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Bacteria-carrying nano-chitosan livestock house disinfectant and its preparation method and application

A technology of nano-chitosan and disinfectant, which is applied in the direction of disinfectant, botany equipment and methods, application, etc., can solve the problems that have not been reported, achieve the optimization of antibacterial and antibacterial effect, improve the production performance of livestock and poultry, and prolong the effect the effect of time

Active Publication Date: 2021-02-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, chitosan and Bacillus subtilis are generally used as feed additives in the breeding industry, and enter the livestock and poultry body through the digestive tract to play a role, but the compound of chitosan nanoparticles and Bacillus subtilis is used as a disinfectant for livestock houses. Not yet reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, a kind of preparation method of the nano-chitosan livestock house disinfectant (carrying bacteria nano-chitosan livestock house disinfectant) of carrying Bacillus subtilis, carries out the following steps successively:

[0024] 1), preparation mass concentration is the nano-chitosan suspension of 2%:

[0025] It is prepared according to Example 8 of the invention of Patent No. 201510417163.4 "A Preparation Method and Application of Chitosan Nanoparticle Suspension".

[0026] 2), heating the Bacillus subtilis in a water bath to 60°C, quickly adding xanthan gum, and mixing evenly, so that the xanthan gum is coated on the surface of the Bacillus subtilis to form a Bacillus subtilis complex;

[0027] The dosage ratio of xanthan gum and Bacillus subtilis is 1:1;

[0028] 3), under the stirring condition of an electric mixer with digital display speed, slowly add the Bacillus subtilis complex obtained in step 2) (the addition time is 5 minutes) to the chitosan ...

Embodiment 2

[0034] Embodiment 2, a kind of preparation method of carrying the nano-chitosan livestock house disinfectant of Bacillus subtilis, carries out following steps successively:

[0035] 1), preparation mass concentration is the nano-chitosan suspension of 8%:

[0036] It is prepared according to Example 5 of the invention of Patent No. 201510417163.4 "A Preparation Method and Application of Chitosan Nanoparticle Suspension".

[0037] 2), heating the Bacillus subtilis in a water bath to 60°C, quickly adding xanthan gum, and mixing evenly, so that the xanthan gum is coated on the surface of the Bacillus subtilis to form a Bacillus subtilis complex;

[0038] The dosage ratio of xanthan gum and Bacillus subtilis is 2:1;

[0039] 3), under the stirring condition of an electric mixer with digital display speed, slowly add the Bacillus subtilis complex obtained in step 2) (the addition time is 10 minutes) to the chitosan nanoparticle suspension, chitosan and Bacillus subtilis The mass ...

Embodiment 3

[0044] Embodiment 3, a kind of preparation method of carrying the nano-chitosan livestock house disinfectant of Bacillus subtilis, carries out following steps successively:

[0045] 1), preparation mass concentration is the nano-chitosan suspension of 5%:

[0046] It is prepared according to Example 4 of the invention of Patent No. 201510417163.4 "A Preparation Method and Application of Chitosan Nanoparticle Suspension".

[0047] 2), heating the Bacillus subtilis in a water bath to 60°C, quickly adding xanthan gum, and mixing evenly, so that the xanthan gum is coated on the surface of the Bacillus subtilis to form a Bacillus subtilis complex;

[0048] The dosage ratio of xanthan gum and Bacillus subtilis is 1.5:1;

[0049] 3), under the stirring condition of an electric mixer with digital display speed, slowly add the Bacillus subtilis complex obtained in step 2) (the addition time is 8 minutes) to the chitosan nanoparticle suspension, chitosan and Bacillus subtilis The mass...

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PUM

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Abstract

The invention discloses a bacterium-loaded nano chitosan animal house disinfectant which is prepared by mixing chitosan and bacillus subtilis at a mass ratio of (2-4):1. The invention further providesa preparation method of the bacterium-loaded nano chitosan animal house disinfectant at the same time. The preparation method comprises the following steps: heating the bacillus subtilis in a water bath, then adding xanthan gum for uniform mixing, slowly adding an obtained bacillus subtilis complex into a chitosan nanoparticle suspension, continuing stirring after addition, drying the obtained bacillus subtilis-loaded nano chitosan suspension till a water content is 5-10%, and obtaining the bacterium-loaded nano chitosan animal house disinfectant. After dilution, the bacterium-loaded nano chitosan animal house disinfectant is sprayed into an animal house, can achieve a continuous and stable antibacterial effect, and improves air quality of the animal house and livestock productivity.

Description

technical field [0001] The invention belongs to the technical field of livestock house disinfectants, and in particular relates to a nano-chitosan livestock house disinfectant containing Bacillus subtilis and a preparation method thereof. Background technique [0002] Disinfection is an important and necessary part of the farm. Doing a good job in daily disinfection is one of the important measures to prevent the infection of the farm and control the outbreak of the disease, and it is an important guarantee for the efficient development of the farm. Common disinfectants in the aquaculture industry are mainly divided into two categories: organic and inorganic disinfectants, which have disadvantages such as volatile, easy to decompose, poor stability, short action time, and may decompose to produce harmful substances, strong corrosion, and release irritating gases. The health of livestock and poultry will be adversely affected, so it is very important to develop new healthy an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/22A01N43/16A01P1/00
Inventor 韩新燕姜雪梅曹沛文
Owner ZHEJIANG UNIV