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Fusion protein and applications thereof

A technology of fusion protein and fluorescent protein, applied in fusion protein and its application field

Active Publication Date: 2019-06-11
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Soma-axon release occurs in the axon terminal of the posterior pituitary gland, and the released oxytocin can directly enter the peripheral circulatory system and play multiple roles. It is directly related to the secretion of oxytocin in the peripheral system, because some organs in the peripheral system such as: uterus, breast, kidney, liver, heart, etc. also synthesize oxytocin, but its content is far lower than that in the central nervous system

Method used

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  • Fusion protein and applications thereof
  • Fusion protein and applications thereof
  • Fusion protein and applications thereof

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Embodiment Construction

[0026] The technical scheme of the present invention will be further explained below in conjunction with experiments.

[0027] 1. Design of molecular probe

[0028] Design a molecular probe that can track oxytocin and calcinin separately, and the molecular probe can also explore the molecular structure relationship between oxytocin and calcinin

[0029] Through base synthesis of oxytocin and neuropituitary posterior lobe fragments with different reporter genes, oxytocin is linked to the reporter gene yellow fluorescent group through a linking loop (glycine-glycine-glycine-glycine-glycine-valine) The linking ring is rich in glycine because of its simple molecular structure and greater flexibility. It can reduce the interference of the 238 amino acid yellow fluorescent group on the spatial structure of oxytocin with only 9 amino acids; neurohypophysis The leaf carrier protein reporter gene is a blue-green fluorescent group with no connecting ring in the middle. Because the neuropituit...

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Abstract

The invention discloses a molecular probe for tracking oxytocin. A fusion protein comprises oxytocin, a connecting ring, fluorescent protein 1, an enzyme cleavage site, neurophysin and fluorescent protein 2. The molecular probe of the invention can track the oxytocin and the neurophysin, and the molecular probe can also explore the molecular structure relationship between the oxytocin and the neurophysin.

Description

Technical field [0001] The invention relates to a fusion protein and its application. Background technique [0002] Oxytocin is a neuropeptide. It not only promotes uterine contraction during female delivery and subsequent lactation milk secretion, but a series of studies have also found that oxytocin has effects on the digestive system, cardiovascular system, and even learning, memory, and sexual behavior. Significant influence. The latest research also shows that oxytocin can promote maternal and social behaviors in different species, enhance the relationship between people, and promote communication and trust between people; oxytocin is also effective for many mental illnesses such as autism. Schizophrenia, social anxiety disorder, etc. have certain therapeutic functions. In today's society, human beings are faced with these many pressures at all times, so the incidence of these diseases in contemporary society is getting higher and higher, but there is still a lack of effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62
Inventor 吴冈义何湘黔霍清伟
Owner SOUTH CHINA NORMAL UNIVERSITY
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