Umbilical cord mesenchymal stem cell factor freeze-dried powder and preparation method thereof

A technology of mesenchymal stem cells and freeze-dried powder, which is applied in the field of umbilical cord mesenchymal stem cell factor freeze-dried powder and its preparation, can solve the problems of complex protein components, microbial infection, and many invalid proteins, so as to achieve faster proliferation and improve secretion capacity , the effect of low immune rejection
CN109593124AActive Publication Date: 2019-04-09GUANGZHOU RAINHOME PHARM&TECH CO LTD

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU RAINHOME PHARM&TECH CO LTD
Publication Date
2019-04-09

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Abstract

The invention provides an umbilical cord mesenchymal stem cell factor freeze-dried powder and a preparation method thereof. Umbilical cord mesenchymal stem cells are cultured by mechanical stimulationand hypoxia stimulation to promote secretion of stem cell factors, and reasonable combination of components such as skimmed milk powder, trehalose, carboxymethyl cellulose, hydroxyethyl starch, glutamine and poloxamer is used as a freeze-dried powder protective agent. The prepared expected mesenchymal stem cell factor freeze-dried powder has good stability, stem cell factor content and activity.
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Description

technical field

[0001] The invention belongs to the field of biological technology, in particular to a freeze-dried powder of umbilical cord mesenchymal stem cell factor and a preparation method thereof. Background technique

[0002] During the stable growth process of stem cells, many kinds of cytokines are secreted into the medium supernatant in the form of protein peptides. By collecting and treating the supernatant after culturing stem cells, a large number of cytokines can be obtained through a series of treatments. These people Derived factors include EGF (epidermal growth factor), VEGF (vascular endothelial growth factor), HGF (liver growth factor), PDGF (platelet-derived factor), bFGF (fibroblast growth factor) and the like. Most of these factors have biological activities related to skin growth, blood vessel growth, and subcutaneous tissue regeneration. They can effectively regulate body cell signal transduction, activate human cells, and then physiologically repair...

Claims

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