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Bacillus subtilis and application thereof in agricultural production

A technology of Bacillus subtilis and Bacillus, applied in the field of screening and application of functional microorganisms, can solve the problems of unstable use effect, immature research, and few types of products, and achieve the effects of improving quality, realizing agricultural health, and environmental friendliness

Active Publication Date: 2019-08-02
QINGDAO LILIHUI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The research on phosphorus-solubilizing microorganisms at home and abroad has gone through a long time, but due to the wide variety of phosphorus-solubilizing microorganisms, many studies are still not mature enough
At present, the development of phosphorus-solubilizing microbial products in my country is still in the initial stage, and there are relatively few types of commercially available products, the use effect is unstable, and the promotion is difficult.

Method used

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  • Bacillus subtilis and application thereof in agricultural production
  • Bacillus subtilis and application thereof in agricultural production
  • Bacillus subtilis and application thereof in agricultural production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 has the screening of phosphorus-solubilizing functional microorganisms

[0021] 1. Soil sample: Calcareous soil in the mountainous area in the southwest of Qingzhou City, Shandong Province.

[0022] 2. Preparation of soil dilution: Weigh 0.5g soil sample and dissolve it in 4.5ml sterile water to make a 1:10 soil solution, then draw 0.5ml soil solution from it and place it in 4.5ml sterile water to make a 1:100 ratio Soil solution, by analogy with this method, prepare 1:10 6 -10 7 soil dilution solution.

[0023] Take 0.1ml of soil dilution solution and evenly spread it on the insoluble inorganic phosphorus solid medium (glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 ·7H 2 0 0.3g, FeSO 4 .7H 2 0 0.03g, MnSO 4 .4H 2 0 0.03g, Ca 3 (PO 4 ) 2 5.0g, distilled water 1000ml, pH 7.0-7.5, agar 20g, sterilized at 115°C for 30min), cultured upside down in a 30°C incubator for 3 days, observed the colonies growing on the medium, and there were ...

Embodiment 2

[0024] The re-screening of embodiment 2 phosphorus-solubilizing microorganisms

[0025] Inoculate the 12 strains obtained from the preliminary screening in Example 1 on the insoluble inorganic phosphorus solid medium respectively, and after cultivating at 30°C for 3 days, observe the size of the transparent circle around the colony. It was found that the three strains with the largest transparent circle were LLH- -2, LLH--3, LLH--10.

[0026] The three strains with the largest transparent circle were inoculated into 50 mL insoluble inorganic phosphorus liquid medium (glucose 10 g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 .7H 2 0 0.3g, FeSO 4 .7H 2 0 0.03g, MnSO 4 .4H 2 0 0.03g, Ca 3 (PO 4 ) 2 5.0g, distilled water 1000ml, pH 7.0-7.5, sterilized at 115°C for 30min), 30°C, 200rpm, cultivated for 6 days, and at the same time, the liquid phosphate-dissolving medium without any bacteria was used as the control group; The number of live bacteria.

[0027] 2.1 D...

Embodiment 3

[0034] Identification of embodiment 3 LLH-3 bacterial strain

[0035] 1) Colony morphology of LLH-3 strain:

[0036] The colony of the LLH-3 strain is flat, the surface is rough, opaque, the edge is initially smooth and then becomes rough, the colony is white to dirty white, the size of the spore is about 0.5-0.9×0.8-1.3 μm, oval or columnar, mesogenic or Mesophilic; positive Gram reaction, positive catalase reaction, positive V.P reaction, positive starch hydrolysis test, positive indole test, available glucose, arabinose, xylose and mannitol, growth temperature range 20-45 °C, pH range 5-10.

[0037] 2) Molecular biology identification of LLH-3 strain:

[0038] The LLH-3 strain obtained from the above screening was identified by the method of molecular biology, its 16s rDNA sequence SEQ ID NO: 1 was obtained, and blast comparison was performed in the GenBank nucleic acid database.

[0039] SEQ ID NO: 1 is shown below:

[0040]

[0041] Combining the colony morphology ...

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PUM

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Abstract

The invention relates to the technical field of screening and application of functional microorganisms, and specifically provides new bacillus subtilis and application thereof in agricultural production. The bacillus subtilis with the preservation number of CCTCC NO: M2019402 is screened from calcareous soil. The strain of the bacillus subtilis has relatively strong enzyme production ability, thecontent of effective phosphorus in soil can be effectively improved, the crop yield can be increased, common plant diseases such as root rot and scab can also be effectively prevented and controlled,and the effect is remarkable.

Description

technical field [0001] The invention relates to the technical field of screening and application of functional microorganisms, in particular to a novel strain of Bacillus subtilis and its application in agricultural production. Background technique [0002] Phosphorus is an essential nutrient element for plant growth, but the phosphorus element in the soil is easily fixed, resulting in a lack of available phosphorus, so how to convert insoluble phosphorus in the soil into soluble available phosphorus is the key to solving the problem of phosphorus deficiency. Phosphorus-solubilizing microorganisms can activate insoluble phosphorus in soil, which has become one of the current research hotspots. [0003] Phosphate-Solubilizing-Microorganisms (PSM) can convert insoluble phosphate in soil into phosphate that can be absorbed and utilized by plants by using their own metabolites or synergizing with other organisms. There are many types of phosphorus-solubilizing microorganisms in...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05G3/00A01N63/02A01P3/00A01P21/00C12R1/125
CPCC05F11/08C05G3/00C12R2001/125C12N1/205A01N63/10Y02A40/20
Inventor 代庆海孙春龙王凌云王丽宁李灯辉赵梓皓张倩倩刘晓霞
Owner QINGDAO LILIHUI BIOTECH
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