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Method for regulating and controlling electrochemiluminescence signal by surface charge density of tapered micron-sized hole

A technology of surface charge density and electrochemistry, applied in the field of analytical chemistry, achieves the effects of convenient operation, broad application prospects, and simple experimental equipment

Inactive Publication Date: 2019-08-16
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently there are few reports on sensors that regulate the magnitude of the current through the charge density of micro-nanopores and then regulate the electrochemiluminescent signal of the system.

Method used

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  • Method for regulating and controlling electrochemiluminescence signal by surface charge density of tapered micron-sized hole
  • Method for regulating and controlling electrochemiluminescence signal by surface charge density of tapered micron-sized hole

Examples

Experimental program
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Effect test

Embodiment 1

[0014] (1) The tapered microtube was obtained by drawing the glass capillary, and the tip of the tapered microtube was polished to 3.2 µm with a needle breaker, and then immersed in 10% 3-aminopropyltriethoxysilane (ethanol as the solvent) solution, reacted in vacuum at 60°C for 1.5h, rinsed with ethanol, and dried in a vacuum oven to obtain amino-functionalized microtubes; In a dianhydride (dimethyl sulfoxide as solvent) solution, react in vacuum at 60°C for 1.5 h, rinse with ethanol, and dry in a vacuum oven to obtain carboxyl-functionalized microtubes, which are carboxyl / microtubes;

[0015] (2) Immerse the carboxyl-functionalized microtube in 10 µL, pH=7.0 PBS buffer (2.5 µM OTA aptamer, 1 mg / mL EDC, 2 mg / mL NHS), and react at 37 °C for 12 h to obtain OTA Aptamer functionalized microtubes, that is, aptamer / carboxy / microtubes;

[0016] (3) Immerse aptamer-functionalized microtubes in 10 µL Tris-HCl buffer containing 2 ng / mL OTA, and react at 37 °C for 2 h to obtain OTA-fun...

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PUM

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Abstract

The invention discloses a method for regulating and controlling electrochemiluminescence detection by surface charge density of a tapered micron-sized hole. According to the method, the micron-sized hole is used as an analysis element; an Ru(phen)32+ / TPrA research model is used for researching that a system current is regulated and controlled by the surface charge density of the tapered micron-sized hole to regulate and control the faraday current transmission efficiency of an Ru(phen)32+ / TPrA oxidation reaction; and different electrochemiluminescence signals are generated. The regulation andcontrol of the electrochemiluminescence signals by the surface charge density of the tapered micro-sized hole are realized for the first time; and an experiment device is simple in structure, low incost and convenient to use, widens the application range of electrochemiluminescence, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for regulating the electrochemiluminescent signal by the charge density on the surface of a tapered micropore. Background technique [0002] Electrochemiluminescence is to apply a certain voltage or pass a certain current to generate a luminescent signal in a system containing a luminescent indicator, and to achieve qualitative and quantitative analysis of the target by measuring the luminescence spectrum and intensity. At present, the electrochemiluminescence sensor mainly realizes the quantitative analysis of the target by regulating the content of the luminescent indicator on the working electrode, the luminous efficiency of the luminescent indicator, and the transfer efficiency of electrons on the electrode interface. Micro-nano channel analysis technology uses micro-nano channel as the analysis element. Under the drive of external voltage, the surface...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/327G01N27/30
CPCG01N21/763G01N27/305G01N27/308G01N27/3275
Inventor 林振宇黄艳玲郭隆华邱彬
Owner FUZHOU UNIV
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