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Antineoplastic traditional Chinese medicine preparation and preparation method thereof

A traditional Chinese medicine product and anti-tumor technology, which is applied in the fields of medicine, food, and beverage, can solve the problems of high price, poor water solubility, and limited clinical application, and achieve the effect of improving the therapeutic index

Inactive Publication Date: 2009-01-14
中国人民解放军第四五四医院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although betulinic acid has anti-tumor effects, the lack of betulinic acid (less amount, high price, 882 yuan for 25 mg) directly affects its development and research as a clinical drug; on the other hand, betulinic acid itself As a clinical drug, there is a weakness of poor water solubility, which also limits its clinical application

Method used

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  • Antineoplastic traditional Chinese medicine preparation and preparation method thereof
  • Antineoplastic traditional Chinese medicine preparation and preparation method thereof
  • Antineoplastic traditional Chinese medicine preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Preparation of betulinic acid liposome powder injection

[0050] 1. Preparation method of betulinic acid liposome powder injection

[0051] (1) Preparation of betulinic acid liposome powder injection

[0052] Betulinic acid 0.15g

[0053] Lecithin 0.15g

[0054] Cholesterol 0.15g

[0055] Mannitol 0.30g

[0056] Thin film method (TFV): Weigh 0.15g betulinic acid, 0.15g lecithin and 0.15g cholesterol into a pear-shaped bottle, add 10ml ethanol, shake, add several glass beads, and place on a rotary evaporator at 28~32℃ Evaporate to dry to form a film, vacuum dry until there is no ethanol smell in the pear-shaped bottle, add 7-15ml of phosphate buffered saline solution, shake the film off, sonicate for 10-30min in an ultrasonic instrument to dissolve the film, and pass through a 0.45μm filter membrane , A colorless and transparent liquid is obtained, and the drug encapsulation rate is over 90%. Weigh the prescription amount of mannitol, dissolve it with 15ml of hydr...

Embodiment 2

[0087] Example 2. The effect of betulinic acid and its liposomes on human melanoma cell line A375

[0088] 1 Materials and methods

[0089] 1.1 Drug: betulinic acid liposome was prepared according to Example 1.

[0090] 1.2 Tumor line: Human melanoma cell line A375 was provided by the Chinese Academy of Sciences Cell.

[0091] 1.3 Reagents and equipment: MTT (purchased from Sigma); DMEM, trypsin, calf serum (purchased from GIBCO). Multiskan MK3 microplate reader (Labsystems Dragon)

[0092] 1.4 MTT method to measure the inhibition rate of cell proliferation: collect the human melanoma cell line A375 (2×104 / ml) in logarithmic growth phase, plant them into 96-well culture plate, 100μl per well, add the drug solution according to the design, negative control Use the corresponding culture medium instead, place it in an incubator with 5% CO2 and 37°C for 24 hours. After the treated cells, remove the DMEM medium, wash twice with D-Hank's solution, add 100 μl DMEM medium and 10 μl MTT (5...

Embodiment 3

[0098] Example 3. Effect of betulinic acid liposomes on transplanted tumor of mouse colon cancer cell line (C26)

[0099] 1 Materials and methods

[0100] 1.1 Experimental animals: The mouse colon cancer cell line C26 was provided by Shanghai Medical Engineering Institute; BABL / C+ICR=F1 mice, 19-22 grams, purchased from Shanghai Slack Laboratory Animal Co., Ltd., license number: SCXK (Shanghai) 2003-0003.

[0101] 1.2 Reagents and instruments: cyclophosphamide (specification: 200mg / bottle, batch number: 050206, Shanghai Hualian Pharmaceutical Co., Ltd.); balance (JA2003A, Shanghai Jingtian Electronic Instrument Co., Ltd.)

[0102] 1.3 Establishment of transplanted tumor model of mouse colon cancer (C26) and drug effects Select vigorously growing mouse colon cancer (C26) tumor source, prepare cell suspension by homogenization method (1:8), each mouse is right Inoculate 0.2ml under the axillary skin. At the same time, betulinic acid liposomes (1mg / kg, 10mg / kg) were injected intraper...

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Abstract

An anti-tumor Chinese medicine product comprises medicine, reagent, food and drink and is characterized in that the effective component of the Chinese medicine product is a betulic acid liposome containing betulic acid. The entrapment rate of the betulic acid liposome reaches over 90 percent and particle diameter is 100 to 1000 nanometers. Firstly, the betulic acid plastid is obtained by membrane process; and then the single betulic acid liposome or the betulic acid liposome as well as other chemical substances comprising a pharmaceutically acceptable carrier, the food, a natural product, synthetic chemicals or medicine composition for human are made into the medicine, the food or the drink by general process.

Description

Technical field [0001] The invention relates to a traditional Chinese medicine product, in particular to an anti-malignant traditional Chinese medicine product and a preparation method thereof, and belongs to the technical fields of medicine, food and beverage. Background technique [0002] In the past 10 years, cancer has quietly risen to the second leading "killer disease" in humans (the death rate is second only to cardiovascular disease), while the infectious diseases that once ravaged the world have retreated to the third place. According to analysis, the sharp increase in cancer incidence is related to increased environmental pollution, unscientific eating habits and unhealthy lifestyles. The rising prevalence of cancer has prompted the continuous expansion of the anti-cancer drug market. In the past few years, the total sales of various anti-cancer drugs have been rising rapidly. Take the US anti-cancer drug market as an example: In 1999, the total sales of 10 major anti-c...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61K9/127A61K47/24A61P35/00
Inventor 陆瑜杨春华邱彦刘静涵赵伯诚朱昌罗司梁宏黄大超
Owner 中国人民解放军第四五四医院
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