A kind of preparation method of Sutong potato culture medium conducive to the growth of BCG floating film

A technology of potato culture medium and Sutong medium, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of inoculation difficulty, subculture difficulty, easy paste soup, etc., and achieve convenient cleaning, drying, Effects of increasing bacterial cell yield and reducing starch content

Active Publication Date: 2022-07-12
成都可恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Sutong potato culture medium prepared according to this method causes potatoes to be easily paste after autoclaving, and the potatoes are soft and rotten, which makes it difficult to inoculate. The vertical distance increases, making it difficult for BCG to grow to the liquid level of the Sutong medium, resulting in difficulties in subculture

Method used

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  • A kind of preparation method of Sutong potato culture medium conducive to the growth of BCG floating film
  • A kind of preparation method of Sutong potato culture medium conducive to the growth of BCG floating film
  • A kind of preparation method of Sutong potato culture medium conducive to the growth of BCG floating film

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Take a potato with a starch content of ≤13%, which is not easy to paste soup, and cut it into a semi-cylinder with a diameter of 2.0 cm and a length of 3.0 cm with a mold. The dividing point is at 1 / 2 of the length of the semi-cylindrical body, which is cut into double inclined planes. Soak the cut potatoes in purified water for 10 minutes, then replace the purified water and take a boiling water bath for 15 minutes. Put the potato pieces into the necked test tube (2.5cm×20cm), add 10ml of Sutong medium to the necked test tube, plug the gas-permeable rubber stopper, and lean against the culture plate, so that the upper slope of the potato pieces is horizontal. The medium is connected to the lower slope of the potato piece, and the vertical distance below the upper slope of the potato piece is 0.1-0.3 cm, and the following results are obtained: figure 1 The shown Sutong potato medium includes a necked test tube 1, a Sutong medium 2, a new type of potato block (double sl...

Embodiment 2

[0029] Take a potato with a starch content of ≤13%, which is not easy to paste soup, and cut it into a semi-cylinder with a diameter of 1.7cm and a length of 2.5cm with a mold. The dividing point is 2 / 5 of the length of the semi-cylindrical body, which is cut into double inclined planes. Soak the cut potatoes in purified water for 8 minutes, then replace the purified water and take a boiling water bath for 10 minutes. Put the potato pieces into the necked test tube (2.5cm×20cm), add 8ml of Sutong medium to the necked test tube, plug the gas-permeable rubber stopper, and lean against the culture plate, so that the upper slope of the potato pieces is horizontal, and the The medium is connected to the lower slope of the potato piece, and the vertical distance below the upper slope of the potato piece is 0.1-0.3 cm, and the following results are obtained: figure 1 The shown Sutong potato medium includes a necked test tube 1, a Sutong medium 2, a new type of potato block (double s...

Embodiment 3

[0031] Take a potato with a starch content of ≤13%, which is not easy to paste soup, and cut it into a semi-cylinder with a diameter of 2.3cm and a length of 3.5cm with a mold. The dividing point is 3 / 5 of the length of the semi-cylindrical body, which is cut into double inclined planes. Soak the cut potatoes in purified water for 15 minutes, then replace the purified water and take a boiling water bath for 12 minutes. Put the potato pieces into the necked test tube (2.5cm×20cm), add 12ml of Sutong medium to the necked test tube, plug the gas-permeable rubber stopper, and lean against the culture plate, so that the upper slope of the potato pieces is horizontal. The medium is connected to the lower slope of the potato piece, and the vertical distance below the upper slope of the potato piece is 0.1-0.3 cm, and the following results are obtained: figure 1 The shown Sutong potato medium includes a necked test tube 1, a Sutong medium 2, a new type of potato block (double slope) ...

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Abstract

The invention relates to a preparation method of Sutong potato culture medium which is beneficial to the growth of BCG slick. The invention provides a new preparation process of Sutong potato culture medium which must be used in the subculture of BCG cm half cylinder, and then cut into double bevels at 2 / 5~3 / 5 of the length of the half cylinder as the dividing point. Soak in purified water for 8 to 15 minutes, and then replace the purified water to wash away starch and prevent enzymatic browning of potatoes. Then boil the cut potatoes for 10-15min to further prevent enzymatic browning of the potatoes. Put the potato pieces into the necked test tube (2.5cm×20cm), add 8~12ml of Sutong medium to the necked test tube, plug the gas-permeable rubber stopper, put it at 116℃, and autoclave for 30min. The BCG cultured in the Sutong potato medium prepared by the method is beneficial to the growth of the floating film to the liquid level of the Sutong medium, thereby increasing the yield, and at the same time, the preparation is simple, and the inoculation and use are convenient.

Description

technical field [0001] The invention belongs to the technical field of biological product production, and in particular relates to a method for preparing a Sutong potato culture medium which is beneficial to the growth of BCG smears. Background technique [0002] BCG for therapeutic use BCG working seeds batches are cultured in Roche egg medium, Sutong potato medium, Sutong medium, and Sutong medium, and then collect the bacteria, add stabilizers and distribute them into vials. Freeze-dried immunotherapeutics. Studies have shown that therapeutic BCG can enhance the activity of macrophages, enhance the ability of macrophages to kill tumor cells, activate T lymphocytes, and enhance the body's cellular immunity. It can be used for ① adjuvant therapy of tumors; ② prevention of tuberculosis; ③ treatment of asthmatic bronchitis and prevention of colds in children. Also used for the treatment of carcinoma in situ of the bladder and the prevention of recurrence, for the prevention...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12M1/24C12M1/00C12R1/32
Inventor 严秋萍任昭源马昭明陈山岭张雪莹吴盈盈李泳宏陈楠
Owner 成都可恩生物科技有限公司
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