Method for establishing UPLC spectrum of radix achyranthis bidentatae and radix achyranthis bidentatae preparata with wine, and applications

A method for establishing a method, a technology of Achyranthes bidentata and wine Achyranthes bidentata, which is applied in the field of establishing UPLC spectra of Achyranthes bidentata and Achyranthes bidentata, which can solve problems such as cumbersome operation, long analysis time, and poor separation of chromatographic peaks, and achieve good stability and good repeatability Good performance and accuracy, UPLC detection separation

Active Publication Date: 2019-04-16
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of these prior art literature studies use HPLC analysis, which takes a long time to analyze. There are also literatures that perform UPLC-Q-TOF/MS analysis on chemica

Method used

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  • Method for establishing UPLC spectrum of radix achyranthis bidentatae and radix achyranthis bidentatae preparata with wine, and applications
  • Method for establishing UPLC spectrum of radix achyranthis bidentatae and radix achyranthis bidentatae preparata with wine, and applications
  • Method for establishing UPLC spectrum of radix achyranthis bidentatae and radix achyranthis bidentatae preparata with wine, and applications

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1 reagent material

[0033] Instrument: Waters ultra-high performance liquid chromatograph (Waters, Waters H-class), PDA detector (Waters); Empower workstation; 1 / 10,000 balance (Mettler-Toledo, ME204E) ; One millionth balance (Mettler-Toledo, XP26); Ultrapure water machine (Merck, Milli-Q Direct8 / 16system); Ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd., KQ5500DE); UPLC T3 (2.1×100mm, 1.6μm), electric ceramic furnace (Hangzhou Joyoung, H22-X3).

[0034] Reagents: ethanol (Tianjin Fuyu Fine Chemical Co., Ltd.), methanol (Tianjin Fuyu Fine Chemical Co., Ltd.), formic acid (Tianjin Kemiou Chemical Reagent Co., Ltd.), acetonitrile (Merck Co., Ltd.), methanol ( Merck Co., Ltd.) was chromatographic grade; water was ultrapure water (Merck Co., Ltd., Milli-Q Direct), rice wine.

[0035] Test drug: β-ecdysterone (National Institute for Food and Drug Control, content: 98.40%, batch number: 111638-201706).

[0036] 2 Methods and results

[0037] 2.1 Achyranthes ...

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Abstract

The invention provides a method for establishing a UPLC spectrum of radix achyranthis bidentatae and radix achyranthis bidentatae preparata with wine. The method for establishing a UPLC spectrum of radix achyranthis bidentatae and radix achyranthis bidentatae preparata with wine comprises the following steps: preparation of a test solution: taking radix achyranthis bidentatae or radix achyranthisbidentatae preparata with wine test sample powder, adding 10% methanol, carrying out ultrasonic treatment, filtering, and taking filtrate to obtain the test solution; and detecting by adopting a highperformance liquid chromatograph, namely detecting the test solution by adopting a Waters high performance liquid chromatograph, and establishing a UPLC spectrum. Methods for identifying comprise thesteps of detecting whether 5-hydroxymethylfurfural exists in a test solution or not; and if 5-hydroxymethylfurfural is detected, identifying the sample as radix achyranthis bidentatae preparata with wine; alternatively, analyzing whether characteristic peaks appear within the relative retention time ranges of 0.27+/-10%, 0.37+/-10% and 0.41+/-10% or not by taking beta-ecdysterone as a reference peak in the UPLC spectrum, and if one or more characteristic peaks appear, identifying the sample as the radix achyranthis bidentatae preparata with wine.

Description

technical field [0001] The invention relates to a method for establishing UPLC spectra of Achyranthes bidentata and wine achyranthes bidentata and an application method for distinguishing achyranthes bidentata and wine achyranthes bidentata. Background technique [0002] Achyranthes is the dry root of Achyranthes bidentata Bl. Its nature is flat, bitter and sour, and it returns to the liver and kidney meridians. It has the effects of promoting blood circulation, tonifying liver and kidney, strengthening muscles and bones, diuresis and treating stranguria, and drawing blood (fire) downward. It was first recorded in "Shen Nong's Materia Medica", listed as top grade, also known as Baibei, and "Guang Ya" called it Niu stem. Achyranthes bidentata has a long history of medicinal use, and the "Compendium of Materia Medica" records that "the seedlings' side stems have violent joints...the spikes and seeds...all stick to the stems and grow backwards". Tao Hongjing interpreted the n...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 黎晓丽杨晓东梁慧罗文汇罗宇琴杨文惠马瑞瑞陈向东
Owner GUANGDONG YIFANG PHARMA
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