Application of Anemarrhena polysaccharide in preparation of medicine for treating dry eye disease

A technology for dry eye disease and Anemarrhena, applied in the field of medicines containing polysaccharides from plants of the genus Anemarrhena, can solve the problems of slow onset of action, difficult to popularize and apply uniformly among people with different constitutions, etc., and achieves the reduction of lacrimal gland lesions and the recovery of the number of goblet cells. , the effect of increased lacrimal secretion

Active Publication Date: 2021-07-27
西施兰(珠海)医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the onset of oral compound traditional Chinese medicine is slow, and it is difficult to uniformly promote and apply it to people with different constitutions

Method used

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  • Application of Anemarrhena polysaccharide in preparation of medicine for treating dry eye disease
  • Application of Anemarrhena polysaccharide in preparation of medicine for treating dry eye disease
  • Application of Anemarrhena polysaccharide in preparation of medicine for treating dry eye disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Preparation of Anemarrhena polysaccharide by microwave extraction method

[0021] (1) Take 2 kg of Anemarrhena, dry it and crush it to a coarse powder, add 10 times and 8 times the volume of water as a solvent in turn, and use a 1KW-20KW microwave extraction device to extract twice, each time for 5-30 minutes, and combine twice The extract was concentrated under reduced pressure to 20L, and was pre-filtered by a three-legged centrifuge, and the supernatant filtrate was taken, and potassium sorbate with a final concentration of 0.3% was added for preservation;

[0022] (2) the filtrate is decolorized and deproteinized by cationic resin, the eluted filtrate is collected, the pH is adjusted to neutral, and the mother extract is obtained by vacuum concentration;

[0023] (3) Put the extract on a DEAE-cellulose column, wash the column with NaCl of different concentrations from 0M to 1M, then detect the protein with the Coomassie Brilliant Blue method, and discard the elua...

Embodiment 2

[0028] 1. Extract and prepare Anemarrhena polysaccharide by conventional water extraction method

[0029] (1) Take 2kg of Anemarrhena, dry it and crush it to a coarse powder, add 10 times and 8 times of water in turn, decoct twice, each time for 1 hour; combine the decoctions from the two times, concentrate under reduced pressure, and concentrate to 20L, three-legged Centrifuge primary filtration, take the supernatant filtrate, and add potassium sorbate to a final concentration of 0.3% for preservation;

[0030] Steps (2) to (4) are the same as in Example 1

[0031] 2. Identification of polysaccharide content and molecular weight

[0032] Using the same method as in Example 1, the content of the Anemarrhena polysaccharide obtained in this example is 90%, and the molecular weight of the Anemarrhena polysaccharide is 200 Da-2,000,000 Da.

Embodiment 3

[0034] 1. Preparation of Anemarrhena polysaccharide by ultrasonic extraction

[0035] (1) Take 2 kg of Anemarrhena, dry it and crush it to a coarse powder, add 10 times and 8 times of water in turn, extract twice with an ultrasonic extractor, each time for 5 to 30 minutes, combine the decoctions of the two times, and concentrate under reduced pressure , concentrated to 20L, pre-filtered by a three-legged centrifuge, and the supernatant filtrate was added to preserve it by adding 0.3% potassium sorbate.

[0036] (2) Steps (2) to (4) are the same as in Example 1.

[0037] 2. Identification of polysaccharide content and molecular weight

[0038] Using the same method as in Example 1, the polysaccharide content of the freeze-dried powder obtained in this example was measured to be 30.21%, and the molecular weight was 200 Da to 2 million Da.

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Abstract

The present invention relates to the new application of Anemarrhena polysaccharide, in particular to the application of Anemarrhena polysaccharide in the preparation of medicine for treating dry eye disease, the application described in the present invention. The medicine described in this application is an external preparation made of Anemarrhena polysaccharide and conventional dosage of medically acceptable excipients, such as eye drops and eye gel. The medicine described in the invention has the advantages of being colorless, tasteless and non-irritating to the ocular surface mucous membrane.

Description

technical field [0001] The present invention relates to medical preparations, in particular to medicines containing polysaccharides from plants of the genus Anemarrhena. Background technique [0002] Anemarrhena anemarrhena is the dry rhizome of Liliaceae plant Anemarrhena, which has the effects of nourishing yin and reducing fire, moistening dryness and smoothing intestines, and facilitating defecation. Anemarrhena anemarrhena has a high content of polysaccharides, which is one of its main active ingredients. Pharmacological studies have shown that Anemarrhena polysaccharide has biological activities such as anti-tumor, lowering blood fat, lowering blood sugar, anti-inflammation, and anti-platelet aggregation. At present, Anemarrhena polysaccharide is gradually becoming a hot spot in pharmacological research and development of new drugs. [0003] Dry eye disease is a common and frequently-occurring disease in ophthalmology, which is caused by tear film instability and ocul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P27/02
CPCA61K9/0048A61K31/715A61P27/02
Inventor 王剑
Owner 西施兰(珠海)医药科技有限公司
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