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Composition for resisting ischemia reperfusion injury and preparation method and application thereof

A reperfusion injury and composition technology, applied in the field of anti-ischemia-reperfusion injury composition and its preparation and use, can solve the problems such as preparation method and use of anti-ischemia-reperfusion injury products that have not yet been found

Inactive Publication Date: 2012-07-18
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0118] Through literature search, etc., so far, no new anti-ischemia-reperfusion injury products, their preparation methods and uses have been found.

Method used

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  • Composition for resisting ischemia reperfusion injury and preparation method and application thereof
  • Composition for resisting ischemia reperfusion injury and preparation method and application thereof
  • Composition for resisting ischemia reperfusion injury and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0569] Example 1. Expression of α7nAChR in mouse liver tissue

[0570] The expression of α7nAChR in the liver tissue of male C57BL / 6 mice was detected by western-blotting method, the mice were sacrificed by dislocation of the cervical spine, the liver tissue was homogenized, the total protein was extracted, and the expression of α7nAChR in the liver tissue was detected, and normal mouse liver tissue was found have α7nAChR expression (see figure 1 ).

Embodiment 2

[0571] Example 2. Changes in liver blood flow during mouse liver ischemia-reperfusion

[0572]C57BL / 6 mice were selected, and the blood flow in the left middle lobe of the liver (hepatic blood flow HBF) was monitored in real time by Doppler flowmeter during partial ischemia-reperfusion surgery. to 32%±0.049, the blood flow stabilized at 17%±0.032 of normal blood flow after 10 minutes of ischemia, the blood flow recovered to 66%±0.112 immediately after the arterial clamp was removed, and the blood flow completely recovered to normal level after 10 minutes (see figure 2 ).

Embodiment 3

[0573] Example 3. Effects of Different Doses of Atr.+Neo. Combined Therapy on Ischemia-Reperfusion Injury

[0574] Taking partial ischemia-reperfusion of the mouse liver as a model, 35 male C57BL / 6 mice (22±2.0g) were randomly divided into five groups, and one group was sutured immediately after laparotomy. The other four groups were injected intraperitoneally with normal saline solution (10ml / kg), PNU-282987 (40μg / kg), Atr.(0.2mg / kg)+Neo.(0.4mg / kg), Atr.(0.1mg / kg) +Neo.(0.2mg / kg), liver ischemia treatment was performed 30min after drug injection, reperfusion was started at 60min after ischemia, and liver tissue was collected at 6h after reperfusion, and the livers of animals in each group were detected by enzyme-linked immunosorbent assay (ELISA) technology Caspase-3, 8, 9 activity (see image 3 ).

[0575] The results showed that: compared with the normal control group, the activity of Caspase-3, 8, and 9 in the model group was significantly increased ( ** P*** P<0.001, n...

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PUM

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Abstract

The invention relates to a medicinal composition for treating an ischemia reperfusion injury, particularly a hepatic ischemia reperfusion injury, and a preparation method and application thereof. The composition comprises an M-cholinergic receptor blocker and a cholinesterase inhibitor, has a simple and convenient preparation process, is safe to use, has an obvious curative effect, can be used for obviously improving the cell apoptosis caused by the ischemia reperfusion injury and overcomes the side effects caused by existing common medicaments. The invention expands novel medical application of an existing muscarinic receptor blocker and also provides a novel medicine intervention means for preventing and treating the ischemia reperfusion injury. The medicinal composition is suitable for large-scale production and commercial application in the industries of medicine, reagents and the like, has excellent application prospect and has obvious social benefits and economic benefits.

Description

technical field [0001] The present invention relates to technical fields such as medicine and reagents, in particular to a pharmaceutical composition and its preparation method and use, and more specifically to a drug for treating ischemia-reperfusion injury, especially hepatic ischemia-reperfusion injury. The pharmaceutical composition for perfusion injury, its preparation method and application, more specifically relates to a pharmaceutical composition containing M choline receptor blocker and cholinesterase inhibitor, its preparation method and application. Background technique [0002] (1) Research overview of ischemia-reperfusion injury [0003] 1 Overview [0004] After the ischemic tissues and organs get blood supply again, not only the function of the tissues and organs cannot be restored, but the dysfunction of function and metabolism and the destruction of structure are aggravated. This phenomenon is called ischemia-reperfusion injury (ischemia-reperfusion injury...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/06A61K31/46A61K31/407A61P1/16A61K31/27
Inventor 沈甫明倪敏赵婷杨中伟苏定冯
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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