A kind of preparation method of Sutong potato culture medium conducive to the growth of BCG floating film
A technology of potato culture medium and Sutong medium, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of inoculation difficulty, subculture difficulty, easy paste soup, etc., and achieve convenient cleaning, drying, Effects of increasing bacterial cell yield and reducing starch content
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Embodiment 1
[0027] Take a potato with a starch content of ≤13%, which is not easy to paste soup, and cut it into a semi-cylinder with a diameter of 2.0 cm and a length of 3.0 cm with a mold. The dividing point is at 1 / 2 of the length of the semi-cylindrical body, which is cut into double inclined planes. Soak the cut potatoes in purified water for 10 minutes, then replace the purified water and take a boiling water bath for 15 minutes. Put the potato pieces into the necked test tube (2.5cm×20cm), add 10ml of Sutong medium to the necked test tube, plug the gas-permeable rubber stopper, and lean against the culture plate, so that the upper slope of the potato pieces is horizontal. The medium is connected to the lower slope of the potato piece, and the vertical distance below the upper slope of the potato piece is 0.1-0.3 cm, and the following results are obtained: figure 1 The shown Sutong potato medium includes a necked test tube 1, a Sutong medium 2, a new type of potato block (double sl...
Embodiment 2
[0029] Take a potato with a starch content of ≤13%, which is not easy to paste soup, and cut it into a semi-cylinder with a diameter of 1.7cm and a length of 2.5cm with a mold. The dividing point is 2 / 5 of the length of the semi-cylindrical body, which is cut into double inclined planes. Soak the cut potatoes in purified water for 8 minutes, then replace the purified water and take a boiling water bath for 10 minutes. Put the potato pieces into the necked test tube (2.5cm×20cm), add 8ml of Sutong medium to the necked test tube, plug the gas-permeable rubber stopper, and lean against the culture plate, so that the upper slope of the potato pieces is horizontal, and the The medium is connected to the lower slope of the potato piece, and the vertical distance below the upper slope of the potato piece is 0.1-0.3 cm, and the following results are obtained: figure 1 The shown Sutong potato medium includes a necked test tube 1, a Sutong medium 2, a new type of potato block (double s...
Embodiment 3
[0031] Take a potato with a starch content of ≤13%, which is not easy to paste soup, and cut it into a semi-cylinder with a diameter of 2.3cm and a length of 3.5cm with a mold. The dividing point is 3 / 5 of the length of the semi-cylindrical body, which is cut into double inclined planes. Soak the cut potatoes in purified water for 15 minutes, then replace the purified water and take a boiling water bath for 12 minutes. Put the potato pieces into the necked test tube (2.5cm×20cm), add 12ml of Sutong medium to the necked test tube, plug the gas-permeable rubber stopper, and lean against the culture plate, so that the upper slope of the potato pieces is horizontal. The medium is connected to the lower slope of the potato piece, and the vertical distance below the upper slope of the potato piece is 0.1-0.3 cm, and the following results are obtained: figure 1 The shown Sutong potato medium includes a necked test tube 1, a Sutong medium 2, a new type of potato block (double slope) ...
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