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Extraction method of insect bioactive protein

A bioactive protein and extraction method technology, applied in the field of extraction of natural active ingredients, can solve the problems of increased post-processing production costs, high requirements for reaction equipment, and reduced separation effects, so as to maintain activity without deterioration, ensure activity without deterioration, and ensure non-disruptive effect

Inactive Publication Date: 2019-04-12
中商国能孵化器集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, although the alkaline extraction has a high extraction rate, simple extraction, and low production cost, under the condition of high alkali, lysine-alanine will be generated in the protein extraction solution, which is not only poisonous, but also Causes the loss of nutrients; and under high alkali conditions, it will also cause protein hydrolysis, and accelerate the Maillard reaction, resulting in dark brown substances, which will cause the color of the extract to deepen, the non-protein content in the extract will increase, and the separation effect will decrease.
In addition, the existing alkaline and salt extraction methods have high requirements for reaction equipment, and will cause serious environmental pollution and increase the production cost of post-processing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 centipede protein extracts

[0024] The extraction of centipede protein described in this example is carried out based on the enzymatic hydrolysis method commonly used in the prior art, such as the extraction scheme of centipede protein described in Chinese patent CN107669844A, which specifically includes the following steps:

[0025] (1) In a fully enclosed state, under the condition of ensuring the isolation of the air and the isolation of the staff, the ambient temperature is controlled at 20-26 ° C, and the workshop for the extraction operation is sterilized;

[0026] (2) Then keep the entire extraction workshop in a closed space, and control the indoor temperature of the extraction workshop to be 30-38°C for the extraction of insect protein; the specific extraction steps include:

[0027] Fresh and live centipede adults were frozen with liquid nitrogen for 30 minutes, taken out and ground into powder, and the powder was poured into a centrifuge tube, a...

Embodiment 2

[0037] The extraction of the scorpion protein described in this embodiment is carried out based on the water extraction method commonly used in the prior art,

[0038] The extraction scheme of the scorpion protein as recorded in the Chinese patent CN103396472A specifically includes the following steps:

[0039] (1) In a fully enclosed state, under the condition of ensuring the isolation of the air and the isolation of the staff, the ambient temperature is controlled at 20-26 ° C, and the workshop for the extraction operation is sterilized;

[0040] (2) Then keep the entire extraction workshop in a closed space, and control the indoor temperature of the extraction workshop to be 30-38°C for the extraction of insect protein; the specific extraction steps include:

[0041] Take some medicinal materials of scorpion, soak in water for 30 minutes, extract by wet ultrafine grinding for 10 minutes, centrifuge the extract at 8000r / min for 10 minutes, take the supernatant, pass it throu...

Embodiment 3

[0049] The extraction of bufon protein described in this embodiment is carried out based on the alcohol extraction method commonly used in the prior art,

[0050] For example, the extraction scheme of venom protein described in Chinese patent CN102210711A specifically includes the following steps:

[0051] (1) In a fully enclosed state, under the condition of ensuring the isolation of the air and the isolation of the staff, the ambient temperature is controlled at 20-26 ° C, and the workshop for the extraction operation is sterilized;

[0052] (2) Then keep the entire extraction workshop in a closed space, and control the indoor temperature of the extraction workshop to be 30-38°C for the extraction of insect protein; the specific extraction steps include:

[0053] Take toad venom and wash it, add 10 times the amount of 95% (W / W) ethanol, reflux extraction twice, each time for 1.5 hours, filter and combine the filtrate, recover ethanol, concentrate to obtain the protein liquid...

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Abstract

The invention belongs to the field of extraction of natural active components, and particularly relates to an extraction method of insect bioactive protein. The extraction method of insect bioactive protein lies in that based on a conventional insect protein extraction method, isolated sterilization treatment is performed on an extraction workshop, so that the condition that extraction environmentis free from interference is effectively guaranteed; besides, the temperature control in an extracting process is controlled, so that the properties of extraction temperature are effectively guaranteed; and effective preservation is performed on extracted protein liquid in a gradient frozen manner or a freeze-dried manner, so that the activity of extracted protein can be effectively improved, andthe condition that activity of extracted protein can be maintained undeteriorated is facilitated.

Description

technical field [0001] The invention belongs to the field of extraction of natural active ingredients, and in particular relates to an extraction method of insect bioactive protein. Background technique [0002] According to statistics, there are more than 1 million species of insects in the world, of which more than 3,650 species are known to be edible. Insects have the characteristics of many types, large numbers, wide distribution, and rapid reproduction. Insects contain high protein, low fat, and low cholesterol, and have the advantages of reasonable nutritional structure and less meat fiber. And attracted the attention of countries all over the world. Experts predict that by the 21st century, insects will become the third largest source of protein after microorganisms and cell organisms. [0003] According to reports, the protein content of insects is higher than that of beef, pork, chicken, and fish. For example, dried wasp contains about 81% protein, bee 43%, cicad...

Claims

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Application Information

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IPC IPC(8): C07K1/14
CPCC07K1/145Y02P20/54
Inventor 王海平
Owner 中商国能孵化器集团有限公司
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