Method for synthesizing nortestosterone artificial antigen
A technology of nortestosterone and artificial antigen is applied in the field of synthesis of nortestosterone artificial antigen, which can solve the problems of low binding rate, influence on immune effect and the like, and achieve the effects of improving lambing rate, good immune effect and high yield.
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[0033] A kind of synthesis method of nortestosterone artificial antigen
[0034] Through the methods of reproductive immunology and reproductive endocrinology, the technology of artificially controlling ewes to give birth to twin lambs is a new biological reproduction technology with low cost, good effect, simple operation and no side effects. Rate and lambing rate of steroidal antigens. The invention uses a novel composite catalyst to successfully couple nortestosterone and BSA to prepare nortestosterone artificial antigens.
[0035] 1. Experimental materials:
[0036] 19-nortestosterone (NT): purchased from Shanghai Guandao Bioengineering Co., Ltd.;
[0037] Bovine serum albumin (BSA): (content 96%) Yancheng Saibao Biotechnology Co., Ltd.;
[0038] The rest of the reagents were of analytical grade.
[0039] Dialysis bags (8000-14000Da) Solarbio products;
[0040] Phosphate buffered saline (PBS, pH 7.4);
[0041] Attapulgite: Haodi Industry and Trade Co., Ltd., Jingyuan...
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