A method for simultaneously measuring the contents of nicotine, 3-(pyrrolidin-2-yl)pyridine, pyridylpyrrolidone and testosterone in serum

Abstract

The invention discloses a method for simultaneously measuring the content of nicotine, 3-(pyrrolidine-2-yl) pyridine, (-)-cotinine and testosterone in serum. The method has the advantages of high simplicity, great rapidity, high detection sensitivity, strong pertinency and wide application range. It is verified that the method has a relatively great recycling property, good linear relation and relatively low quantitation limit for the measurement of the four target objects, can simultaneously detect a plurality of kinds of target objects at one time, and is rapid and efficient. The pretreatment method, liquid phase method and some liquid-mass methods of the method of the invention are also applicable to the analysis of other object objects similar to the above four components in serum, andtherefore, the method of the invention has high applicability.

Description

technical field

[0001] The invention belongs to the technical field of detection, and specifically refers to a method for simultaneously measuring the contents of nicotine, 3-(pyrrolidin-2-yl)pyridine, pyridylpyrrolidone and testosterone based on ultra-high performance liquid phase tandem mass spectrometry. Background technique

[0002] Nicotine, commonly known as nicotine, is an important component of tobacco, which can cause addiction or dependence. Repeated use of nicotine also increases heart rate and blood pressure and reduces appetite. It is also the primary factor in tobacco product addiction. Nicotine can be metabolized into various substances in the human body, among which 3-(pyrrolidin-2-yl)pyridine and pyridine pyrrolidone are one of its main metabolites. The concentration level of nicotine and its metabolites in the blood is closely related to the exposure level of the human body in smoke, so its concentration is used to evaluate smoking behavior and smoke exposu...

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