A kind of biocontrol bacteria and its application in the control of downy mildew of crops
A bio-control bacteria and related technology, applied in the field of bio-control bacteria and in the field of crop downy mildew prevention and control, can solve the problems of food safety, environmental pollution, health threats, etc., to reduce powdery mildew, increase yield, improve crop planting micro The effect of ecological environment
Active Publication Date: 2021-12-31
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology
[0005] At present, the breeding work for downy mildew resistance has not made breakthrough progress. The main control measures for downy mildew in agricultural production are still spraying chemical agents, etc., and the main control measures for downy mildew are still spraying chemical agents. Pesticides include metalaxyl (Mefenoxam), methoxyacrylates (QoIs, such as azoxystrobin), etc., but the long-term high-frequency use of single-site fungicides has led to the emergence of a large number of drug-resistant physiological races of downy mildew At the same time, it brings serious food safety and environmental pollution problems, which seriously threatens our health
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[0020] The present invention will be made hereinafter by way of specific embodiments, but the present invention is not limited to these specific embodiments.
[0021] The materials, reagents, etc. used in the specific embodiments, can be obtained from the business pathway as not particularly illustrative. None of the specific techniques or conditions, according to the techniques or conditions described in the art (such as reference J. Sambruck waiting, Huang Peicang and other "molecular clones" third edition, scientific publishing house ) Or according to the product manual.
[0022] First, the following describes the present inventors isolated from the natural environment screened biocontrol AG11 present invention (Bacillus subtilis (Bacillus subtilis)) during operation of the CGMCC No.16732.
[0023] (1) isolating potential biocontrol
[0024] Biocontrol potentially present in the separation plant vitro:
[0025] Weigh cucumber (cucumber greenhouses collected from Huaiyin Distric...
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Abstract
The invention relates to a biocontrol bacterium and its application in the control of downy mildew of crops, and belongs to the technical field of crop biological control. The biocontrol fungus CGMCC No.16732 of the present invention can effectively prevent and treat downy mildew of crops, especially in the application of cucumber, soybean and grape planting, can effectively prevent and treat downy mildew of cucumber, soybean and grape; in addition, the inventor also found that The biocontrol bacterium CGMCC No.16732 of the present invention can also reduce the occurrence of crop powdery mildew, and has a growth-promoting effect on crops, and improves crop yield; moreover, the biocontrol bacterium of the present invention is compared with the chemical agents of the prior art, It is non-toxic to humans and animals, does not pollute the environment, and has no residue. In particular, it can improve the micro-ecological environment of crops and is conducive to the sustainable development of the ecological environment.
Description
Technical field [0001] The present invention relates to a method of biocontrol agents and their applications in crop downy mildew controlled, crops belonging to technical field of biological control. Background technique [0002] Downy mildew means that the plant diseases caused by fungi of downy mildew is widely distributed throughout the world, is also widely occur in our country, it is the major diseases of many crops, seriously affecting the quality and yield of crops, causing serious economic losses . [0003] Downy mildew are obligate parasites, there may cucumber downy mildew caused by Pseudoperonospora cubensis (Pseudoperonosporacubensis), causing soybean downy mildew downy mildew (Peronospora man-schurica) and downy mildew caused by grape vine downy mildew (Plasmopara viticola). [0004] Downy mildew of plants is generally lower leaves start onset and early stage of generating water-soaked light green dots, obvious lesion edge, irregular spots later development of a yell...
Claims
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IPC IPC(8): C12N1/20A01N63/22A01N47/24A01N37/50A01P3/00A01P21/00C12R1/125
CPCA01N37/50A01N47/24A01N63/00C12N1/20C12R2001/125C12N1/205
Inventor 郭坚华张丽娜蒋春号
Owner NANJING AGRICULTURAL UNIVERSITY
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