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Metabolites related to acute pancreatitis and their application

A technology for acute pancreatitis and metabolites, applied to metabolites and their application fields, can solve the problems of difficult application in clinical departments, early diagnosis and treatment of unfavorable diseases, and many variables, and achieves the effect of being conducive to personalized treatment.

Active Publication Date: 2020-03-06
PEOPLES HOSPITAL OF DEYANG CITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many multiple scoring systems for assessing the severity of AP clinically, such as APACHEII, Ransom BISAP, etc., but these evaluation systems are time-consuming and have many variables, so it is difficult to apply to clinical departments other than intensive care departments (Pavlidis TE, Pavlidis ET, Sakantamis AK.Advances in prognostic factors in acute pancreatitis: a mini-review[J].Hepatobiliary Pancreat DisInt.2010,9(5):482-6.), not conducive to early diagnosis and treatment of diseases

Method used

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  • Metabolites related to acute pancreatitis and their application
  • Metabolites related to acute pancreatitis and their application
  • Metabolites related to acute pancreatitis and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 Chinese medicine is to the treatment of acute pancreatitis

[0046] 1. Experimental materials

[0047] 1.1 Experimental main materials

[0048] Table 1 List of materials used

[0049]

[0050] 1.2 The main equipment of the experiment

[0051] Surgical instruments: 2 sterile microscopic curved forceps, 2 ophthalmic scissors, 1 needle forceps, and 1 scalpel.

[0052] Others: dry cotton balls, medical cotton swabs, sutures, surgical lights, surgical boards, fixed rubber bands, alcohol cotton, sterile gauze, sterile petri dishes, normal saline, 10mL syringes, 1mL syringes, No. 4 suture needles.

[0053] 2. Experimental method

[0054] 2.1 Animal grouping

[0055] 30 SPF-grade C57BL / 6 mice, male, 8 weeks old, weighing about 20g, were randomly divided into five groups after one week of adaptive feeding, with 6 mice in each group. The grouping conditions are shown in Table 2:

[0056] Table 2 Grouping of mice

[0057]

[0058] 2.2 Drug preparation

...

Embodiment 2

[0099] Example 2 Screening for metabolites related to acute pancreatitis

[0100] 1. Collection of samples

[0101] In this experiment, 10 mouse tissue samples were collected from each of the blank control group, the model group and the rhein+honokiol combined treatment group in the embodiment.

[0102] 2. Experimental information

[0103] 2.1 Instruments and reagents

[0104] Table 4 Reagents

[0105]

[0106] Table 5 Instruments

[0107]

[0108] 2.2 Experimental method

[0109] 2.2.1 Metabolite extraction

[0110] 1) Take 100mg of tissue and add 500μL of lysate (MeOH:H 2 O=1:1);

[0111] 2) Crushed in a tissue disruptor, 6,500MHz, 1min, oscillating 3 times;

[0112] 3) After vortex mixing, place in a -20°C refrigerator overnight;

[0113] 4) Centrifugation: 4°C, 13,000rpm, 20min;

[0114] 5) take the same volume of supernatant and freeze-dry;

[0115] 6) Add 100 μL of lysate to redissolve, and sonicate for 5 minutes;

[0116] 7) Centrifugation: 4°C, 13,000...

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Abstract

The invention provides an acute pancreatitis related metabolite and an application thereof. The metabolite is hydrochloric acid cinanserin or cefazaflur. The metabolite-hydrochloric acid cinanserin orcefazaflur is adopted for diagnosis of acute pancreatitis and prediction of a response reaction of an acute pancreatitis subject to a therapeutic agent. The invention also provides a pharmaceutical composition for treating the pancreatitis. The pharmaceutical composition can cause the change of the metabolic level.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to metabolites related to acute pancreatitis and applications thereof. Background technique [0002] Acute pancreatitis (AP) is one of the common diseases that cause acute abdominal pain. It is caused by a variety of causes that cause the activation of pancreatic enzymes in the pancreas, causing pancreatic tissue self-digestion, edema, and bleeding, and local inflammation in the pancreas. The main features of the disease, severe disease may occur systemic inflammatory response syndrome (Systemic inflammatory response syndrome, SIRS), accompanied by organ dysfunction (Organ dsfunction, OD). The clinical manifestations of acute pancreatitis are diverse, with low specificity, and the predictive sensitivity for poor prognosis is lower than 40% (SteinbergWM. Predictors of severity of acute pancreatitis [J]. Gastroenterol C1inN Am, 1990,19 (4): 849 -61.), and the pathological basis of systemic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/53G01N33/577G01N30/02G01N21/25G01N21/31G01N21/33G01N21/35G01N21/64G01N21/76G01N27/62G01N27/64
Inventor 黄卫李雨刘航黄承军
Owner PEOPLES HOSPITAL OF DEYANG CITY
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