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Molecular markers tightly linked to broad-spectrum resistance to tomato scab and uses thereof

A tomato scab, tomato technology, applied in the field of molecular markers, can solve the problem of not being able to meet the needs of disease-resistant varieties

Active Publication Date: 2020-10-27
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these genes have been used in disease-resistant breeding, the bred varieties are only resistant to a single race, which cannot meet the needs of disease-resistant varieties with multiple races in tomato growing areas.

Method used

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  • Molecular markers tightly linked to broad-spectrum resistance to tomato scab and uses thereof
  • Molecular markers tightly linked to broad-spectrum resistance to tomato scab and uses thereof
  • Molecular markers tightly linked to broad-spectrum resistance to tomato scab and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1. Acquisition of Molecular Marker SlPub-Del Closely Linked to Tomato Broad-spectrum Scab Resistance Gene

[0055] The above spray inoculation method was used to evaluate the resistance of the resistant parent PI114490, the susceptible parent OH88119 and a self-backcross population (Scott et al.2003; Sun Huijun et al. 2011; Scott et al.2013) containing 166 lines. , according to the analysis results of significant differences in the disease level of each line, the anti-susceptibility response of each line was determined. The lines with a disease level significantly higher than PI114490 were susceptible, and the rest were resistant.

[0056] According to the existing results, there is a gene resistant to all species and races of scab in tomato material PI114490, which is located on chromosome 11 (Scott et al.2003; Sun Huijun et al. 2011; Scott et al.2013 ), using gene differential expression analysis techniques (cDNA-AFLP and RNA-seq), the gene SlPub24 was identif...

Embodiment 2

[0057] Example 2, the application of the molecular marker SlPub-Del closely linked with the tomato broad-spectrum anti-scab gene

[0058] In order to further verify the molecular marker SlPub-Del developed in Example 1 above, establish a marker-assisted selection system, and obtain materials resistant to tomato scab, this example uses the molecular marker SlPub-Del obtained in Example 1 above to pair 192 Marker analysis was performed on tomato materials (including PI114490) from different sources (Liu et al.2017, the public can obtain from China Agricultural University). The results showed that 16 materials (including PI114490) amplified to a band of 187bp size, 3 One material was amplified to bands of 187bp and 376bp, and the rest of the material was a band of 376bp ( figure 2). Of the 16 materials carrying 187bp bands and 3 materials carrying 187bp and 376bp bands, 11 materials also carried the scab resistance gene Rx4. In order to exclude the influence of this gene on re...

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Abstract

The present invention relates to a molecular marker closely linked to a broad-spectrum resistance gene S1Pub24 of tomato bacterial spots, a specific primer pair used for detecting the molecular marker, a kit comprising the specific primer pair, and a method for identifying the resistance gene of the tomato bacterial spots by using the specific primer pair and a use thereof. The molecular marker isan InDel marker and designated as SlPub-Del, and detects a 189 bp sequence located in a 5'-UTR region of the SlPub24 gene, and a material lacking the sequence exhibits disease resistance to the tomato bacterial spots. An experiment proves that the InDel marked S1Pub-Del is strong in specificity and can be used for molecular marker-assisted selection of the broad-spectrum resistance of the tomatobacterial spots. A disease-resistant individual bacterium strain can be obtained by screening in a seedling period, resistance identification is not needed, and the molecular marker save costs, improves selection efficiency and greatly accelerate breeding processes.

Description

technical field [0001] The invention belongs to the field of vegetable genetics and breeding, and relates to a molecular marker related to broad-spectrum resistance to tomato scab and its application. Background technique [0002] Tomato scab is a bacterial disease caused by multiple species of Xanthomonas spp. As early as the 1940s, the disease occurred in Hunan and Jiangxi areas of my country. It has been reported in all provinces, municipalities and autonomous regions except Tibet. It is one of the four major bacterial diseases in tomato production in my country. had a greater impact on tomato production (Wang et al.2018). Because the pathogenic bacteria of this disease are very complex, there are four species and at least four physiological races (T1-T4) (Jones et al.2004,2005), and the pathogenic bacteria can produce drug resistance, which has brought great challenges to the chemical control of the disease. At the same time, large-scale application of pesticides will c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 杨文才刘欣
Owner CHINA AGRI UNIV
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