Application of sesquiterpenoids in the preparation of anti-asthma drugs

A technology of sesquiterpene and ester compounds, a new application in the preparation of anti-asthma drugs, the field of sesquiterpene lactone compounds

Active Publication Date: 2018-04-27
泊诺(天津)创新医药研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention relates to the anti-inflammatory mechanism of sesquiterpene compound OOABL in blocking the synthesis and degranulation of leukotrienes in mast cells and the anti-asthma effect and efficacy of effectively alleviating airway obstruction in animal models of asthma. see related reports

Method used

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  • Application of sesquiterpenoids in the preparation of anti-asthma drugs
  • Application of sesquiterpenoids in the preparation of anti-asthma drugs
  • Application of sesquiterpenoids in the preparation of anti-asthma drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1 Molecular and cellular level study of multiple anti-asthma mechanisms of OOABL in BMMCs and human lung cancer A549

[0038] 1. Experimental method

[0039] 1.1. Preparation of BMMCs

[0040] Bone marrow stem cells were taken from the thighs of 6-week male Balb / C mice without the influence of specific pathogens, and the cells were suspended in RPMI1640 medium (including 100U / ml penicillin, 100g / ml streptomycin and 10% fetal bovine serum) and Mature BMMCs were obtained by culturing in the presence of IL-3 (20% commercial mitogen-stimulated spleen cell conditioned medium, pokeweed mitogen-stimulated spleen condition medium, PWM-SCM) for more than 3 weeks. This method has determined that >98% of the cells are BMMCs.

[0041] 1.2 Preparation of PWM-SCM

[0042] Take out the spleen from 6-week-old male Balb / C mice without the influence of specific pathogens, grind the spleen into a cell suspension, pass through a 200-mesh stainless steel sieve, adjust the cell co...

Embodiment 2

[0080] Example 2 Effect and mechanism of OOABL on OVA-induced mouse asthma model

[0081] 1. Experimental method

[0082] 1.1 Grouping and Modeling

[0083] 6-week-old female Balb / C mice without the influence of specific pathogens, weighing 18-22 g, were randomly divided into 4 groups: normal control group; asthma model group; OOABL administration group, Montelukast (Montelukast, Mont) administration group (montelukast is a therapeutic drug for asthma, as a positive control group), and the dexamethasone (Dex) administration group (dexamethasone is an anti-inflammatory drug, as a positive control group). The normal control group did not give any stimulation. The mice in the asthma model group were intraperitoneally injected with antigen sensitizers on days 0, 7, and 14. The specific method was to mix 0.1 mg / ml OVA normal saline with the adjuvant (adjuvant) Alum (alum) at a volume ratio of 1:1. Inject 0.2ml intraperitoneally after uniformity, and inhale with 1% OVA physiologi...

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Abstract

The invention relates to the technical field of biomedical engineering, in particular to application of sesquiterpenoids to preparation of an anti-asthmatic drug. The sesquiterpenoids are extracted from inula flowers. The sesquiterpenoids OOABL have the effects of blocking synthesis of leukotrienes in mastocytes, resisting inflammations in degranulation, effectively relieving airway obstruction in an animal model of asthma and other anti-asthmatic effects.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to the new application of the sesquiterpene lactone compound OOABL isolated from the extract of Inula inula in traditional Chinese medicine in the preparation of anti-asthma medicine. Background technique [0002] Bronchial asthma (referred to as asthma) is a chronic airway inflammation involving eosinophils, mast cells, T cells, and white blood cells and other cells and cell components. On the basis of chronic inflammation, it is accompanied by airway hyperresponsiveness, AHR) and airway remodeling, the essence of which is chronic airway inflammation. Asthma is a world-recognized medical problem, listed by the World Health Organization as one of the four major chronic diseases. Over the past ten years, the morbidity and mortality of asthma in many countries and regions in the world have been on the rise. This phenomenon has attracted the attention of the World Healt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/365A61P11/06C07D307/83
CPCA61K31/365C07D307/83
Inventor 金美花段宏泉秦楠唐生安陈曦
Owner 泊诺(天津)创新医药研究有限公司
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