Tick-derived gene engineering vaccine

A tick and immunogen technology, applied in the field of vaccines, can solve problems such as drug residues, human health hazards, and environmental pollution

Inactive Publication Date: 2019-04-05
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But still not getting good results
Therefore, the current prevention and control of ticks is still dominated by chemical drugs. Trad

Method used

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  • Tick-derived gene engineering vaccine
  • Tick-derived gene engineering vaccine
  • Tick-derived gene engineering vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Screening target gene fragments based on the blood tick cDNA library

[0028] Firstly, the partial sequence of the BUD gene was screened using the sequence sequence of the blood tick cDNA library preserved by the applicant, and the 5' end and 3' end RACE primers were designed. The full-length gene sequence of tick-derived BUD was amplified by PCR. In order to obtain the best candidate gene, the present invention further amplified the BUD gene of Qinghai blood tick and leather tick, and analyzed the gene sequence by using bioinformatics methods. It is determined that the BUD gene is a kind of conserved gene in ticks and plays a key role in body protection. Then select BUD gene as the candidate gene of genetic engineering vaccine.

[0029] 2. Synthetic primers

[0030] According to the partial sequence of BUD in the blood tick cDNA library, the 5' and 3' RACE primer sequences were designed to annotate 5'RACE and 3'RACE, respectively. The primer sequences for the ful...

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Abstract

The invention provides an immunogen. The immunogen comprises an antigen BUD peptide connected with a carrier, wherein an amino acid sequence of the antigen BUD peptide is SEQ ID No. 2 in a sequence table. The invention further provides corresponding immunogenic composition and pharmaceutical composition, and application of the immunogenic composition and the pharmaceutical composition to the preparation of a medicine for controlling tick breeding. After recombinant protein pGEX-4T-1+BUD is immunized, a comprehensive evaluation result shows that gene engineering vaccine can effectively influence the development of ticks and the quantity of the ticks is extremely reduced.

Description

technical field [0001] The invention belongs to the technical field of vaccines, in particular to a tick-derived genetic engineering vaccine. Background technique [0002] As wild arthropods, ticks belong to the class Arachnida and the order Acarina. Adults have a scutellum with strong chitin on the back of the body, which is commonly called hard ticks and belongs to the family Ixodidae; those without scutum are commonly called soft ticks. Is a soft tick family. More than 800 species have been found in the world, including about 700 species of Ixodidae, about 150 species of Soft Ticks, and 1 species of Narthidae (only in Europe). There are about 100 species of Ixodidae and 10 species of Soft Ticks recorded in China. Ticks are transient parasites on the surface of many vertebrates, and they are the vectors and storage hosts of some zoonotic diseases. Ticks can not only cause mechanical damage to animals through biting, but also transmit diseases such as forest encephalitis...

Claims

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Application Information

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IPC IPC(8): C12N9/02C12N15/53C12N15/70C12N1/21A61K39/00A61P33/14
CPCA61K39/0003A61P33/14C12N9/0069C12N15/70C12Y113/12019
Inventor 罗金刘光远沈辉任巧云陈泽罗建勋殷宏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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