Method for synthesizing nortestosterone artificial antigen

Abstract

The invention discloses a method for synthesizing nortestosterone artificial antigen. The method comprises the following steps: (1) preparing the attapulgite rod-nano iron oxide powder by purifying the attapulgite, activating the attapulgite and mixing with the nano iron oxide powder; (2) after 19-nortestosterone hapten is stirred and dissolved with N,N-dimethylformamide, adding the attapulgite rod-nano iron oxide powder obtained in the step (1) to obtain a solution 1; (3) dissolving bovine serum albumin in PBS, adjusting a pH value, adding N, N-dimethylformamide, and stirring the materials todissolve the solution 2; and (4) adding 1 drop of the solution 1 obtained in the step (2) to the solution 2 obtained in the step (3), and performing a light-shielding reaction, after the reaction iscompleted, performing dialysis by using distilled water, and then performing dialysis by using PBS, performing packaging when a UV-scanning dialysate has no small molecule absorption peaks, and refrigerating the material to obtain the product. The method prepares the nortestosterone artificial antigen by nortestosterone and BSA through the attapulgite rod-nano iron oxide powder.

Description

technical field [0001] The invention belongs to the field of veterinary biotechnology, and in particular relates to a method for synthesizing an artificial antigen of nortestosterone. Background technique [0002] The amide bond is one of the most basic chemical structures found in nature, which constitutes the backbone of important biological peptides and proteins, and exists in many natural products and compounds such as drugs. The compounds developed by the three major international pharmaceutical companies were sampled and counted, and 55% of the compounds had amide bonds. The reduction of amides to prepare amines is also a common transformation in organic synthesis. At present, the main method for forming amide bonds is still the coupling reagent method with poor atom economy. Catalyzed direct amidation reaction means that without adding any activating reagent, carboxylic acid and amine are condensed under the action of catalyst to form amides. This method can avoid ...

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Problems solved by technology

[0004] Some scientific research institutes at home and abroad have carried out research on steroidal antigens, prepared various types of antigens, and all used chemically synthesized antigens. At present, the main problem is that the molecular binding ratio of BSA and nortestosterone is mostly about 1:20, because the combination The rate is low, affecting the immune effect

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