Application of platycodon grandiflorum(Jacq)A .DC polysaccharide for preparing medicine capable of treating cellular damage caused by Cr(VI)

A technology of platycodon grandiflorum polysaccharide and cell damage, applied in the application field of medicine, can solve the problems such as cell damage of platycodon grandiflorum polysaccharide which have not yet been seen

Pending Publication Date: 2019-12-20
山东鑫谷健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the effect of Platycodon grandiflorum on antagonizing cell damage caused by Cr(VI)

Method used

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  • Application of platycodon grandiflorum(Jacq)A .DC polysaccharide for preparing medicine capable of treating cellular damage caused by Cr(VI)
  • Application of platycodon grandiflorum(Jacq)A .DC polysaccharide for preparing medicine capable of treating cellular damage caused by Cr(VI)
  • Application of platycodon grandiflorum(Jacq)A .DC polysaccharide for preparing medicine capable of treating cellular damage caused by Cr(VI)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation of bellflower polysaccharide

[0028] Take platycodon root decoction pieces, add 10 times the weight of water to soak overnight, then extract at 80°C for 4 hours, filter, concentrate the filtrate to a relative density of 1.05-1.15 (measured at 50°C), and centrifuge at 3000rpm for 10min to remove impurities. obtain the extract;

[0029] Add ethanol with a volume concentration of 95% to the extract, stir while adding, so that the final concentration of ethanol is 60%, let it stand for 24 hours, centrifuge, separate the precipitate, and dry the precipitate in vacuum at 60°C to obtain PGPS 60c .

[0030] Add ethanol with a volume concentration of 95% to the supernatant after separation and precipitation, stir while adding, so that the final concentration of ethanol is 80%, let stand, centrifuge, separate the precipitate, and dry the precipitate in vacuum at 60°C to obtain PGPS 80c .

[0031] PGPS 60c and PGPS 80c Mix according to the weigh...

Embodiment 2

[0032] Example 2: Investigation of the proliferation effect of bellflower polysaccharides on DF-1 cells

[0033] 1. Test method:

[0034] In order to study the influence of bellflower polysaccharides on DF-1 cells, the bellflower polysaccharides prepared in Example 1 were formulated into solutions of different concentrations, and the DF-1 cells were treated with different concentrations of bellflower polysaccharides for 12 hours, and the changes in cell viability were detected.

[0035] 2. Test results:

[0036] See the test results figure 1 ,Depend on figure 1 It can be seen that when the concentration is lower than 400 μg / ml, the platycodon polysaccharide prepared in Example 1 has no toxic effect on cells.

Embodiment 3

[0037] Example 3: Study on the protective effect of bellflower polysaccharides on DF-1 cell damage caused by Cr(VI)

[0038] 1. Test method:

[0039] The protective effect of Platycodon grandiflora polysaccharide on DF-1 cell injury caused by Cr(VI) was studied by CCK-8 method to detect cell viability.

[0040] Culture DF-1, and when the cell fusion degree reaches more than 80%, the cells (5×10 5 cells / well) to a 96-well plate (100 μL per well) and cultured for 24 h. The experiment was divided into five treatments, namely: Con group, Cr group, treatment group 1, treatment group 2 and treatment group 3; among them, the Con group was the blank control without treatment; the Cr group was treated with 40 μM Cr(VI) Cells for 6h; treatment group 1 with 40μM Cr(VI) and 200μg / ml PGPS 60c Treat cells for 6h; treat 2 groups with 40μM Cr(VI) and 200μg / ml PGPS 80c Treat the cells for 6h; treat 3 groups with 40μM Cr(VI) and 200μg / ml platycodon polysaccharide (PGPS 60c and PGPS 80c Ac...

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Abstract

The invention discloses an application of platycodon grandiflorum(Jacq)A .DC polysaccharide for preparing a medicine capable of treating cellular damage caused by Cr(VI). A model for chicken embryo fibroblast line (DF-1 cell) cellular damage caused by Cr(VI) is established to research the function of the platycodon grandiflorum(Jacq)A .DC polysaccharide in the cellular damage. The invention discovers for the first time that the platycodon grandiflorum(Jacq)A .DC polysaccharide of a certain concentration (50-200[mu]g/ml) can resist the cellular damage caused by Cr(VI). The medical application of the platycodon grandiflorum(Jacq)A .DC polysaccharide is widened, and the invention is favorable for developing medicines which take the platycodon grandiflorum(Jacq)A .DC polysaccharide as an effective ingredient and have new indications.

Description

technical field [0001] The invention relates to the field of medical applications of platycodon polysaccharides, in particular to the application of platycodon polysaccharides in the preparation of medicines for treating cell damage caused by Cr(VI). Background technique [0002] Chromium (Cr) is a common constituent of organic substances and is ubiquitous in the natural environment. Chromium has a variety of applications in industry, including chemical, refractory and metallurgical industries. Its valence ranges from -2 to +6, with trivalent chromium and hexavalent chromium being the most common valence states. Of all the valence states, hexavalent chromium is most readily absorbed. According to reports, the retention of hexavalent chromium salts in rats is ten times that of trivalent chromium salts. After hexavalent chromium ions enter the blood, they quickly pass through the red blood cell membrane and combine with globin in hemoglobin. Under conditions of success, ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P39/02A61P43/00
CPCA61K31/715A61P39/02A61P43/00
Inventor 刘建柱郝佳佳刘永夏邓干臻李克鑫李克钦李国保王振峰
Owner 山东鑫谷健康产业有限公司
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