19 results about "Calcareous soils" patented technology

The invention relates to a strain of Chryseobacterium sp. ZJUHULYY1 and application of the Chryseobacterium sp. ZJUHULYY1 in promotion of corn growth and iron absorption, and provides a strain of Chryseobacterium sp. ZJUHULYY1, which is preserved in China Center for Type Culture Collection on April 27, 2025, and has a preservation number of CCTCC NO: M 2025904. The strain of Chryseobacterium sp. ZJUHULYY1 is obtained by separating and purifying from rhizosphere soil of corn, and the strain of Chryseobacterium sp. ZJUHULYY1 is obtained by separating and purifying from rhizosphere soil of corn. The biological characteristics of the strain are as follows: after being cultured in an LB plate culture medium at 30 DEG C for 24 hours, the bacterial colony is round or oval, golden yellow, smooth and glossy in surface, neat in edge and negative in gram staining; when the strain is inoculated to rhizosphere of corn growing in calcareous soil, compared with a control group, the plant height, chlorophyll content, biomass and active iron content of the corn are all remarkably improved, the growth condition of the corn in the calcareous soil is effectively improved, and the absorption and utilization efficiency of the corn on iron elements is improved.

The invention relates to the technical field of microorganisms, in particular to a strain of Senotrophomonas sepilia DB3 with disease-resistant, iron-dissolving and growth-promoting functions and application thereof, and the strain is separated from calcareous soil pear tree rhizosphere and has good capabilities of producing IAA (Indoleacetic Acid), quickly growing, dissolving insoluble iron, producing iron carriers and effectively antagonizing various pathogenic bacteria. Pot experiments show that the DB3 microbial inoculum can significantly promote growth of peanut and pyrus betulaefolia seedlings, improve root development and iron nutrient content, enhance leaf SPAD value and iron absorption and relieve iron-deficiency yellows, can be used for preparing biological bacterial fertilizers and reducing chemical iron fertilizer investment, and has good development and application prospects.

The invention discloses an efficient synergistic calcareous soil phosphorus activation preparation and application thereof. The efficient synergistic calcareous soil phosphorus activation preparation comprises nano TiO2, phosphate solubilizing bacteria and yeast peptide. When the fertilizer is applied to calcareous soil crop planting, the phosphorus absorption of plants is improved. Through combined application of nano TiO2, phosphate solubilizing bacteria and yeast peptide, the pH value of calcareous soil can be reduced, fixed-state inorganic phosphorus in the soil is activated, the content of available phosphorus in the soil is increased, and supply of inorganic phosphorus in a soil-plant system is promoted. Through combined application of nano TiO2, phosphate solubilizing bacteria and yeast peptide, plants can obtain phosphorus nutrients in calcareous soil, and growth of crops is promoted.

The present invention relates to the technical field of safe utilization of contaminated cultivated land, and particularly relates to a safe utilization method applicable to slightly and moderately cadmium-contaminated calcareous soil. The present invention provides a safe utilization method applicable to slightly and moderately cadmium-contaminated calcareous soil. Wheat is planted on the cadmium-contaminated calcareous soil, and the grains of the harvested wheat are used as seeds after maturity; in the next wheat growing season, the seeds are planted in uncontaminated clean soil, and wheat grains meeting the national food safety standards are harvested after maturity. This method can not only ensure the safe utilization of cadmium-contaminated cultivated land, no secondary pollution of clean soil, and the green and safe production of wheat, but also has low cost, convenient operation, and is easy to promote, achieving a win-win situation of economic, ecological, and social benefits.

The present application belongs to the technical field of biochar-based fertilizer, and particularly relates to a preparation method and application of a biochar-based zinc sulfate fertilizer for relieving zinc deficiency stress of calcareous soil. The biochar-based zinc sulfate fertilizer comprises a mother granule and a first coating, a second coating and a third coating successively coated on the surface of the mother granule; the mother granule is prepared by mixing and granulating urea, diammonium phosphate and potassium sulfate; the first coating comprises sulfur and bentonite; the second coating comprises zinc oxide powder, ammonium bicarbonate and ammonia water; and the third coating comprises biochar. The biochar-based zinc sulfate fertilizer can quickly and efficiently increase the effective zinc content of calcareous soil in the Loess Plateau, so as to cope with the zinc deficiency stress of the soil and promote crop growth.

The application belongs to the technical field of bioengineering, and provides an encoding gene for enhancing the tolerance of plants to iron deficiency stress and application thereof. The application solves the problem that soybeans are restricted in growth in an iron deficiency environment, especially in calcareous soil and soda saline-alkali soil, by mining and applying the encoding gene (nucleotide sequence as shown in SEQ ID No. 1) of a soybean GmMYB14 transcription factor. Experiments prove that the gene can significantly enhance the tolerance of plants to iron deficiency stress after overexpression in Arabidopsis thaliana and soybeans. The application provides a key gene resource for molecular breeding: GmMYB14 effectively promotes the absorption and utilization of iron in plants by regulating the phenylpropanoid metabolic pathway, which not only can improve the planting adaptability of soybeans in low-iron soil, but also can improve the yield and quality of soybeans, thereby providing strong technical support for expanding the planting area of soybeans in iron deficiency soil areas, and having important significance for relieving the insufficient production capacity of soybeans in China and improving the self-sufficiency rate.

Carrier-based agricultural biofertilizer composition, comprising an isolated Bacillus megaterium strain CGAPGPBBS-034 of deposit accession number KY495205; and an agriculturally-acceptable liquid formulation with a stabilizer. The strain may be isolated from alkaline calcareous soil of rhizosphere and rhizoplane from Yola, Nigeria; e.g., by serial dilution and spread plate method. Also disclosed method for enhancing soil using a biofertilizer composition, comprising: preparing a nutrient medium containing carbon (e.g., sucrose, glucose, molasses), nitrogen (e.g., ammonium sulphate, urea, peptone), and phosphate sources (e.g., calcium / potassium / sodium phosphate); inoculating the medium with pure culture of strain CGAPGPBBS-034, allowing it to undergo fermentation; harvesting the bacterial cells; fermenting the broth at 3.0×10^9 CFU / mL and zero total dissolved solids water, incorporating polyvinyl pyrrolidone (PVP) at 0.5%; diluting the formulation to microbial concentration of 3.0×10^8 CFU / mL; and applying formulation to soil (e.g., sprinkler, spray, seed deepening). The bacterial formulation may be mixed with a carrier to provide stability in soil.

Carrier-based agricultural biofertilizer composition, comprising an isolated Bacillus megaterium strain CGAPGPBBS-034 of deposit accession number KY495205; and an agriculturally-acceptable liquid formulation with a stabilizer. The strain may be isolated from alkaline calcareous soil of rhizosphere and rhizoplane from Yola, Nigeria; e.g., by serial dilution and spread plate method. Also disclosed method for enhancing soil using a biofertilizer composition, comprising: preparing a nutrient medium containing carbon (e.g., sucrose, glucose, molasses), nitrogen (e.g., ammonium sulphate, urea, peptone), and phosphate sources (e.g., calcium / potassium / sodium phosphate); inoculating the medium with pure culture of strain CGAPGPBBS-034, allowing it to undergo fermentation; harvesting the bacterial cells; fermenting the broth at 3.0×10^9 CFU / mL and zero total dissolved solids water, incorporating polyvinyl pyrrolidone (PVP) at 0.5%; diluting the formulation to microbial concentration of 3.0×10^8 CFU / mL; and applying formulation to soil (e.g., sprinkler, spray, seed deepening). The bacterial formulation may be mixed with a carrier to provide stability in soil.

The application belongs to the technical field of microorganisms and particularly relates to a strain of DB3 with disease-resistant iron-dissolving and growth-promoting functions Stenotrophomonas sepilia The strain DB3 is isolated from the rhizosphere of pear trees in calcareous soil, has good abilities of IAA production, rapid growth, dissolution of insoluble iron, iron carrier production and antagonism to various pathogenic bacteria. Through potting tests, it is shown that the DB3 bacterial agent can significantly promote the growth of peanut and pear seedlings, improve the root development and iron nutrient content, enhance the SPAD value and iron absorption of leaves, and relieve the iron deficiency chlorosis, and can be used for preparing a biological bacterial fertilizer, reducing the input of chemical iron fertilizer, and has a good development and application prospect.

The invention relates to the technical field of fertilizers, and discloses a selenium-rich slow-release fertilizer for grapes in a sierozem-sandy loam area and a preparation method thereof.The selenium-rich slow-release fertilizer for the grapes comprises a selenium source, a fertilizer base, a fertilizer base, a fertilizer base, a fertilizer base, a fertilizer base, a fertilizer base and a fertilizer base, the chelating agent is prepared from humic acid-Zn and EDTA (Ethylene Diamine Tetraacetic Acid)-Ca; the selenium activating agent is montmorillonite modified by ammonium dihydrogen phosphate; an anti-fixation agent, wherein the anti-fixation agent is a complex of L-proline and selenium; the slow-release carrier is a coating film formed by polycaprolactone or polylactic acid and humic acid; the quick-acting carrier is pH response type gel formed by cross-linking gamma-polyglutamic acid and Ca < 2 + >; micromolecular soybean protein titanium; the invention relates to a lignin-acrylic acid grafted copolymer. Through slow / quick-acting double carriers and a multiple chelation anti-immobilization system, the beneficial effects of high bioavailability and staged on-demand release of selenium in sierozem are obtained.

The invention discloses a method for promoting growth of intercropping crops based on a soil micro food web and application of the method, and belongs to the technical field of crop planting. According to the invention, it is found for the first time that by adding a specific type of bacterial-feeding nematodes into soil, synergistic improvement of growth indexes such as plant height, stem diameter and biomass of two crops can be obviously and stably promoted; the application method is simple and easy to operate, the cost is controllable, and no additional farming equipment or steps are needed; the method is high in environmental compatibility, is particularly suitable for a farmland peanut-corn intercropping system in alkaline and calcareous soil, can effectively improve the crop growth performance, farmland productivity and agricultural product yield and quality, can optimize the soil ecological function, continuously increases the economic benefits of farmers, and has wide application prospects. A brand-new, low-cost and easy-to-popularize candidate scheme is provided for agricultural green sustainable development of the alkaline soil area in North China, and the application prospect is wide.

The application discloses a kind of high-efficiency synergic calcareous soil phosphorus activation preparation and application thereof, comprising: nano TiO2, phosphorus-solubilizing bacteria and yeast peptide.It is applied to calcareous soil crop planting, and the absorption of plant to phosphorus is improved.Nano TiO2 is applied with phosphorus-solubilizing bacteria and yeast peptide, and the pH of calcareous soil can be reduced, the fixed state inorganic phosphorus in soil is activated, the content of effective phosphorus in soil is improved, and the supply of inorganic phosphorus in soil-plant system is promoted.Nano TiO2 is applied with phosphorus-solubilizing bacteria and yeast peptide, and it can help plant to obtain phosphorus nutrient in calcareous soil, and promote the growth of crop.

The application discloses a special nail fertilizer for peach trees suitable for lime soil in the north. The special nail fertilizer for peach trees comprises a high-nitrogen tip formula, a high-phosphorus and high-potassium middle formula and a high-potassium end formula; the mass percentage of nitrogen, diaphosphorus pentoxide and potassium oxide in the high-nitrogen tip formula is 34:4:5, the mass percentage of nitrogen, diaphosphorus pentoxide and potassium oxide in the high-phosphorus and high-potassium middle formula is 9:11:24, and the mass percentage of nitrogen, diaphosphorus pentoxide and potassium oxide in the high-potassium end formula is 6:2:39; the potassium humate content of the nail fertilizer is greater than or equal to 8%. The nail fertilizer has great advantages in yield and quality, and the single fruit weight, average yield, average soluble sugar content, average soluble solid, average reduced vitamin C are all significantly improved, and the titratable acid content and nitrate content are both decreased; moreover, the soil pH treated by the nail fertilizer shows a downward trend, indicating that the nail fertilizer has a conditioning and improvement effect on the lime soil pH, and is more beneficial to the growth of the peach trees.

This invention discloses a synergistic water-soluble fertilizer composition of macroelements and its application, relating to the field of agricultural fertilizer technology. The composition includes the following components: macroelements comprising nitrogen, phosphorus pentoxide, and potassium oxide, with a mass ratio of 20:20:20; ammonium polyphosphate, potassium dihydrogen phosphate, sulfur-based raw materials, and chelated trace elements; L-type acidic amino acids; and soluble organic matter (DOM). This synergistic water-soluble fertilizer composition of macroelements and its application, by compounding ammonium polyphosphate and potassium dihydrogen phosphate at a mass ratio of 1:(0.1-10) and using L-type acidic amino acids as chelating carriers, allows the chelating ability of ammonium polyphosphate for calcium and magnesium ions and the transport capacity of L-type acidic amino acids for trace elements to synergistically superimpose, thereby effectively inhibiting the formation of insoluble phosphate precipitates by the combination of phosphorus with calcium and magnesium ions in hard water or calcareous soil environments.

The invention provides a calcareous soil exchangeable salt base and salt base total amount determination method, which comprises the following steps: putting soil to be measured and a magnetic stirrer into a centrifuge tube, adding an ammonium chloride-ethanol exchange liquid, putting the centrifuge tube on a magnetic stirrer, carrying out magnetic stirring for 3-5 minutes while keeping vortex on the liquid surface in the stirring process, and filtering to obtain filtrate; and filtering and collecting filtrate, and determining and analyzing the exchangeable salt base and the total amount of the salt base in the calcareous soil. According to the method, the overnight standing time is saved, and more than 110mL of 70% ethanol solution for salt leaching can be saved when one sample is measured; the exchange reaction only needs one time, and the reaction time is only 3 minutes; the pH value of the ammonium chloride-ethanol exchange liquid does not need to be adjusted; according to the method, 50mL of ammonium chloride-ethanol exchange liquid is needed for each sample, and compared with a corresponding method for measuring each sample, the usage amount of ammonium chloride is saved by 80%, and the usage amount of 70% ethanol is saved by 80%. The working efficiency is greatly improved; and the reagent usage amount is reduced.

The invention belongs to the technical field of extraction, and discloses a combined extraction method of quick-acting phosphorus suitable for acidic, neutral and calcareous soil, which comprises the following steps: preparing a composite extraction agent which comprises the following components: citric acid-sodium citrate buffer solution, EDTA (Ethylene Diamine Tetraacetic Acid) disodium salt and ammonium oxalate; mixing the soil sample with the composite extracting agent; carrying out solid-liquid separation on a mixture of the soil sample and the composite extracting agent; and carrying out phosphorus determination on the filtrate obtained by solid-liquid separation. The citric acid-sodium citrate buffer solution is used for maintaining an acidic environment and dissolving iron / aluminum combined phosphorus; eDTA disodium salt is chelated with calcium ions, so that refixation of phosphorus in calcareous soil is prevented; according to the method, the leaching process is simple, the efficiency is high, the universality is high, the recovery rate is high, the method is suitable for the soil with the pH value of 4.0-8.5, the recovery rate is larger than or equal to 92%, the leaching cost is low, the method is environmentally friendly, the composite leaching agent is free of toxicity and harm and fluoride and strong acid components, waste is easy to treat, and the method meets the green chemical standard.

This invention discloses a water-soluble fertilizer package specifically for peach trees in calcareous soils of northern China and its application method. The package includes a bud-germinating fertilizer, a core-hardening fertilizer, a fruit-enlarging fertilizer, and a post-harvest fertilizer. In the bud-germinating fertilizer, the nitrogen content is 36%, the phosphorus content (P) is 5% (based on the mass of P2O5), and the potassium content (K) is 6% (based on the mass of K2O). In the core-hardening fertilizer, the nitrogen content is 11%, the P content (based on the mass of P2O5) is 12%, and the potassium content (based on the mass of K2O) is 27%. In the fruit-enlarging fertilizer, the nitrogen content is 7%, the P content (based on the mass of P2O5) is 4%, and the potassium content (based on the mass of K2O) is 39%. In the post-harvest fertilizer, the nitrogen content is 21%, the P content (based on the mass of P2O5) is 10%, and the potassium content (based on the mass of K2O) is 19%. Application tests of the water-soluble fertilizer of this invention show that, on average, the amount of fertilizer used is reduced by more than 25%, the yield is higher than that of conventional fertilization treatment, and the quality is improved; the soil pH also shows a downward trend, indicating that the fertilizer of this invention has a certain effect on regulating soil acidity and alkalinity.

The invention discloses a preparation method of a CMAKG-SA slow-release water-retaining agent, which comprises the following steps: firstly, carrying out carboxymethylation treatment on Sa-son seed gum to obtain carboxymethylated Sa-son seed gum CMAKG, then taking the carboxymethylated Sa-son seed gum as a matrix, taking acrylic acid of which the neutralization degree is adjusted by a potassium hydroxide solution as a reaction monomer, taking N, N-dimethylformamide as a solvent, and carrying out a reaction for 2-3 hours to obtain the CMAKG-SA slow-release water-retaining agent. The preparation method comprises the following steps: taking carboxymethyl artemisia seed gum as a raw material, taking N, N '-methylene bisacrylamide as a cross-linking agent, adding urea as a slow-release nitrogen fertilizer, stirring and mixing, fully swelling the carboxymethyl artemisia seed gum, dropwise adding ammonium persulfate into the mixed solution, generating sodium alginate SA hydrogel through a polymerization reaction, and cross-linking the sodium alginate SA hydrogel with CMAKG to finally prepare the carboxymethyl artemisia seed gum-based composite super absorbent resin. The invention discloses a CMAKG-SA slow-release water-retaining agent. The SA is added into the CMAKG in a cross-linking manner, so that the water retention capacity of aeolian sandy soil, sierozem and gravelly soil can be remarkably improved, the water absorption capacity of the CMAKG-SA reaches 497.32 g / g, after the CMAKG-SA is used, the water potential decreasing trend of leaves is slowed down, and the yield loss under drought stress is reduced.

The present application relates to the technical field of safe utilization of farmland soil with heavy metal composite pollution, and particularly relates to a wheat conditioner suitable for lime soil with composite pollution. The conditioner is used for spraying on the leaves of wheat and is composed of EDTA-Zn and fulvic acid as effective components and a surfactant and water. Through the antagonistic effect between Zn element in EDTA-Zn and Cd element and the synergistic effect between EDTA-Zn and fulvic acid, the conditioner can reduce the transfer and enrichment of heavy metals to wheat grains when wheat is planted in lime soil with moderate and light heavy metal Cd and As composite pollution. Moreover, the conditioner has simple components and low cost, and will not cause secondary pollution to soil and underground water.