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A method for obtaining Cordyceps sinensis from host insect bat moth larvae infected by Trichosporum in China

A technology of bat moth larvae, Trichospora chinensis, applied in the fields of botanical equipment and methods, horticulture, agriculture, etc., can solve the problems of shortening the infection rigidity time, low mortality rate, large wound on the surface of the parasite, and shortening the infection rigidity. effect of time, low mortality

Active Publication Date: 2021-06-15
INNER MONGOLIA FURUI MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the body surface coating and feeding methods take a long time, the infected larvae have a high mortality rate, and the infection rate is low; the existing puncture infection method is prone to excessive wounds on the surface of the worm body and excessive loss of larvae body fluids due to careless operation And the death rate is high
[0007] Therefore, the existing technology cannot simultaneously achieve the effects of low mortality, high infection rate and shortening the ossified time of infection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The bat moth used in this example is Thitarodes gonggaensis collected from Sichuan.

[0023] 1. Preparation of Chinese hairy sporozoites

[0024] The medium for cultivating blastospores is PDA medium: each liter contains 20g of glucose, 200g of potato, 5g of peptone, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g and VB 1 0.02g, the rest is H 2 O (natural pH). After accurate molecular identification, the conidia of Trichosporum sinensis were inserted into the above-mentioned PDA medium, cultured in a shaker (120rpm, 9°C) for 60 days, and the blastospores of Trichosphaera sinensis were collected by centrifugation for injection to infect bat moths larva.

[0025] 2. Breeding of bat moth larvae

[0026] The larvae of the bat moth were mixed and fed with carrots, Polygonum villosa and Potentilla velvet in a mass ratio of 1:1:1 to obtain healthy 5th instar bat moth larvae for infection.

[0027] 3. Injection infection and post-injection care

[0028] Inject the 5th instar bat...

Embodiment 2

[0035] The bat moth used in this example is Thitarodes gonggaensis collected from Sichuan.

[0036] 1. Preparation of Chinese hairy sporozoites

[0037] The medium for cultivating blastospores is PDA medium: each liter contains 20g of glucose, 200g of potato, 5g of peptone, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g and VB 1 0.02g, the rest is H 2 O (natural pH). After accurate molecular identification, the conidia of Trichosporum sinensis were inserted into the above-mentioned PDA medium, cultured in a shaker (120rpm, 9°C) for 60 days, and the blastospores of Trichosphaera sinensis were collected by centrifugation for injection to infect bat moths larva.

[0038] 2. Breeding of bat moth larvae

[0039]The larvae of the bat moth were mixed and fed with carrots, Polygonum villosa and Potentilla velvet in a mass ratio of 1:1:1 to obtain healthy 5th instar bat moth larvae for infection.

[0040] 3. Injection infection and post-injection care

[0041] Inject the 5th instar bat ...

Embodiment 3

[0048] The bat moth used in this example is Thitarodes gonggaensis collected from Sichuan.

[0049] 1. Preparation of Chinese hairy sporozoites

[0050] The medium for cultivating blastospores is PDA medium: each liter contains 20g of glucose, 200g of potato, 5g of peptone, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g and VB 1 0.02g, the rest is H 2 O (natural pH). After accurate molecular identification, the conidia of Trichosporum sinensis were inserted into the above-mentioned PDA medium, cultured in a shaker (120rpm, 9°C) for 60 days, and the blastospores of Trichosphaera sinensis were collected by centrifugation for injection to infect bat moths larva.

[0051] 2. Breeding of bat moth larvae

[0052] The larvae of the bat moth were fed with carrots, Polygonum villosa and Potentilla velvet in a mass ratio of 1:1:1 to obtain healthy, infected 2nd instar larvae of the bat moth.

[0053] 3. Injection infection and post-injection care

[0054] The 2nd instar bat moth larvae i...

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Abstract

The invention discloses a method for obtaining Cordyceps sinensis from the larvae of the host insect bat moth infected by Trichosporum in China. The present invention improves the three key steps of the spore form (blastospore) of the injected Mortierella sinensis, the injection method (operated under sterile conditions) and the post-injection care (at a low temperature of 0-6°C), so that the injected Infecting bat moth larvae achieves low mortality (less than 30%), high infection rate (both can reach 100% bacteria-carrying rate) and shortens the infection ossification time (3-5 months), and obtains the same morphology as wild Cordyceps sinensis The artificial ecological tending of Cordyceps sinensis.

Description

Technical field: [0001] The invention belongs to the field of artificial cultivation of Cordyceps sinensis, and in particular relates to a method for obtaining Cordyceps sinensis from larvae of host insects bat moths infected by Trichosporum in China. Background technique: [0002] Cordyceps sinensis is the most distinctive biological resource in my country, belonging to Ascomycota, Sordariomycetes, Hypocreales, Ophiocordycipitaceae, and Ophiocordyceps. In Tibet, Qinghai, Yunnan, Sichuan, Gansu and other snow-capped alpine regions above 3,000 meters above sea level. [0003] Cordyceps sinensis is the larvae of host insects-bat moths infected by the fungus Ophiocordyceps sinensis in China, and makes them ossified. Under suitable conditions, the fungus scorpion grows and forms a composite form of worm (zombie) grass (fungi fruiting body). structure. [0004] Cordyceps sinensis has many functions such as "tonifying the lungs, strengthening the kidneys, benefiting essence and q...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00
CPCA01G18/00
Inventor 曹莉刘桂清吴华韩日畴
Owner INNER MONGOLIA FURUI MEDICAL SCI
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