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Tissue culture method of spathiphyllum kochii petioles

A technique of tissue culture and petiole, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of unrecorded petiole culture technology of white crane lily, and achieve the effects of low production cost, cost saving and good growth

Active Publication Date: 2019-03-29
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no record of the cultivation technique for the petiole of the white crane lily

Method used

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  • Tissue culture method of spathiphyllum kochii petioles
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  • Tissue culture method of spathiphyllum kochii petioles

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Embodiment 1

[0015] Embodiment 1: a kind of method for culturing the petiole tissue of Heliconia chinensis, the method comprises the following steps:

[0016] (1) From 7:00 a.m. to 9:00 a.m., a mother plant with strong growth, emerald green leaves and no pests and diseases is collected, and the mother plant is the young petiole of the white crane;

[0017] (2) clean up the impurities on the petiole with cotton balls, and clean and disinfect the petiole;

[0018] (3) Cut the sterilized petiole into about 1 cm long segment with a blade, then cut a small knife at 1 / 2 of the stem segment to increase the surface area of ​​the wound to facilitate callus induction, and inoculate the petiole into MS medium In this MS medium, add 6-BA, NAA, agar 6g / L and sucrose 30g / L, and the culture condition is dark culture, 25±2℃;

[0019] (4) After the petiole callus was subcultured in the original medium for 30 days, it was transferred to the callus differentiation medium. The callus differentiation medium u...

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Abstract

The invention discloses a tissue culture method of spathiphyllum kochii petioles. The method comprises the steps as follows: spathiphyllum kochii is obtained with petioles of the spathiphyllum kochiias explants through disinfection and cleaning, callus culture, callus differentiation culture and rooting culture. The spathiphyllum kochii with good growth condition and low production cost can be obtained, the rooting induction rate is 100% in a sugar-free culture medium for rooting culture, and technical support is provided for cost saving of industrial production.

Description

technical field [0001] The invention relates to a method for culturing petiole tissue of white crane lily, belonging to the technical field of petiole tissue culture of white crane lily. Background technique [0002] Spathiphyllum kochii (scientific name: Spathiphyllum kochii) is a plant of the genus Spathiphyllum kochii in the Araceae family. Its aliases are: white palm, smooth sailing and bract leaf taro; its dark green leaves and white spathe are particularly fresh and elegant, and it is a good view of leaves. Flower plant; it can also absorb toxic gases such as formaldehyde, ammonia and stupid in the air, and has the effect of purifying the air. It is a flower suitable for indoor cultivation. However, the existing ramet propagation is a conventional propagation method, which hinders the promotion of good varieties and the development of new varieties. Existing technology also has the research that mainly carries out the multiplication technology around the terminal bud ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴高殷王晓邹薇薇魏春芳
Owner GUIZHOU UNIV
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