the effects of simple process, improved biological titer, and no environmental pollution.

Active Publication Date: 2021-08-03
SHANDONG SHENGHAI HEALTH PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional Chinese medicine believes that fish maw collagen products have various physiological effects such as improving qi and blood, tonifying kidney and strengthening essence, nourishing skin and moisturizing skin, nourishing tendons, improving immunity, strengthening brain and invigorating intelligence, protecting against cold and dampness, stopping bleeding and removing blood stasis, promoting lactation and reducing swelling, etc. But almost none of them have been confirmed by modern scientific research

Method used

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  • A kind of preparation method of anti-aging fish-derived collagen peptide
  • A kind of preparation method of anti-aging fish-derived collagen peptide
  • A kind of preparation method of anti-aging fish-derived collagen peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Thaw 10kg of sturgeon swim bladder, wash, drain, cut into pieces (3×3cm), crush and pass through a 5-mesh sieve to remove impurities; then, add 50L of industrial alcohol, and degrease with 50W / L ultrasonic wave at 30°C for 3 hours; press After filtering and crushing, add 30L No. 6 solvent oil, degrease with 10W / L ultrasonic wave at 50°C for 5 hours; then press filter to obtain about 3.0kg of degreased sturgeon swim bladder;

[0039]Add 30L of hot water at 50°C to the above-mentioned defatted fish maw, and extract collagen for 1 hour under stirring at 200rpm; then pass through a 20-mesh sieve, cool to 35°C, add 0.3L of concentrated hydrochloric acid and pepsin to the filtrate to 2500U / mL, Stir continuously at 150rpm for 3h to simulate gastric phase digestion; then adjust the pH to 6.8 with NaOH, add trypsin to 20000U / mL, stir continuously at 200rpm for 6h to simulate intestinal phase digestion; finally boil for 10min, cool, and centrifuge at 10000g for 20min, the obtained...

Embodiment 2

[0042] Thaw 10kg cod swim bladder, wash, drain, cut into pieces (about 1×1cm), crush and pass through a 10-mesh sieve to remove impurities; add 30L industrial alcohol, and degrease with 10W / L ultrasonic wave at 50°C for 5 hours; press filter After crushing, add 50L No. 6 solvent oil, and degrease with 50W / L ultrasonic wave at 30°C for 3 hours; finally press filter to obtain about 3.5kg of degreased cod swim bladder;

[0043] Add 25L of hot water at 60°C to the above-mentioned defatted fish maw, and extract collagen for 2 hours while stirring at 300rpm; Stir continuously for 2 hours to simulate gastric phase digestion; then use NaOH to adjust the pH to 7.4, add trypsin to 30000U / mL, stir continuously at 150rpm for 4 hours to simulate intestinal phase digestion; finally boil for 15 minutes, cool, and centrifuge at 5000g for 30 minutes, the obtained supernatant is Collagen hydrolyzate;

[0044] Using an ultrafiltration membrane with a molecular weight cut-off of 1000Da, the coll...

Embodiment 3

[0046] Thaw 10kg catfish swim bladder, wash, drain, cut into pieces (about 2×2cm), crush and pass through a 5-mesh sieve to remove impurities; add 40L industrial alcohol, degrease with 30W / L ultrasonic wave at 40°C for 4 hours; press filter After crushing, add 40L of No. 6 solvent oil, and degrease with 30W / L ultrasonic wave at 40°C for 4 hours; finally press filter to obtain about 3.2kg of degreased catfish swim bladder;

[0047] Add 27.5L of hot water at 55°C to the above-mentioned pretreated swim bladder, extract collagen for 1.5h under stirring at 250rpm; then pass through a 30-mesh sieve, cool to 37°C, add concentrated hydrochloric acid 0.2L and pepsin to 4000U to the filtrate / mL, continuously stirred at 175rpm for 2.5h to simulate gastric phase digestion; then adjusted the pH to 7.0 with NaOH, added trypsin to 25000U / mL, continuously stirred at 175rpm for 5h to simulate intestinal phase digestion; finally boiled for 12min, cooled, and centrifuged at 7500g After 25 minut...

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Abstract

The invention belongs to the field of biotechnology, and relates to a method for preparing a fish-derived collagen peptide that delays aging; the specific steps are: firstly, the swim bladder is thawed, cleaned, drained, cut into pieces, crushed, and impurities are removed, and industrial alcohol is added for ultrasonication Auxiliary degreasing; press filtration and crushing, then add No. 6 solvent oil for ultrasonic assisted degreasing; then press filtration, add hot water to extract collagen, filter to get the filtrate; after cooling, add concentrated hydrochloric acid and pepsin to simulate gastric phase digestion; adjust After pH, add trypsin to simulate intestinal digestion; finally boil, cool, and centrifuge, and ultrafilter the obtained supernatant, then nanofilter the filtrate again, collect the retentate and spray-dry it to obtain fish-derived collagen peptides ; The present invention realizes the high-profit utilization of fish processing waste, and the prepared collagen peptide has the effect of delaying aging; the production process is simple, the cycle is short, the cost is low, and there is no environmental pollution. The collagen peptide can be applied to food, medicine, Cosmetics and other fields.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing fish-derived collagen peptides for delaying aging. [0002] technical background [0003] Collagen products have been widely used in food, medicine, cosmetics, biological materials and other fields. Traditionally, collagen is mainly derived from livestock and poultry, but due to the frequent occurrence of zoonotic diseases between humans and livestock (foot-and-mouth disease, mad cow disease, Streptococcus suis, avian influenza), its safety is becoming more and more worrying; at the same time, religious customs also Its application and trade are subject to certain restrictions. Our country is a large fishery country, and a large amount of processing waste every year has caused serious waste of resources and environmental pollution. Therefore, using aquatic product processing waste as raw material to produce collagen products that can replace tradit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06
Inventor 王林高瑞昌袁丽周越孙全才
Owner SHANDONG SHENGHAI HEALTH PROD CO LTD
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