Cell or particle counting method based on surface acoustic wave technology

Abstract

The invention provides a cell or particle counting method based on the surface acoustic wave technology. The existing cell counting methods are either only suitable for bulky cell counting or expensive, or take too long times. According to the cell or particle counting method provided by the invention, a microfluidic channel is manufactured on a surface acoustic wave propagation path of a surfaceacoustic wave device; a micro pump is connected with an injector to convey cell fluid or particle fluid into the microfluidic channel, the cell fluid or particle fluid is driven by the sheath flow technology to achieve focusing, so that the cells or particles in the microfluidic channel present single arrangement, and the cells or particles maintain a distance therebetween and sequentially flow bythe microfluidic channel; and the surface acoustic wave device is connected with a detecting instrument, after the detecting instrument works, transmission parameters are measured, and time-varying images of values of the transmission parameters at resonant frequency points are drawn to obtain cell or particle number information. The cell or particle counting method provided by the invention is realized by testing the transmission parameters, data are tested in real time, and the technical precision is improved.

Description

technical field [0001] The invention relates to cell counting technology, in particular to a cell or particle counting method based on surface acoustic wave technology. Background technique [0002] Cells are the most basic units that constitute the structure and function of organisms, and perform various functions of living organisms. Every day in the human body, many cells (RBC) age and die and many cells are regenerated to maintain the dynamic balance of the metabolism of the living body. But if this dynamic balance is disrupted, it will cause a series of health problems. By detecting the number of specific cells, certain diseases can be identified and diagnosed, so cell counting is of great significance to health care, disease treatment, and medical research, and it is also a very useful tool. For example, the CD+4 lymphocytes in the human body infected with HIV will gradually decrease, and the detection of the number of CD+4 lymphocytes can assist in the diagnosis of ...

AI Technical Summary

Problems solved by technology

Although this method has fast detection speed and high detection accuracy, the supporting equipment is expensive, the operation is complicated, the detection cost is high, and the operating environment requirements are high. It is difficult to popularize in developing countries or remote areas, and its function is not only for cells. count a case

This will be somewhat wasteful for detection experiments that only have specific requirements

[0006] Image cytometer technology: a new type of cell detection technology combined with microscope and flow cytometry, combining optical precision instrument measurement of cell morphological parameters with image processing technology, not only can measure a large number of cells, but also can study the morphology of specific cells, and obtain cell follow-up Time-varying information and morphological characteristics of cells inside the tissue, but this imaging method takes a long time and cannot obtain the desired results in real time

[0007] In view of the above-mentioned disclosed cell counting method, there are several defects. Although red blood cell counting can quickly obtain the result, the result may be high, and it is only suitable for large-volume cell counting; flow cytometry has fast detection speed and high accuracy. However, the price is expensive, and it is difficult to popularize in remote areas; the imaging time of image cytometer is too long, and the results cannot be obtained in real time

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