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Brefeldin A-producing bacteria and method for preparing brefeldin A by fermentation

A technology for producing bacteria and fermentation products, applied in the directions of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as methods for which BrefeldinA has not yet been discovered, and achieve significant social benefits.

Inactive Publication Date: 2009-11-18
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0100] Through literature search, etc., so far, no new Brefeldin A producing bacteria Mortierella monospora and the method for preparing Brefeldin A by fermentation of the producing bacteria have been found.

Method used

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  • Brefeldin A-producing bacteria and method for preparing brefeldin A by fermentation
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  • Brefeldin A-producing bacteria and method for preparing brefeldin A by fermentation

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preparation example Construction

[0233] (1) Preparation method of fungi and fermentation products thereof

[0234] In order to further illustrate the preparation method of the above-mentioned Brefeldin A producing bacterial strain and its fermentation product Brefeldin A, the specific operation steps and methods are illustrated below by way of example.

[0235] Taking garden ginseng as an example, the specific preparation method of the Brefeldin A producing strain is as follows:

[0236] (1) Select fresh and healthy garden ginseng, remove ginseng whiskers and reed heads, wash the surface of the root, dry the water, and sterilize;

[0237] The concrete operating steps of described disinfection method are:

[0238] ① Rinse with 75% ethanol for 20-30 seconds in a sterile operating table;

[0239] ② Rinse with sterile water 4 times;

[0240] ③ Rinse with 5% NaClO solution for 5 minutes;

[0241] ④ Rinse with sterile water 4 times;

[0242] ⑤ Rinse with 75% ethanol for 20-30 seconds;

[0243] ⑥ Rinse with st...

Embodiment 1

[0272] Embodiment 1, the preparation method one of fermentation product Brefeldin A

[0273] Use PDA (glucose 1%, sucrose 1%, potato 20%, agar 1%, pH natural) slant medium, sterilize at 120 ℃ for 30 minutes, make slant, place it at 37 ℃ at room temperature for 24 hours, no impurities When the fungus grows, inoculate the mycelia of Mortierella monospora strain, and culture at 28° C. for 7 days. 80 ml of liquid culture medium (glucose 1%, sucrose 1%, potato 20%, pH natural) were filled in multiple 250 ml Erlenmeyer flasks respectively. After sterilizing at 120° C. for 30 minutes, inoculation was performed, followed by shaking culture at 28° C. for 48 hours, and the culture was used as a seed. Prepare a 5000mL fermentation bottle with a fermentation medium capacity of 2000mL, the medium composition is the same as the seed medium, sterilized at 120°C for 30 minutes, the inoculum size is 8%, 28°C, and 180 rpm shaking culture for 7 days. The fermentation broth and mycelium were co...

Embodiment 2

[0274] Embodiment 2, the preparation method two of fermentation product Brefeldin A

[0275] Use PDA (glucose 1%, sucrose 2%, potato 20%, agar 1%, pH natural) slant medium, sterilize at 120 ℃ for 30 minutes, make a slant, place it at 37 ℃ for 24 hours at normal temperature, no impurities When the fungus grows, inoculate the mycelia of Mortierella monospora strain, and culture at 28° C. for 7 days. 80 ml of liquid culture medium (2% sucrose, 20% potato, natural pH) were filled in multiple 250 ml Erlenmeyer flasks respectively. After sterilizing at 120° C. for 30 minutes, inoculation was performed, followed by shaking culture at 28° C. for 48 hours, and the culture was used as a seed. Prepare a 5000mL fermentation bottle with a fermentation medium capacity of 2000mL, the medium composition is the same as the seed medium, sterilized at 120°C for 30 minutes, the inoculum size is 8%, 28°C, and 180 rpm shaking culture for 7 days. The fermentation broth and mycelium were collected ...

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Abstract

The invention relates to Brefeldin A-producing bacteria, a preparation method, a method for preparing the brefeldin A by fermentation and the application thereof. The Brefeldin A-producing bacteria has the advantages of easy selection, cultivation and storage, low cost, simple process, short preparation period, high yield, no usage of toxic raw material, less organic solvent, thorough guarantee of medicine resource, potential of mass production and favorable economic and social benefits, contributes to the maintenance of ecological balance and the protection of natural resources. The fermentation product of the invention, of which the main component is THSG, develops novel medical application, the Brefeldin A has high safety, low toxicity, stable property, stronger pharmacological action, simple preparation process, high yield and considerably wide range of application, is multipurpose, and can provide a novel source for medicines for preventing, diagnosing, detecting, protecting, curing and researching antimycotic, antiviral and antineoplastic diseases and the directly related diseases.

Description

technical field [0001] The present invention relates to the technical fields of medicine and food, in particular to a preparation method of a fungus and a fermentation product thereof, that is, a preparation method of an endophytic fungus of the genus Mortierella Coemans and a fermentation product thereof, more specifically to The invention discloses a preparation method of Mortierella monospora Linnemann and its fermentation product Brefeldin A. Background technique [0002] (1) Research overview of Brefeldin A [0003] 1 Overview [0004] Brefeldin A (English name: Brefeldin A, abbreviated as: BFA, Chinese name or: Brefeldin A, also written: Brefeldin A, also known as clindamycin or cyanobacteria Ascorine or ascorine), a macrolide antibiotic, was first isolated from Penicillium brefeldianum in 1958. [0005] Brefeldin A has a variety of biological activities, such as antifungal, antiviral, antitumor, antinematode and so on. Brefeldin A is a widely used protein transpor...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/08A61K36/06A61K31/365A61P31/10A61P31/12A61P35/00C12Q1/18A23L1/28C12R1/645A23L31/00
Inventor 秦路平徐丽莉韩婷黄宝康
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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