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A detection method of traditional Chinese medicine fingerprints of compound Togucao preparation

A technology of fingerprint spectrum and detection method, applied in the field of quality detection of traditional Chinese medicine preparations, can solve problems such as no relevant reports, and achieve the effects of simple operation, high degree of separation, and objective conclusion

Active Publication Date: 2021-01-12
YUEYANG INTEGRATED TRADITIONAL CHINESE & WESTERN MEDICINE HOSPITAL SHANGHAI UNIV OF CHINESE TRADITIONAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It is very necessary to establish the traditional Chinese medicine fingerprint and its detection method of the compound Togucao preparation, but there is no relevant report so far

Method used

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  • A detection method of traditional Chinese medicine fingerprints of compound Togucao preparation
  • A detection method of traditional Chinese medicine fingerprints of compound Togucao preparation
  • A detection method of traditional Chinese medicine fingerprints of compound Togucao preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] The establishment of the Chinese medicine standard fingerprint of the compound Togucao preparation of embodiment 1

[0078] 1. Name

[0079] Compound Speranto solution.

[0080] 2. The source of the test product

[0081] Shanghai University of Traditional Chinese Medicine, batch number: 1703021, 1703022, 1703023, 1703061, 1703062, 1707071, 1707121, 1707122, 1707123, 1707171.

[0082] 3. Preparation of the test solution

[0083] Measure 1.5ml of 10 batches of the compound Speranthema solution, add 3ml of methanol, shake well, pass through a 0.22μm organic microporous membrane after standing, and take the subsequent filtrate to obtain the test solution.

[0084] 4. Preparation of reference solution

[0085] The main active ingredients of the compound Speranthes solution are rutin, quercetin and hyperin. After experimental research, sodium benzoate with stable peak time and moderate peak area was selected as a reference. Reference substance source: hyperin (111521-20...

Embodiment 2

[0151]This example is a method for detecting the fingerprint of traditional Chinese medicines for the compound Togucao preparation. The compound Speranthema preparation is a solution. Specifically, after mixing 55g of alum, 60g of Zanthoxylum bungeanum, 5g of coriander seed, 25g of saponins, and 25g of Scutellaria osmanthus, soak with an appropriate amount of rice vinegar for 6 days, stir in due course, filter, and the filtrate Add rice vinegar to adjust the total amount to 1000ml. The Chinese medicine fingerprint detection method comprises the following steps:

[0152] (1) Preparation of reference substance solution

[0153] Weigh rutin, quercetin, hyperin and sodium benzoate reference substances, add methanol to dissolve, and make a 1 μg / ml reference substance solution;

[0154] (2) preparation of need testing solution

[0155] Measure 1ml of the compound Speranthema preparation, add 6ml of methanol, mix well, pass through a 0.22 μm organic microporous filter membrane aft...

Embodiment 3

[0166] This example is a method for detecting the fingerprint of traditional Chinese medicines for the compound Togucao preparation. The compound Speranthema preparation is a solution. Specifically, after mixing 65g of alum, 58g of Zanthoxylum bungeanum, 7g of coriander seed, 35g of saponins, and 35g of Speranthema, soak with appropriate amount of rice vinegar for 8 days, stir in due course, filter, and the filtrate Add rice vinegar to adjust the total amount to 1000ml. The Chinese medicine fingerprint detection method comprises the following steps:

[0167] (1) Preparation of reference substance solution

[0168] Weigh rutin, quercetin, hyperin and sodium benzoate reference substances, add methanol to dissolve, and make a 300 μg / ml reference substance solution;

[0169] (2) preparation of need testing solution

[0170] Measure 2ml of the compound Speranthema preparation, add 3ml of methanol, mix evenly, pass through a 0.22 μm organic microporous filter membrane after stand...

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Abstract

The invention relates to a traditional Chinese medicine fingerprint spectrum inspection method of compound garden balsam stem preparations. The traditional Chinese medicine fingerprint spectrum inspection method of the compound garden balsam stem preparations comprises the steps that (1) a reference substance solution is prepared; (2) a to-be-tested substance solution is prepared; and (3) a high performance liquid chromatography is adopted for measurement, specifically, chromatographic conditions are that a chromatographic column is a C18 column, column temperature is 20-40 DEG C, a mobile phase is a 0.05-0.15 vol% phosphoric acid water solution and acetonitrile for gradient elution, and the measuring wavelength is 210-240 nm. A standardized fingerprint spectrum is established based on multiple batches of the compound garden balsam stem preparations, and a quality inspecting method of the compound garden balsam stem preparations is provided. The traditional Chinese medicine fingerprintspectrum inspection method can be used to instruct the operation of batch charging in the production and preparation process and standardize production operation, the integrity of the quality controlin the whole process can be ensured all the time, and safety, efficiency and stability of clinical medication are ensured.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicine preparations, in particular to a method for detecting the fingerprints of traditional Chinese medicines for compound Togucao preparations. Background technique [0002] Compound Speranthema solution is a hospital preparation registered in Shanghai Food and Drug Administration by Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine. The drug standard number is: SYZ-ZF-271-2005. This hospital also applied for a Chinese invention patent for the drug composition, the publication number is CN104000919A, and the publication date is 2014.08.27. The composition of the compound Speranthum preparation is: 20-40 parts of Speranthum, 50-70 parts of Zanthoxylum bungeanum, 20-40 parts of saponins, 4-8 parts of wood turtle, and alum. The weight of the above medicinal ingredients and alum The rati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/86G01N30/74G01N30/06
Inventor 范斌张宁张洁玉钟丛丛
Owner YUEYANG INTEGRATED TRADITIONAL CHINESE & WESTERN MEDICINE HOSPITAL SHANGHAI UNIV OF CHINESE TRADITIONAL MEDICINE
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