Application of Platycodon grandiflorum total polysaccharide in the preparation of medicine for the treatment of apoptosis induced by cccp

A technology of total polysaccharides and Platycodon grandiflora, which is applied in the application field of medicine, can solve the problems such as apoptosis of total polysaccharides of Platycodon grandiflorum that have not yet been seen, and achieve the effects of alleviating cell nucleus damage, expanding medical applications, and inhibiting potential changes

Active Publication Date: 2022-07-01
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the anti-apoptosis induced by CCCP.

Method used

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  • Application of Platycodon grandiflorum total polysaccharide in the preparation of medicine for the treatment of apoptosis induced by cccp
  • Application of Platycodon grandiflorum total polysaccharide in the preparation of medicine for the treatment of apoptosis induced by cccp
  • Application of Platycodon grandiflorum total polysaccharide in the preparation of medicine for the treatment of apoptosis induced by cccp

Examples

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Embodiment 1

[0038] Example 1: Preparation and structural analysis of total polysaccharides from Platycodon grandiflorum

[0039] 1. Preparation of Platycodon grandiflorum total polysaccharide:

[0040] The platycodon root was sliced, soaked in 8 times by weight of water overnight, then leached at 80°C for 4 hours, filtered, and the filtrate was heated and concentrated to a relative density of 1.05-1.15 (measured at 50°C), and removed by centrifugation at 3000rpm for 10min after cooling. Impurities, get the leaching solution;

[0041] Slowly add 95% ethanol by volume to the extract, stir while adding to make the final concentration of ethanol 80%, let stand for 24 hours, centrifuge, separate the precipitate, and vacuum dry the precipitate at 60°C to prepare Platycodon grandiflorum total polysaccharides (PGPS) t ).

[0042] 2. Structural analysis of total polysaccharides of Platycodon grandiflorum:

[0043] Infrared spectrum analysis, molecular weight analysis, monosaccharide compositio...

Embodiment 2

[0070] Example 2: Study on the protective effect of total polysaccharide of Platycodon grandiflorum on CCCP-induced apoptosis of porcine alveolar macrophages

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Abstract

The invention discloses the application of the total polysaccharide of Platycodon grandiflorum in preparing a medicine for treating cell apoptosis induced by CCCP. The invention uses CCCP to establish a cell apoptosis model caused by mitochondrial pathway, and studies the effect of Platycodon grandiflorum total polysaccharide on cell activity and cell apoptosis after CCCP induces oxidative damage in porcine alveolar macrophages. The present invention finds for the first time that a certain concentration of Platycodon grandiflorum total polysaccharide (100-200 μg / mL) can antagonize cell apoptosis caused by CCCP, significantly reduce the phenomenon of nuclear deformation, and inhibit the change of mitochondrial membrane potential. The invention expands the medical application of the total polysaccharide of Platycodon grandiflorum, and is beneficial to the development of medicines with new indications that use the total polysaccharide of Platycodon grandiflorum as an effective ingredient.

Description

technical field [0001] The invention relates to the field of medical application of total polysaccharide of Platycodon grandiflorum, in particular to the application of total polysaccharide of Platycodon grandiflorum in preparing a medicine for treating apoptosis induced by CCCP. Background technique [0002] Carbonyl cyanide m-chlorophenylhydrazone (CCCP) is an uncoupling agent, which has the function of inhibiting mitochondrial oxidative phosphorylation, which can increase the consumption of intracellular oxygen, make cells in a hypoxic state, and destroy the biological oxidation and phosphorylation phase. coupling effect. When the electron transport chain process is normal, CCCP inhibits the phosphorylation process of converting ADP to ATP by eliminating the mitochondrial transmembrane proton concentration gradient, resulting in the impact of protein synthesis in mitochondria, resulting in cell damage step by step until cell apoptosis Death. [0003] Platycodon grandifl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61K36/34A61P35/00C08B37/00
CPCA61K31/715A61K36/34A61P35/00C08B37/0003C08B37/006A61K2236/331A61K2236/333A61K2236/39
Inventor 赵晓娜刘建柱王成程国栋
Owner SHANDONG AGRICULTURAL UNIVERSITY
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