Application of fish oligopeptide in cosmetics
A fish oligopeptide and cosmetic technology, which is applied in the field of daily necessities to achieve the effects of good application prospects, easier absorption and skin damage repair
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[0069] Preparation Example Preparation of fish oligopeptide
[0070] Take 1000g of deep-sea fish skin (with fish scales), add 3000ml of pure water, and then heat the feed solution to 47°C; adjust the pH of the feed solution to 7.8 with a 10% NaOH solution by mass percent, and then add protease 3.0g (the protease is composed of AS1398 neutral protease, trypsin and bromelain in a weight ratio of 1:1:1), stirred for 5.5h. Afterwards, the temperature of the feed solution was raised to 65° C. and kept for 20 minutes to inactivate the enzyme, and then the feed solution was centrifuged at 4000 rpm for 10 minutes to retain the supernatant. Then add 3% (v / v) polyacrylamide flocculant solution with a mass percentage concentration of 1% to the clear liquid, and stir for 5 min, then centrifuge at 4000 rpm for 10 min, discard the precipitate, and keep the clear liquid. liquid. Then the clear liquid is subjected to electrodialysis, wherein the voltage is controlled at 65V, and the conduct...
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