Application of dandelion sterol in the preparation of drugs for the treatment of nicotine poisoning

A technology of dandelion sterol and nicotine, which is applied in the field of medicine, can solve the problems of unreported research and application, and achieve the effect of no toxic side effects, significant drug effect and long duration

Active Publication Date: 2022-03-15
ZAOZHUANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dandelion sterols are widely used in the field of medicine. It is reported that dandelion sterols have antibacterial and anti-inflammatory effects, but there is no report on its application in the treatment of nicotine poisoning

Method used

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  • Application of dandelion sterol in the preparation of drugs for the treatment of nicotine poisoning
  • Application of dandelion sterol in the preparation of drugs for the treatment of nicotine poisoning

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Extraction process of dandelion sterols

[0029] 1 In the early morning of summer, pick fresh lettuce plants that have been identified by experts, and cut them into small sections with scissors;

[0030] 2 Absorb lettuce milk with clean analytical filter paper;

[0031] 3. Put the analysis filter paper saturated with the milk of lettuce into the vacuum freeze dryer, and freeze-dry to get the milk powder;

[0032] 4. Extract the lettuce milk powder with 90% ethanol solution overnight to obtain paste extract A;

[0033] 5. Extract A with petroleum ether, and distill under reduced pressure to obtain extract B;

[0034] 6. Elute B with 50:1 petroleum ether-acetone solution to obtain eluate C;

[0035] 7. Separate C by silica gel chromatography, and then use 10:1 petroleum ether-ethyl acetate to obtain eluate D; dry.

[0036] 8. Finally, D is analyzed and identified by high-performance liquid chromatography, and it is proved that D is dandelion sterol, and its purity is ...

Embodiment 2

[0038] There are 260 Kunming mice, half male and half male. Level 1, weighing (18±2) g, purchased from the College of Life Sciences, Shandong University.

[0039] 1. Selection of lethal dose of nicotine in mice

[0040] 80 mice, half male and half male, were randomly divided into 4 groups, 20 mice in each group. Nicotine is soluble in saline. Because the mass of each mouse does not exceed 20 g, in order to control the volume of the injection within 1 ml, we set the concentrations of nicotine solutions at 500 μg / ml, 1000 μg / ml and 1500 μg / ml, respectively. Each mouse in the first group, the second group and the third group was subcutaneously injected with 25 μg / g, 50 μg / g and 75 μg / g of nicotine, and each mouse in the control group was injected with 1 ml of normal saline. Calculate the average time and standard deviation of each group of mice showing nicotine poisoning symptoms ( ±s , n =20) and the number of dead mice in each group was recorded.

[0041]

[0042] Afte...

Embodiment 3

[0044] 2. Dandelion sterol anti-nicotine test

[0045] 120 mice, half male and half male, were randomly divided into 6 groups, 20 mice in each group. Dandelion sterols are dissolved in normal saline, the concentrations are 500μg / ml, 400μg / ml, 300μg / ml, 200μg / ml, 100μg / ml respectively. First, the first group, the second group, the third group, the fourth group and the fifth group, each mouse was injected with dandelion at doses of 25 μg / g, 20 μg / g, 15 μg / g, 10 μg / g and 5 μg / g Sterol, the control group was injected with 1ml of normal saline, and then the first, second, third, fourth, fifth and control groups were given subcutaneous injections of nicotine at a dose of 75 μg / g to each mouse. Calculate the average time and standard deviation ( ±s , n =20) and the number of dead mice in each group was recorded.

[0046] Table 2 Dandelion sterol anti-nicotine experimental statistical table ( ±s , n =20)

[0047]

[0048] When a dose of 75 μg / g nicotine was injected into mic...

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Abstract

The invention belongs to the field of medicine, and in particular relates to the application of dandelion sterol in the preparation of medicine for treating nicotine poisoning. The medicine for treating nicotine poisoning prepared by the described dandelion sterol comprises the following components: 10 parts of dandelion sterol, 15 parts of compressible starch, 10 parts of microcrystalline cellulose, 15 parts of dextrin, 10 parts of sucrose, 10 parts of mannitol 10 parts, 10 parts of menthol, 15-20 parts of lactose, 0-5 parts of magnesium stearate. Using plant extraction, the extracted substances are of high purity and have no toxic or side effects. The tablet prepared by the invention can effectively treat nicotine poisoning and improve the stimulation and damage of nicotine to many organs of human body due to remarkable drug effect and long duration. The prepared tablets are extracted from plants and are low in price.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of dandelion sterol in the preparation of medicine for treating nicotine poisoning. Background technique [0002] According to reports, more than 2,000 people die from smoking-related diseases every day in the country, and it is estimated that the number of deaths will increase to 8,000 by 2050. Today, when the birth rate is declining, the aging population is increasing, and the demographic dividend is gradually disappearing, tobacco control is an urgent task. In China, although many cities have banned smoking in public places, due to historical reasons and the influence of social atmosphere, smoking has already become a fashion. Not only will the smoking ban not be achieved overnight, but there is also a tendency for smokers to become younger and more feminine. Therefore, it is urgent to develop a drug capable of degrading the toxicity of nicotine. [0003]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/56A61K9/20A61P39/02C07J63/00
CPCA61K31/56A61K9/2059A61K9/0056A61P39/02C07J63/008
Inventor 盛积贵李晓梅张琳
Owner ZAOZHUANG UNIV
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