Method for constructing sugarcane brown rust resistance gene positioning genetic separation population

Abstract

The invention relates to a method for constructing a sugarcane brown rust resistance gene positioning genetic separation population, which comprises the following steps: using sugarcane varieties withdifferent sources and high sensitivity to brown rust as female parents, using sugarcane varieties with high resistance to brown rust and no Bru1 gene as male parents, preparing, combining and hybridizing to obtain F1 generation seeds, sowing, screening a true hybrid F1 generation, planting a single plant of the true hybrid F1 generation, inoculating brown rust germs, investigating the disease incidence condition, evaluating the resistance according to a 9-grade standard, analyzing the disease resistance inheritance of a population of the true hybrid F1 generation, and selecting a true hybridF1 generation population of which the resistance-to-infection separation ratio of the hybrid offspring genetic separation population is approximately equal to 3:1 as the sugarcane brown rust resistance gene positioning genetic separation population. According to the method, a set of method for systematically constructing the sugarcane brown rust resistance gene positioning genetic separation population is established for the first time, the program is simplified, the operability is strong, the resistance-to-infection separation ratio of the constructed true hybrid F1 population is approximately equal to 3:1, the disease resistance gene of the constructed true hybrid F1 population is controlled by a dominant single gene, and the population can be used for gene positing of brown rust resistance.

Description

technical field [0001] The invention belongs to the technical field of crop disease-resistant gene positioning, and in particular relates to a method for constructing a genetic isolation population of sugarcane brown rust resistance gene positioning. Background technique [0002] Sugarcane brown rust is an important disease caused by Puccina.melanocephala H.Sydow&P.Sydow. It often occurs in the main sugarcane producing areas of my country, causing huge economic losses and seriously affecting the reliability of the sugar industry. Continuous development. Sugarcane brown rust is mainly manifested as a large number of reddish-brown lesions on sugarcane leaves, and the lesions merge to form large amorphous necrotic areas. As a result, sugarcane leaves die before they are ripe, even young leaves. [0003] The prevalence of sugarcane brown rust is closely related to the disease resistance of varieties, and the large-scale planting of susceptible varieties is an important reason fo...

AI Technical Summary

Problems solved by technology

In terms of production, it is very difficult to control sugarcane brown rust with large-scale application of pesticides, and the results are minimal. Breeding and planting disease-resistant varieties is the most economical and effective measure to control sugarcane brown rust. Preliminary studies have found that some sugarcane varieties have high resistance to brown rust. , the discovered brown rust resistance gene Bru1 was not detected by molecular marker detection and analysis, indicating that these sugarcane varieties may contain new brown rust resistance genes, and the discovery and utilization of these disease-resistant germplasm resources is the basis for effective brown rust resistance breeding in sugarcane And the key is to use its germplasm resources with high resistance to brown rust to study and clarify the genetic rules of its resistance, and it is necessary to discover resistance gene resources