PD1-knockout chimeric antigen receptor T cell targeting EGFR, preparation method and application thereof
A chimeric antigen receptor and targeting technology, applied in the field of medical biology
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0093] A method for preparing chimeric antigen receptor T cells targeting EGFR that knocks out PD1, comprising the following steps:
[0094] (1) Prepare the gene sequence of chimeric antigen receptor CAR-EGFR targeting EGFR
[0095] The genes encoding the signal peptide, the single-chain antibody targeting EGFR, the hinge region of CD8α, the transmembrane region of CD8, the signal region of 4-1BB and the signal region of CD3ζ were prepared respectively, and the genes encoding the signal peptide were shown in SEQ ID NO: 8 The coding gene of the single-chain antibody targeting EGFR is shown in SEQ ID NO: 5, the coding gene of the CD8 α hinge region is shown in SEQ ID NO: 10, and the coding gene of the CD8 transmembrane region As shown in SEQ ID NO: 12, the coding gene of the 4-1BB signal region is shown in SEQ ID NO: 14, and the coding gene of the CD3ζ signal region is shown in SEQ ID NO: 16, wherein the targeting The single-chain antibody to EGFR is a humanized single-chain an...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com