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Sample concentration device and method for capillary electrophoresis

A technology of capillary electrophoresis and concentration device, which is applied in the direction of measuring device, test sample preparation, sampling, etc. It can solve the problems that samples cannot be completely separated, limited, and cannot be exceeded, so as to achieve easy assembly and use, improve detection limit, The effect of simple structure

Pending Publication Date: 2019-07-23
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the higher injection volume will cause the sample to be incompletely separated, and the injection volume of these existing sample concentration methods cannot exceed 1 capillary column volume, so the injection volume of capillary electrophoresis is still limited compared with liquid chromatography

Method used

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  • Sample concentration device and method for capillary electrophoresis
  • Sample concentration device and method for capillary electrophoresis
  • Sample concentration device and method for capillary electrophoresis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Such as figure 1 As shown, a sample concentration device for capillary electrophoresis includes a conductive connection chamber 1, an electrophoresis unit 2, at least one sample injection unit 3, a first high-voltage power supply 4 and a second high-voltage power supply 5, and the connection chamber 1 is made of a conductive material Made, the connecting chamber 1 is electrically connected with the first high-voltage power supply 4, the connecting chamber 1 communicates with one end of the sampling unit 3 and one end of the electrophoresis unit 2 respectively, and the other end of the sampling unit 3 is connected with the second high-voltage power supply 5 Electrically connected, the electrophoresis unit 2 is connected with a detector 6, the other end of the electrophoresis unit 2 is grounded, between the connection chamber 1 and the sampling unit 3, and between the connection chamber 1 and the electrophoresis unit 2 are provided to ensure airtightness. airtight mechani...

Embodiment 2

[0031] Such as figure 2 As shown, a sample concentrating device for capillary electrophoresis, the rest of the structure is the same as that of Embodiment 1, the difference is that in this embodiment, three sampling units 3 are included, and the connection chamber 1 consists of a metal cross 10 The middle part is composed of the first end of the metal cross 10 including a first opening 11, the first opening 11 communicates with one end of the first capillary 21, the second end, the third end and the fourth end of the metal cross 10 The ends are provided with second openings 12 respectively, and the three second openings 12 communicate with the three second capillaries 31 respectively, and the other ends of the three second capillaries 31 are respectively inserted into the holding liquids of the three second sample bottles. The second sample bottle can be the second sample bottle 33 (for sample injection) containing the sample solution or the second sample bottle 32 (for sampl...

Embodiment 3

[0034] A sample concentration method of capillary electrophoresis of the present invention comprises the following steps:

[0035] 1) Sampling: The sample solution is injected by gas pressure to fill the electrophoresis unit and all sampling units;

[0036] 2) Sample concentration: load all sampling units with background electrolyte, apply different voltages to the first high-voltage power supply and the second high-voltage power supply, so that the lowest potential in the connection chamber is lower than that of other units, and the sample ions are concentrated in the connection chamber , after waiting for a long enough time, the sample ions in the electrophoresis unit and all the sampling units are concentrated into the connecting chamber, where the sample ions are positive ions;

[0037] 3) Sample separation: Apply a high voltage with a positive polarity to both the first high-voltage power supply and the second high-voltage power supply, so that the concentrated sample ion...

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Abstract

The invention discloses a sample concentration device and method for capillary electrophoresis. The sample concentration device is characterized by comprising a connecting cavity, an electrophoresis unit, at least one sample introduction unit, a first high voltage power supply and a second high voltage power supply, the connecting cavity adopts a conductive material, the connecting cavity is electrically connected with the first high voltage power supply, the connecting cavity is respectively communicated with one end of the sample introduction unit and one end of the electrophoresis unit, theother end of the sample introduction unit is electrically connected with the second high voltage power supply, the electrophoresis unit is connected with a detector, the other end of the electrophoresis unit is grounded, and an airtight mechanism is arranged between the connecting cavity and the sample introduction unit and between the connecting cavity and the electrophoresis unit respectively.The sample concentration device and method which are disclosed by the invention have the advantages that a low potential is formed at a connection joint, so that sample ions are concentrated towards the connection joint, sample introduction volume of a capillary can be increased to volume of multiple capillary columns, and the sample introduction volume and a detection limit of the capillary electrophoresis are greatly increased.

Description

technical field [0001] The invention relates to the field of capillary electrophoresis, in particular to a capillary electrophoresis sample concentration device and method. Background technique [0002] Capillary electrophoresis has the advantages of high separation efficiency, fast separation speed, and the ability to separate isomers. It has been widely used in many fields such as proteomics, metabolomics, drug analysis, and food testing. [0003] Injection volume is one of the main reasons that limit the further widespread application of capillary electrophoresis compared to liquid chromatography. The injection volume of traditional capillary zone electrophoresis is only about 1% of the volume of the capillary column. According to the calculation of a typical capillary with an inner diameter of 30 microns and a length of 1 meter, the injection volume of the sample is only 7 nanoliters. Increasing the inner diameter of the capillary or increasing the length of the capilla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/40G01N27/447
CPCG01N1/40G01N27/447G01N2001/4038
Inventor 唐科奇吴焕铭
Owner NINGBO UNIV
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