A tumor model of Drosophila melanogaster and its application

A technology for fruit flies and tumors, applied in animal husbandry, etc., can solve the problems of not considering the influence of generations and gender, not being able to indicate the actual health risks of pollutants, and not being able to highlight pollutants that induce cancer, etc., to achieve operability and convenience Sexuality, widening the test organisms, and achieving the effect of coverage

Active Publication Date: 2020-07-14
TONGJI UNIV
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Problems solved by technology

Although the above tumor migration model helps to explain the mechanism of tumor migration and emphasizes theoretical research, it cannot highlight the pollution-induced cancer and cannot indicate the actual health risks of pollutant exposure; moreover, the above-mentioned tumor migration model does not consider the influence of generation and gender

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  • A tumor model of Drosophila melanogaster and its application
  • A tumor model of Drosophila melanogaster and its application
  • A tumor model of Drosophila melanogaster and its application

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Embodiment Construction

[0033] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0034] 1. Single-generation carcinogenic toxicity effects of pollutants applied to the Drosophila melanogaster tumor model strain: firstly, the gradient concentration of pollutants and the blank control were prepared. Gradient concentrations of 0.1, 10, and 1000 μg / L lindane and a blank control group were prepared, which contained 0.1% volume of dimethyl sulfoxide in water.

[0035] The pollution-free medium contains brown sugar, corn flour, yeast, agar and propionic acid. For every 1L of medium prepared, 135g of brown sugar and 7g of agar powder should be taken, dissolved in water, and the mixture is poured into a...

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Abstract

The invention provides application of a drosophila melanogaster tumor model in studying single-generation and multi-generation carcinogenic toxicity of environmental pollutants, and a method for detecting carcinogenic effect variation of the pollutants upon living organisms through single-generation and multi-generation exposure of the drosophila melanogaster tumor model in cancerogen or environmental pollution substances with potential carcinogenic effects. The invention confirms feasibility that environmental pollutants are applied to a drosophila melanogaster model to evaluate carcinogeniceffects, an exposure dosage is approximate to an actual environment concentration, in addition, inter-generation carcinogenic effect differences of single-generation exposure and multi-generation exposure are compared, and the effects of intensive and weak toxicity conduction of the pollutants upon drosophila melanogaster carcinogenic toxicities can be achieved. By adopting the method, unicity andethics limits of a conventional carcinogenic toxicity evaluation method are overcome, indexes of conventional toxicity evaluation on pollutants are expanded, the model has feasibility, and evaluationon toxicities of the pollutants of low concentrations and long-term living organism exposure is created and complemented.

Description

technical field [0001] The invention relates to the technical fields of ecotoxicology, environmental protection, ecological risk assessment, etc., in particular to a single-generation and multi-generation carcinogenic risk assessment method for pollutants using a Drosophila melanogaster tumor model. Background technique [0002] Drosophila melanogaster (Drosophila melanogaster) is an ideal model organism. It has the advantages of small size, strong fecundity, short growth cycle, easy feeding and manipulation, etc. It is widely used in genetic research and is also an advantageous organism for multi-generational research. . However, in the field of traditional toxicology, Drosophila melanogaster is not commonly used in the evaluation of toxic effects. Nevertheless, Drosophila melanogaster and mammals are highly conserved in tumor signaling pathways, and about 75% of human disease genes can find homologous genes in Drosophila. Therefore, Drosophila melanogaster is considered ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 于振洋沈佳颖尹大强史嘉琳胡子欣邱彬洋
Owner TONGJI UNIV
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