Extraction method of insect bioactive protein
A bioactive protein and extraction method technology, applied in the field of extraction of natural active ingredients, can solve the problems of increased post-processing production costs, high requirements for reaction equipment, and reduced separation effects, so as to maintain activity without deterioration, ensure activity without deterioration, and ensure non-disruptive effect
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Embodiment 1
[0023] Embodiment 1 centipede protein extracts
[0024] The extraction of centipede protein described in this example is carried out based on the enzymatic hydrolysis method commonly used in the prior art, such as the extraction scheme of centipede protein described in Chinese patent CN107669844A, which specifically includes the following steps:
[0025] (1) In a fully enclosed state, under the condition of ensuring the isolation of the air and the isolation of the staff, the ambient temperature is controlled at 20-26 ° C, and the workshop for the extraction operation is sterilized;
[0026] (2) Then keep the entire extraction workshop in a closed space, and control the indoor temperature of the extraction workshop to be 30-38°C for the extraction of insect protein; the specific extraction steps include:
[0027] Fresh and live centipede adults were frozen with liquid nitrogen for 30 minutes, taken out and ground into powder, and the powder was poured into a centrifuge tube, a...
Embodiment 2
[0037] The extraction of the scorpion protein described in this embodiment is carried out based on the water extraction method commonly used in the prior art,
[0038] The extraction scheme of the scorpion protein as recorded in the Chinese patent CN103396472A specifically includes the following steps:
[0039] (1) In a fully enclosed state, under the condition of ensuring the isolation of the air and the isolation of the staff, the ambient temperature is controlled at 20-26 ° C, and the workshop for the extraction operation is sterilized;
[0040] (2) Then keep the entire extraction workshop in a closed space, and control the indoor temperature of the extraction workshop to be 30-38°C for the extraction of insect protein; the specific extraction steps include:
[0041] Take some medicinal materials of scorpion, soak in water for 30 minutes, extract by wet ultrafine grinding for 10 minutes, centrifuge the extract at 8000r / min for 10 minutes, take the supernatant, pass it throu...
Embodiment 3
[0049] The extraction of bufon protein described in this embodiment is carried out based on the alcohol extraction method commonly used in the prior art,
[0050] For example, the extraction scheme of venom protein described in Chinese patent CN102210711A specifically includes the following steps:
[0051] (1) In a fully enclosed state, under the condition of ensuring the isolation of the air and the isolation of the staff, the ambient temperature is controlled at 20-26 ° C, and the workshop for the extraction operation is sterilized;
[0052] (2) Then keep the entire extraction workshop in a closed space, and control the indoor temperature of the extraction workshop to be 30-38°C for the extraction of insect protein; the specific extraction steps include:
[0053] Take toad venom and wash it, add 10 times the amount of 95% (W / W) ethanol, reflux extraction twice, each time for 1.5 hours, filter and combine the filtrate, recover ethanol, concentrate to obtain the protein liquid...
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