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Antibody diluent for enhancing use effect of PD-L1 monoclonal antibody and use method of antibody diluent

A PD-L1, antibody diluent technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of software system exclusivity, high price of automatic immunohistochemical staining instrument, etc., to enhance the role of molecular dynamics , Enhance the intensity of immunohistochemical staining, reduce the effect of harmful waste liquid

Active Publication Date: 2019-04-23
AMOY DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high price of the fully automatic immunohistochemical staining instrument and the exclusivity of its software system, the popularity and consistency of PD-L1 immunohistochemical detection have caused huge problems

Method used

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  • Antibody diluent for enhancing use effect of PD-L1 monoclonal antibody and use method of antibody diluent
  • Antibody diluent for enhancing use effect of PD-L1 monoclonal antibody and use method of antibody diluent
  • Antibody diluent for enhancing use effect of PD-L1 monoclonal antibody and use method of antibody diluent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Antibody diluent configuration, which includes: PEG20000 (1g / L), casein sodium salt (5mg / L), acetaminophen (5mg / L), bovine serum albumin (100mg / L), sodium chloride (5mM) , 0.1% TritonX, 0.1% Proclin300.

[0027] After each component is weighed, it is dissolved in water respectively, mixed to the above concentration, and then the pH of the solution is adjusted to 8.

Embodiment 2

[0029] Antibody diluent configuration, which includes: PEG20000 (0.5g), casein sodium salt (50mg), acetaminophen (50mg), bovine serum albumin (1000mg), sodium chloride (100mM), 1% TritonX, 1% Proclin300.

[0030] After each component is weighed, it is dissolved in water and mixed to the above concentration, and then the pH of the solution is adjusted to 8.1. .

Embodiment 3

[0032] Antibody diluent configuration, which includes: PEG20000 (0.5g), casein sodium salt (100mg), acetaminophen (500mg), bovine serum albumin (500mg), sodium chloride (300mM), 5% TritonX, 3% Proclin300. After each component is weighed, it is dissolved in water and mixed to the above concentration, and then the pH of the solution is adjusted to 7.8.

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Abstract

The invention discloses an antibody diluent for enhancing the use effect of a PD-L1 monoclonal antibody and a use method of the antibody diluent. The antibody diluent is prepared from the components in percentage by mass: 0.1-100 g / L of PEG 20000, 1-1000 mg / L of casein sodium salt, 1-1000 mg / L of acetaminophen, 10-10000 mg / L of bovine serum albumin, 1-1000 mM of sodium chloride, 0.01-10% of TritonX, 0.01-10% of Proclin300, and a water solvent. The antibody diluent can enhance the use effect of the PD-L1 monoclonal antibody.

Description

technical field [0001] The present invention relates to an antibody diluent for enhancing the effect of PD-L1 monoclonal antibody and its use method. Background technique [0002] Immunohistochemistry is the use of the principle of specific binding of antigens and antibodies to determine the antigens (polypeptides and proteins) in tissue cells through chemical reactions to develop the color of the chromogenic agent of the labeled antibodies, and to conduct localization, qualitative and quantitative research on them, referred to as For immunohistochemistry. At present, immunohistochemical staining methods are mainly divided into: manual staining completed by the experimenter and instrumental staining completed by the automatic immunohistochemical staining machine. [0003] PD-L1 is the most important target in tumor immunotherapy, and the expression of PD-L1 in tumors is closely related to the therapeutic effect of PD-1 / L1 antibody drugs. The clinical detection method of PD...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/536
CPCG01N33/536G01N33/577
Inventor 朱俊轩周文刚陈宁郑立谋
Owner AMOY DIAGNOSTICS CO LTD
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