Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Simple monascus solid separation method

A technology for solid-state separation and monascus, applied in the field of microbial separation, can solve the problems of no microbial enrichment, slow growth, and no consideration of the optimal selection of enrichment medium, so as to reduce the interference of miscellaneous bacteria and improve the enrichment effect. , the effect of enrichment for a long time

Inactive Publication Date: 2019-09-13
福建生物工程职业技术学院
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above method is to directly separate the materials to be separated by different methods, without enriching microorganisms, and without considering the optimization of the enrichment medium, nor considering that in the mixed bacteria, if the target strain The growth is slow and will be inhibited by other microorganisms with dominant growth. When using the above method to isolate Monascus from mixed bacteria, Monascus may not be isolated at all.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Simple monascus solid separation method
  • Simple monascus solid separation method
  • Simple monascus solid separation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A simple solid-state isolation method for Monascus, comprising the following steps:

[0037] (1) The configuration of the culture medium. Because the existing commercialized dehydration culture medium and other culture media are difficult to enrich Monascus, therefore, the present invention has made a nutrient-rich malt juice agar medium that can enrich Monascus in a large amount, and the advantages of self-made culture medium It is rich in nutrients and is more conducive to the growth of microorganisms.

[0038] The preparation method of malt juice agar medium comprises the following steps:

[0039] 1) Take some barley, wash it with water, soak in water for 6 hours, germinate in a dark place at 15°C, cover it with a piece of gauze, and pour water once a day in the morning, noon, and evening. When the malt stretches to twice the size of the grain, it stops Germinate, spread out to dry or dry, and store for later use. 2) Grind the dry malt into malt powder, by weight,...

Embodiment 2

[0048] (1) The configuration of the culture medium: because the existing commercial dehydrated culture medium and other culture medium are difficult to enrich Monascus, therefore, the present invention self-made nutrient-rich malt juice agar capable of enriching Monascus in large quantities Medium.

[0049] The preparation method of malt juice agar medium comprises the following steps:

[0050] 1) Take some wheat, wash it with water, soak in water for 12 hours, germinate in a dark place at 15°C, cover it with a piece of gauze, and pour water once a day in the morning, noon, and evening. When the malt stretches to twice the size of the wheat grain, it stops Germinate, spread out to dry or dry, and store for later use. 2) Grind the dry malt into malt powder, by weight, add one part of malt powder to four parts of water, saccharify in a water bath at 65°C for 4 hours, and titrate the degree of saccharification with iodine until it becomes colorless. 3) Filter the saccharificati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a simple monascus solid separation method and belongs to the technical field of microbial separation. The method comprises five steps as follows: preparation of a culture medium, addition of an inhibitor, enrichment culture, marking separation and dibbling. The self-made malt agar culture medium containing an inhibitor is used for efficient solid enrichment of monascus in red kojic rice and separation and purification. The separation method is convenient to operate, high in separation speed and good in separation effect.

Description

technical field [0001] The invention belongs to the technical field of microorganism separation, and in particular relates to a simple solid-state separation method of Monascus bacterium. Background technique [0002] Existing methods for separating Monascus bacterium from mixed bacteria with solid medium include dilution pouring plate method, coating plate method, and plate streaking method. [0003] The method of diluting the plate is to make the mixed bacteria into a bacterial suspension, and then perform 10-fold incremental dilution, and then take a little of the bacterial solution of different dilutions, add it to the plate, pour the agar medium at about 50°C to mix, and shake well. After the agar has solidified, it is incubated for a certain period of time, and colonies will appear. When the dilution is appropriate, scattered single colonies appear on the plate after incubation. These single colonies may be formed by the multiplication of a single bacterial cell. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02C12N1/14C12R1/645
CPCC12N1/02C12N1/14
Inventor 孙丽娟陈梁军陈秉梅
Owner 福建生物工程职业技术学院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com