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A kind of apolipoprotein e detection kit

A technology for detecting kits and apolipoproteins, which is applied in the field of medical testing and can solve problems such as deviations in biochemical results

Active Publication Date: 2020-11-03
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some biochemical results may be biased due to the large size of chylomicron particles that scatter incident light

Method used

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  • A kind of apolipoprotein e detection kit
  • A kind of apolipoprotein e detection kit
  • A kind of apolipoprotein e detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 apolipoprotein E detection kit

[0025] The component of reagent 1 is R1:

[0026]

[0027] R2: detection reagent 2

[0028] Phosphate buffer 100mmol / L

[0029] Goat anti-human ApoE antibody 14.2ml / L

Embodiment 2

[0030] The comparative experiment of embodiment 2 degreasing agent

[0031] A proper amount of perfluoropolyether liquid is added to the hyperlipidemic serum sample, which is divided into upper and lower layers after being treated with perfluoropolyether. The upper layer is the separated blood lipid component, and the lower layer is the clarified serum part. First measure the ApoE item, then add 0.1mL reagent according to the reagent instructions, oscillate and mix evenly, let stand for 5min, and finally centrifuge at 6000rpm for 5min, use a sampling gun to absorb about 0.3mL of the clear liquid under the surface coagulum, and measure the above ApoE item again. The detection data were corrected according to the dilution factor. SPSS17.0 software was used for statistical processing. The average deviation (Bias%) of ApoE indicators before and after treatment was <1 / 2 the US Clinical Medical Laboratory Amendment Regulations (CLIA'88) allowed the total error (TEa) to be a clinical...

experiment example 1

[0032] Experimental example 1 control group original sample testing result and adding artificial fat emulsion, the result of ApoE index after lipid scavenger treatment is shown in Table 1, the result shows that before and after the ApoE item is processed, the average deviation (Bias%) of this item<1 / 2TEa , is a clinically acceptable level, and its error is caused by random error.

[0033]

[0034] Table 1 Test results of 40 cases of control group specimens before and after treatment

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Abstract

The invention relates to a reagent for removing lipids in hyperlipidemia serum and an application of the reagent to an apolipoprotein E detection process. A method comprises the steps of mixing the serum with the reagent for removing the blood lipids; and stirring and uniformly mixing the serum and the reagent, and carrying out standing for 10 minutes, so that the serum is layered, wherein the lower layer is blood-lipid-free serum, and the upper layer is a white separated blood lipid part.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a reagent for treating hyperlipidemia and its application in detection of serum apolipoprotein E. Background technique [0002] Lipidemia is a common interference phenomenon in the detection of clinical biochemical items. Its plasma is turbid. If there are too many chylomicrons, the plasma can be milky white, so it is also called "chylus blood". Some biochemical results may be biased due to the large size of chylomicron particles that scatter incident light. In recent years, many researchers have used methods such as vacuum ultracentrifugation, high-speed centrifugation, PEG precipitation or ether treatment to eliminate the interference of lipemia on routine biochemical projects, but each has its advantages and disadvantages. LipoClear is not suitable for glucose, sodium, potassium, chloride, phosphate, magnesium, CK-MB, ALP, GGT, total protein, albumin, CRP, and troponin T meas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/31G01N1/34G01N1/40
CPCG01N1/34G01N1/4044G01N21/31G01N21/78
Inventor 华权高吴年芬伍卫姣梁艳黄爱龚婷沈鹤霄赵畅徐春雷
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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