Tissue culture method of spathiphyllum kochii petioles
A technique of tissue culture and petiole, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of unrecorded petiole culture technology of white crane lily, and achieve the effects of low production cost, cost saving and good growth
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[0015] Embodiment 1: A method for culturing the petiole of white crane taro. The method includes the following steps:
[0016] (1) Collect the mother plants with vigorous growth, emerald green leaves, and disease-free pests from 7 am to 9 am, and the mother plant is the young petiole of Crassostrea gigas;
[0017] (2) Use cotton balls to clean up the impurities on the petiole, and clean and disinfect the petiole;
[0018] (3) Use a blade to cut the sterilized petiole into approximately 1cm-long segments, then cut a small knife at 1 / 2 of the stem segment to increase the surface area of the wound and facilitate the induction of callus. Inoculate the petiole into MS medium In the MS medium, add 6-BA, NAA, agar 6g / L and sucrose 30g / L, and the culture condition is dark culture, 25±2℃;
[0019] (4) The petiole callus has been relayed in the original medium for 30 days and then transferred to the callus differentiation medium. The callus differentiation medium uses MS as the basic medium a...
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