50results about How to "Expansion of medical use" patented technology

The invention relates to an anti-CD20 monoclonal antibody, a preparation method and an application of the anti-CD20 monoclonal antibody; Chinese hamster ovary cells CHODG44 are adopted to prepare the anti-CD20 monoclonal antibody consistent to rituximab, thereby establishing a large-scale high-expression production technology for 300L of eukaryocytes, wherein the protein expression quantity is more than 1.2g / L and the protein purification yield is improved to more than 60%. The anti-CD20 monoclonal antibody is safe, effective, relatively strong in practicability, low in cost, convenient and quick, and the preparation technology of the anti-CD20 monoclonal antibody is simple and convenient, easy to operate and use and obvious in curative effect, thereby providing a new source of medicines for the B cell induced malignant tumors, particularly the non-hodgkin lymphoma, autoimmune disease, renal failure, hepatitis B, hepatitis C and diabetes, etc.; the anti-CD20 monoclonal antibody is applied to the large-scale production and commercial application in the industries such as medicine, biotechnology and the like and has a good application prospect, remarkable social benefit and economic benefit.

The invention relates to a traditional Chinese medicine extract with adenosine deaminase inhibition activity and an application of the traditional Chinese medicine extract. A preparation method of thetraditional Chinese medicine extract comprises the steps of taking traditional Chinese medicinal material powder, adding methanol, performing ultrasound extraction under a sealed condition, after theultrasound extraction, complementing the lost weight with methanol, performing centrifugation, taking a supernate, performing nitrogen blow-drying to form an extract, namely the traditional Chinese medicine extract, wherein traditional Chinese medicines are radix bupleuri, ligusticum wallichii, Chinese angelica, radix sophorae flavescentis, artemisia capillaris, bighead atractylodes rhizome, folium isatidis, a glossy privet fruit, schisandra chinensis, gardenia, rheum palmatum, sinensis, rhizoma cimicifugae, pollen pini, polygonum cuspidatum or sedum sarmentosum. After determination by established liquid chromatography-tandem mass spectrometry, the traditional Chinese medicine extract has a stronger inhibiting effect on adenosine deaminase, and cell experiments and animal experiments further confirm that the traditional Chinese medicine extract has a significant treatment effect on leukemia and the like.

The invention discloses the application of the total polysaccharide of Platycodon grandiflorum in preparing a medicine for treating cell apoptosis induced by CCCP. The invention uses CCCP to establish a cell apoptosis model caused by mitochondrial pathway, and studies the effect of Platycodon grandiflorum total polysaccharide on cell activity and cell apoptosis after CCCP induces oxidative damage in porcine alveolar macrophages. The present invention finds for the first time that a certain concentration of Platycodon grandiflorum total polysaccharide (100-200 μg / mL) can antagonize cell apoptosis caused by CCCP, significantly reduce the phenomenon of nuclear deformation, and inhibit the change of mitochondrial membrane potential. The invention expands the medical application of the total polysaccharide of Platycodon grandiflorum, and is beneficial to the development of medicines with new indications that use the total polysaccharide of Platycodon grandiflorum as an effective ingredient.

The invention discloses application of anthocyanin in preparation of drugs for treating cell injury caused by arsanilic acid. An in-vitro chick embryo fibroblast DF-1 cell model is established for studying application of arsanilic acid and anthocyanin in apoptosis of DF-1 cells. The application of arsanilic acid and the anthocyanin in apoptosis of the DF-1 cells firstly discovers that arsanilic acid has cytotoxicity on the DF-1 cells and is capable of inducing the apoptosis. The anthocyanin with a certain concentration being 50-100 micrograms per milliliter can resist the apoptosis caused by arsanilic acid; the medical application of the anthocyanin is expanded; the development of drugs taking the anthocyanin as an effective component and having new indications can be facilitated.