Brown algae extract and application thereof in inhibiting multiplication of highly-toxic karlodinum in expanding propagation of bait microalgae

A technology of bait microalgae and extract, which is applied in application, animal feed, animal feed, etc., to achieve the effect of preventing invasion, increasing the probability of success, and reducing the risk of food chain transmission

Active Publication Date: 2019-07-30
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Brown algae extract and application thereof in inhibiting multiplication of highly-toxic karlodinum in expanding propagation of bait microalgae
  • Brown algae extract and application thereof in inhibiting multiplication of highly-toxic karlodinum in expanding propagation of bait microalgae
  • Brown algae extract and application thereof in inhibiting multiplication of highly-toxic karlodinum in expanding propagation of bait microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the preparation of brown algae extract:

[0023] Wash fresh copper algae 3 times with seawater to remove surface impurities, then wash 2 times with distilled water to remove salt ions on the surface of the algae, and then use absorbent cotton to dry the surface water. Weigh 200g of copper algae into a 1000mL conical beaker, add 800mL of 80% (V / V) ethanol aqueous solution, seal the mouth of the conical beaker with two layers of safety film, extract in the dark for 48 hours, and filter. Continue to add 800% (V / V) ethanol aqueous solution 800mL again to the copper algae through the above-mentioned leaching treatment, seal the mouth of the triangular beaker with two layers of safety film, leaching in the dark for 48 hours, and filtering. The copper algae was repeatedly extracted in the dark until the extract was colorless;

[0024] Combine the above extracts into a brown bottle, evaporate in vacuum at 20°C, concentrate until the alcohol content of the solutio...

Embodiment 2

[0027] Example 2: Effects of brown algae extracts on the proliferation of bait microalgae Isochrysis (3011) and highly toxic Karo sp.

[0028] Take natural seawater for filtration and disinfection, add KNO separately 3 100mg / L, K 2 HPO 4 10mg / L, MnSO 4 0.25mg / L, FeC 6 h 5 o 7 2.5mg / L, Na 2 EDTA 20mg / L, V B12 0.5mg / L, V B1 5 mg / L of each substance formed a microalgae culture medium. Inoculate dinoflagellates (3011), a commonly used bait for raising shellfish seedlings in aquaculture, into the above-mentioned culture solution. In the bait culture algae liquid, the highly toxic Caro algae (purified from Zhejiang aquaculture waters) were inoculated respectively. The salinity of the culture solution is 20‰, the volume of the culture water is 100mL, and each 6 parallel. Take 3 parallels for each group, respectively add the brown algae extract powder extracted from the above copper algae, fully dissolve, so that the final concentration of the brown algae extract in t...

Embodiment 3

[0030] Example 3: Effects of brown algae extracts on the proliferation of bait microalgae Chlorella and highly toxic Karo sp.

[0031] Take natural seawater for filtration and disinfection, add KNO separately 3 100mg / L, K 2 HPO 4 10mg / L, MnSO 4 0.25mg / L, FeC 6 h 5 o 7 2.5mg / L, Na 2 EDTA 20mg / L, V B12 0.5mg / L, V B1 5 mg / L of each substance formed a microalgae culture medium. Inoculate the common bait microalga Chlorella into the above-mentioned culture solution for raising shellfish seedlings in aquaculture. In the bait culture algae liquid, Caro algae (purified from Zhejiang aquaculture waters) were inoculated respectively. The salinity of the culture solution is 20‰, the volume of the culture water is 100mL, and each 6 parallel. Take 3 parallels for each group, respectively add brown algae extract powder extracted from hijiki according to the method of Example 1, fully dissolve, and make the final concentration of brown algae extract in the algae liquid be 55...

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Abstract

The invention provides a brown algae extract. The extract is added in the expanding propagation culture process of aquaculture bait microalgae and used for inhibiting, preventing and controlling mixotrophism-type toxic dinoflagellate highly-toxic karlodinum which frequently occurs to increase the probability of successful expanding propagation of the bait microalgae, reduce harm to culture organisms, lower risks such as food chain transfer of algal toxin, and achieve health culture. By means of the brown algae extract, the multiplication of the toxic dinoflagellate highly-toxic karlodinum which frequently occurs in the expanding propagation process of the bait microalgae or marine product economical microalgae can be effectively inhibited while normal multiplication of the bait microalgaeis not influenced, the success probability of expanding propagation of the bait microalgae is increased, invasion of harmful dinoflagellate is prevented, the harm to the culture organisms is reduced,and the risk of food chain transfer of the algal toxin is lowered.

Description

technical field [0001] The invention belongs to the technical field of bait microalgae propagation and cultivation, and in particular relates to a brown algae extract and its application in inhibiting the proliferation of toxic and harmful dinoflagellates and highly toxic Karo algae in the process of bait microalgae propagation. Background technique [0002] Feed microalgae is a kind of microalgae mainly isolated, purified and screened from natural waters, and it is a beneficial economical microalgae with high nutrition and high bait effect. The high-biomass expansion and propagation of bait microalgae is an indispensable process for the mass cultivation of a type of microalgae in aquaculture, such as shellfish seedling breeding, animal bait and feed preparation, and water quality regulation of aquaculture water. [0003] However, during the propagation and cultivation of bait microalgae, they are often polluted by toxic and harmful dinoflagellates. Harmful, resulting in br...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/00C12N1/12C12R1/89
CPCA23K50/80A23K10/00C12N1/12Y02A40/81
Inventor 郭贺周成旭王子安张金荣孟冉徐继林严小军
Owner NINGBO UNIV
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