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Method for preparing slices of black tiger root

A technology of black tiger root and slicing, which is applied in the field of paraffin slice preparation, can solve the problem of not using paraffin slices, etc., and achieves the effects of convenient microscopic observation and identification, convenient source, and promotion of full contact.

Active Publication Date: 2021-10-15
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ultrasound is not used in the softening of paraffin sections

Method used

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  • Method for preparing slices of black tiger root
  • Method for preparing slices of black tiger root
  • Method for preparing slices of black tiger root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for preparing slices of black tiger root, comprising the following steps:

[0040] Step 1: Prepare softener: Measure 5 parts of potassium hydroxide solution with a mass concentration of 50%, add 3 parts of glycerol with a volume fraction of 30%, and 2 parts of ultrapure water, and stir until completely dissolved to obtain a softener.

[0041] Step 2: Adjust the temperature of the ultrasonic instrument to 60°C, soak the dried root of black tiger in softener and transfer to the ultrasonic instrument, perform ultrasonic treatment at 80Hz for 5 times, each time for 30 minutes, and obtain softened black tiger root. Tiger root.

[0042] Step 3: Preparation of fixative solution: 5 parts of formalin solution, 5 parts of n-propionic acid or glacial acetic acid solution, and 90 parts of alcohol with a volume concentration of 70% were mixed to obtain a fixative solution.

[0043] Step 4: Cut the softened black tiger root obtained in step 2 into small pieces of 5 mm, put ...

Embodiment 2

[0051] Step 1 of Example 1 is replaced by: measure 5 parts of sodium hydroxide solution with a mass concentration of 50%, add 3 parts of glycerol and 2 parts of ultrapure water with a volume fraction of 30%, stir until completely dissolved, and obtain softener. All the other parameters are exactly the same as Example 1, and the preparation method is also exactly the same.

Embodiment 3

[0053] Replace step 2 of Example 1 with: adjust the temperature of the ultrasonic instrument to 50°C, soak the dried black tiger root in softener and transfer it to the ultrasonic instrument, and perform ultrasonic treatment 5 times under the condition of 80Hz, each time In 30 minutes, the softened black tiger root was obtained. All the other parameters are exactly the same as Example 1, and the preparation method is also exactly the same.

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Abstract

The invention discloses a method for preparing black tiger root slices, which uses the black tiger root as a raw material, soaks in a softener and adopts ultrasonic treatment to obtain the slices of the black tiger root. Wherein, the softening agent includes the following raw materials in parts by weight: 5 parts of potassium hydroxide or sodium hydroxide solution with a concentration of 50%, 3 parts of glycerol with a volume fraction of 30%, and 2 parts of ultrapure water. The softener prepared from strong alkali, glycerin and water can not only quickly and effectively soften the roots of the black tiger, but also has simple raw materials, convenient sources, and low toxicity. Combined with ultrasonic heating technology, the softening process is more effective. Sufficient, making the prepared slices better.

Description

technical field [0001] The invention belongs to the technical field of preparation of paraffin wax slices, and in particular relates to a method for preparing black tiger root slices. Background technique [0002] The black tiger Kadsura coccinea var.coccinea is a perennial evergreen vine of the genus Kadsura of the genus Schisandra in the family Magnoliaceae. It is used as medicine with its dried roots. It is one of the traditional Zhuang Yao medicinal materials commonly used by ethnic minorities in Guangxi. Rheumatic bone pain, bruises and other biological activities are mainly distributed in black tigers in Jiangxi, Hunan, Guangdong and Hong Kong, Hainan, Guangxi, Sichuan, Guizhou, Yunnan and other places in China. Due to the intensified market demand in recent years, coupled with the slow renewal of wild black tiger resources, the market price of black tigers has been rising year by year, and there are often confused products among genuine medicinal materials. Therefore,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
CPCG01N1/28G01N1/30
Inventor 周雅琴余丽莹谢月英农东新黄宝优王春丽
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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