Patrinias cabra aglycon extraction and its preparation method and anti colon cancer application

A technology of sapogenin and extraction method, which is applied in the fields of beverage, food and medicine, can solve the problems such as the effect of preventing and treating colon cancer of the extract of S.

Inactive Publication Date: 2007-10-24
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0085] Through literature search, etc., so far, no report has been found that the

Method used

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  • Patrinias cabra aglycon extraction and its preparation method and anti colon cancer application
  • Patrinias cabra aglycon extraction and its preparation method and anti colon cancer application
  • Patrinias cabra aglycon extraction and its preparation method and anti colon cancer application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0239] Embodiment 1, the preparation method of patensin extract

[0240] (1) Preparation of Patratenin Extract

[0241] 50 kilograms of dry rhizomes of Palladium palatinea are crushed, moistened with methanol, put into a percolation barrel, and percolated with methanol. Concentrate under reduced pressure at 50°C, add hot water at about 50°C, and suspend. Extract with petroleum ether (60-90). A petroleum ether layer and an aqueous layer were obtained. The petroleum ether layer was concentrated to obtain a partial extract of petroleum ether. 200ml of the water layer was extracted with ethyl acetate and n-butanol in sequence, and recovered under reduced pressure to obtain the ethyl acetate part and the n-butanol part.

[0242] Take 250g of ethyl acetate extract, dissolve it with chloroform-methanol 10:1, mix with 500g of silica gel (100-200 mesh), and evaporate to dryness at 60°C. Pack a silica gel column, and the silica gel used for packing the column is 2000g. Elute with ...

Embodiment 2

[0253] Example 2, the effect of patron aglycone extract and monomer (PS-1, BJ3401) on mouse colon cancer cell line C26

[0254] 1 Materials and methods

[0255] 1.1 Drugs: the extract of Patrine aglycon was extracted with reference to Example 1, PS-I and BJ3401 were the monomers contained therein, and were separated and prepared from the extract of Patrine aglycon by HPLC.

[0256] 1.2 Tumor strain: mouse colon cancer cell line C26 provided by Shanghai Medical Engineering Institute

[0257] 1.3 Reagents and instruments: MTT (purchased from Sigma); DMEM, trypsin, calf serum (purchased from GIBCO). Multiskan MK3 microplate reader (Labsystems Dragon company)

[0258] Table 2. The effect of iridoid aglycone (PS-I, BJ3401) in Patrinia palatinae on the growth of mouse colon cancer cell line C26

[0259] group

Concentration (mg / ml)

C26

A

Inhibition rate(%)

control group

-

1.132±0.063

- ...

Embodiment 3

[0265] Embodiment 3, the influence of the Patina aglycone extract in mice colon cancer (C26) transplantation tumor

[0266] 1 Materials and methods

[0267] 1.1 Experimental animals: mouse colon cancer cell line C26 provided by Shanghai Medical Engineering Institute; BABL / C+ICR=F1 mice, 19-22 grams, purchased from Shanghai Slack Experimental Animal Co., Ltd., license number: SCXK (Shanghai) 2003-0003.

[0268] 1.2 Reagents and instruments: cyclophosphamide (specification: 200mg / bottle, batch number: 050206, Shanghai Hualian Pharmaceutical Co., Ltd.); balance (JA2003A, Shanghai Jingtian Electronic Instrument Co., Ltd.)

[0269] 1.3 Establishment of mouse colon cancer (C26) transplanted tumor model and drug effect: select the vigorously growing mouse colon cancer (C26) tumor source, prepare cell suspension by homogenate method (1:8), and each mouse Inoculate 0.2ml subcutaneously in the right armpit. At the same time, the extracts of patriginin (200 mg / kg, 800 mg / kg) were admi...

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Abstract

The invention relates to a medicine treating carcinoma of colon and the method for preparing the same, which in detail is aglycone extract from root of Patrinia scabra Bge and the preparation method. The effective component is iridoid glycosides aglycone, the content is above 40%. The invention also provides the medicine agent and preparation. It is proved by pharmacologia test that the medicine is characterized by safe utilization, function of preventing the weight of tumor in transplanted carcinoma of colon. The extract can be produced into preparations of tablet, capsule, drop pill, granular or oral.

Description

technical field [0001] The present invention relates to the technical fields of medicine, food and beverage, in particular to extracts of traditional Chinese medicines and their preparation methods and applications, and more specifically to extracts of patinogenin from Chinese medicines Uses for colon cancer products. Background technique [0002] (1) General situation of the research on C. [0003] 1. Overview of plant resources and applications [0004] Patrinia scabra Bunge. is a plant of Patriniaceae Patrinia genus, a perennial herb with a height of 20-40 cm. The stems are clustered, and the upper part of the stem is multi-branched, with knotted lines on the branches. Leaves opposite; leathery, pinnately divided, lobes oblanceolate, narrowly lanceolate or oblong, with teeth, apical lobes slightly larger than lateral lobes, leaf margins and leaf surfaces are hairy. The cymes are terminal and arranged in a corymb shape; fine hairs grow on the rachis and pedicel; the br...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P35/00
Inventor 李铁军顾正兵邱彦毛俊琴陈强芮耀诚
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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