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Application of Edo sakuranin to treatment of Alzheimer

A technology of sakura and glucoside, which is applied in the field of biomedicine, can solve the problems of prone to breast cancer and uterine cancer, endanger the clinical application of estrogen, etc., and achieve the effects of strong pharmacological effects, wide application range, and simple preparation process

Active Publication Date: 2018-10-16
HEILONGJIANG UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, estrogen may predispose women to breast and uterine cancer, which undoubtedly jeopardizes the clinical application of estrogen

Method used

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  • Application of Edo sakuranin to treatment of Alzheimer
  • Application of Edo sakuranin to treatment of Alzheimer
  • Application of Edo sakuranin to treatment of Alzheimer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 MTT method detects estradiol (E 2 ) to Aβ 25-35 Effect on Viability of PC-12 Cells

[0036] 1. Cell culture

[0037] Rat adrenal pheochromocytoma cell line PC-12 (BIOSYNTHESIS BIOTECHNOLOGY CO., LTD), with DMEM medium containing 10% FBS and 1% P / S, at 37 ° C, 5% CO 2 Culture in an incubator, change the medium once every 2-3 days, use 0.25% EDTA-containing trypsin for routine digestion and passaging, and take the cells in the logarithmic growth phase for the experiment.

[0038] 2. Grouping

[0039] 1)E 2 Safe Concentration Screening Group:

[0040] Blank group: after 24 hours of culture in DMEM culture medium, the DMEM culture medium was replaced once, and the culture was continued for 24 hours;

[0041] E. 2 Group: After 24 hours of culture in DMEM medium, the final concentrations were 10 μmol / L, 1 μmol / L, and 10 μmol / L, respectively. -1 μmol / L, 10 -2 μmol / L, 10 -3 μmol / L, 10 -4 μmol / L, 10 -5 μmol / L of E 2 The solution was continued to cultiva...

Embodiment 2

[0062] Example 2 MTT method detection of edo cherry blossom glycosides on Aβ 25-35 Effect on Viability of PC-12 Cells

[0063] 1. Cell culture steps are the same as in Example 1

[0064] 2. Grouping

[0065] 1) Edo cherry blossom glycoside safety concentration screening group:

[0066] Blank group: after 24 hours of culture in DMEM culture medium, the DMEM culture medium was replaced once, and the culture was continued for 24 hours;

[0067] Edo sakura glycoside group: After 24 hours of culture in DMEM medium, the final concentration was replaced by 4×10 2 μmol / L, 40μmol / L, 4μmol / L, 4×10 -1 μmol / L, 4×10 -2 μmol / L, 4×10 -3 μmol / L, 4×10 -4 μmol / L, 4×10 -5 The edo pakura glycoside solution of μmol / L continued to cultivate for 24h;

[0068] 2) Edo cherry blossom glycoside effective concentration screening group:

[0069] Blank group: after 24 hours of culture in DMEM culture medium, the DMEM culture medium was replaced once, and the culture was continued for 26 hours;

...

Embodiment 3

[0084] Example 3 Edo Sakuraside on Aβ 25-35 Study on the Protective Effect of Induced PC12 Cell Injury

[0085] 1. Cell culture steps are the same as in Example 1

[0086] 2. Grouping

[0087] Blank group: after 24 hours of culture in DMEM culture medium, the DMEM culture medium was replaced once, and the culture was continued for 26 hours;

[0088] Model group: cultured in DMEM medium for 24 hours, replaced with DMEM medium and cultured for 2 hours, then given Aβ 25-35 Solution, so that the final concentration is 20 μmol / L, continue to cultivate for 24h;

[0089] E2+Aβ 25-35 Group: cultured in DMEM medium for 24 hours, replaced with a final concentration of 10 -3 μmol / L of E 2 Solution cultured for 2h, given Aβ 25-35 Solution, so that the final concentration is 20 μmol / L, continue to cultivate for 24h;

[0090] Edo Sakuraside + Aβ 25-35 Group: cultured in DMEM medium for 24 hours, replaced with a final concentration of 4×10 -1 Cultured with μmol / L edo sakura glycosi...

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Abstract

The invention discloses application of Edo sakuranin to treatment of Alzheimer. The application has the advantages that experiments prove that the Edo sakuranin can promote the proliferation rate andactivity of a PC-12 cell damaged by A Beta 25-35 and meanwhile can inhibit apoptosis of the PC-12 cell damaged by A Beta 25-35, and a novel method for the treatment of Alzheimer is provided.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of edo sakura glycoside in the treatment of Alzheimer's disease. Background technique [0002] Alzheimer's Disease (AD), also known as senile dementia, is a neurodegenerative disease that occurs in old age and is characterized by chronic irreversible memory decline and cognitive function blockage. It usually takes ten years to die. The disease seriously endangers people's health and brings countless emotional and economic burdens to families and society. With the improvement of people's living standards in our country, the average life expectancy of the population is extended, and aging has become an important problem that our country is currently facing. AD will become a serious problem that affects people's quality of life and quality of life. There is currently no effective cure for AD, and no success has been found in more than 100 human trials of AD treatments conduc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P25/28A61P25/00
CPCA61K31/7048A61P25/00A61P25/28A61K2300/00
Inventor 张宁耿放王发善雷霞刘斌刘海洋薛慧杨柳
Owner HEILONGJIANG UNIV OF CHINESE MEDICINE
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