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Therefore, the artificial blood vessels manufactured by these two methods have disordered internal vascular cells, and it is difficult to form a complete endothelial cell layer and a regular smooth muscle cell layer, so they are still unable to be applied clinically.
Method used
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preparation example Construction
[0512] (1) prepare a tubular biological construct by the method for preparing a tubular biological construct in any one of method 1 or method 2;
[0513] Or, prepare the sheet-like biological construct by the method for preparing the sheet-like biological construct in method 3; afterward, bend the prepared sheet-like biological construct, and / or make the edge of the sheet-like biological construct gluing, resulting in tubular biological constructs;
[0514] (II) provide materials (such as biocompatible materials) for preparing solid supports, and prepare tubular solid supports on the outer wall of the tubular biological construct;
[0515] In certain preferred embodiments, the tubular solid support is prepared by 3D printing or spraying.
[0516] The method for preparing an artificial tissue precursor as defined in any one of methods 1-6, further comprising shaping the biological construct.
[0517] In some preferred embodiments, according to the structural stability and thi...
specific Embodiment approach
[0679] The invention will now be described with reference to the following examples, which are intended to illustrate the invention, but not to limit it.
[0680] Reagents, kits or instruments whose sources are not specified in the examples are all conventional products commercially available on the market. Those skilled in the art understand that the examples describe the present invention by way of example and are not intended to limit the scope of the claimed invention.
Embodiment 1
[0681] Example 1 Preparation of biobricks comprising rhesus monkey adipose-derived mesenchymal stem cells
[0682] 1. Collection and culture of adipose-derived mesenchymal stem cells
[0683] (1) Collection of adipose-derived mesenchymal stem cells: Using rhesus monkey as an animal model, adipose tissue was cut from the groin and placed in a 50mL centrifuge tube; the adipose tissue was digested with trypsin, and adipose-derived mesenchymal stem cells were collected by centrifugation.
[0684] (2) Adipose-derived mesenchymal stem cells were expanded and cultured using serum-free Lonza medium. Micrographs of the cells of the fourth passage in the primary culture are as follows: Figure 6 shown. It can be seen from the figure that the cells are uniform in shape and in good growth state. Cells were harvested by centrifugation.
[0685] 2. Preparation of collagen solution
[0686] Formulated using bovine type I collagen.
[0687] (1) High temperature sterilization treatment o...
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Abstract
The invention relates to the field of tissue engineering and 3D printing, in particular to an artificial tissue precursor and a method for preparing the same. The artificial tissue precursor comprisesa solid support and a plurality of microcapsules, wherein at least one microcapsule is attached to the solid support, and each microcapsule comprises a cell and a biocompatible material encapsulatingthe cell. The method for preparing the artificial tissue precursor and a kit and a kit set for preparing the artificial tissue precursor are further provided. The artificial tissue is cultured by theartificial tissue precursor, like an artificial lumen. The artificial tissue precursor or a luminal implant or a luminal model of the artificial lumen is included. The artificial tissue precursor isused for preparing the artificial tissue, the luminal implant or the luminal model; the artificial tissue is used for preparing the luminal implant or the luminal model.
Description
[0001] This application is a divisional application of the international application PCT / CN2017 / 101738, which entered the Chinese national phase on March 29, 2018, with the application number 201780003356.2, and the title of the invention is "artificial tissue precursor and its preparation method". technical field [0002] The invention relates to the fields of tissue engineering and 3D printing. In particular, the present invention relates to an artificial tissue precursor comprising a solid support and a plurality of microcapsules, wherein at least one microcapsule is attached to the solid support, said microcapsules comprising cells and a biological phase surrounding the cells Capacitive materials; methods for preparing said artificial tissue precursors; kits and kits for preparing artificial tissue precursors; artificial tissues cultured from said artificial tissue precursors, such as artificial lumens; comprising said artificial Lumenal implants or luminal models of tissu...
Claims
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