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Peripheral blood transcription gene marker for breast cancer detection and application thereof

A technology of breast cancer and characteristic genes, applied in the field of molecular biology, can solve problems such as women's quality of life and even psychological harm, and achieve the effects of early detection, high sensitivity and specificity, and safe sampling

Active Publication Date: 2019-09-17
北京纳昌珠宝商行有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the mammary gland is an important sexual characteristic of women, although breast cancer surgery is a good treatment, it will seriously harm women's quality of life and even their psychology.

Method used

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  • Peripheral blood transcription gene marker for breast cancer detection and application thereof
  • Peripheral blood transcription gene marker for breast cancer detection and application thereof
  • Peripheral blood transcription gene marker for breast cancer detection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Screening of peripheral blood characteristic gene markers of breast cancer

[0036] The screening of peripheral blood characteristic genes of breast cancer includes the following steps:

[0037] 1) Using PAXgene TM The Blood RNA Tube blood collection tube collects peripheral blood samples from 55 cases of breast cancer patients confirmed by pathological examination and 50 cases of normal people, and each sample collects 2-3mL peripheral blood.

[0038] 2) The total RNA of the above peripheral blood samples was extracted using the PAXgene Blood RNA Kit extraction kit. Agilent Bioanalyzer 2100 bioanalyzer was used to detect the fragment integrity (RIN) of RNA samples, and the purity of RNA samples was detected by Nano1000 micro-ultraviolet spectrophotometer. All RNA samples must meet the following quality control conditions: RNA yield greater than 2 micrograms, 28S / 18S peak ratio greater than 1, RIN value greater than 7, and 260nm / 280nm absorbance ratio greate...

Embodiment 2

[0050] Example 2 Detection of Breast Cancer Using Screened Breast Cancer Peripheral Blood Characteristic Gene Markers

[0051] 1. Method steps:

[0052] 1) Collection of peripheral blood samples of samples to be tested: using PAXgene TM Blood RNA Tube blood collection tube collects 2ml of peripheral blood from the subject's cubital vein;

[0053] 2) Extraction and purification of total RNA in the peripheral blood samples of the samples to be tested: use the PAXgene Blood RNA Kit extraction kit to extract and purify the total RNA in the peripheral blood, and use the Agilent BioAnalyzer 2100 microelectrophoresis analyzer to identify the integrity of the extracted total RNA fragments and yield, the purity of the RNA sample was detected with a Nano1000 micro-volume ultraviolet spectrophotometer;

[0054] 3) Reverse transcription reaction: use Life Techonolgy's High-Capacity cDNA ReverseTranscription kit reverse transcription kit, use total RNA as a template, and use Olig(dT) as ...

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Abstract

The invention discloses a peripheral blood transcription gene marker for breast cancer detection. The peripheral blood transcription gene marker comprises blood cell characteristic gene sequences of breast cancers shown in SEQ ID NO.1-SEQ ID NO.10. The invention also discloses application of the gene marker in preparation of breast cancer detection products. According to the peripheral blood transcription gene marker, early screening detection is carried out on the breast cancers through combinatory analysis on the expression conditions of transcription gene markers related to the breast cancers in peripheral blood. The peripheral blood transcription gene marker has the advantages of high sensitivity and strong specificity, the peripheral blood which is most easily collected clinically is taken as a detection sample, sampling is safe, simple and convenient, the peripheral blood transcription gene marker is very suitable for early screening and auxiliary diagnosis of the breast cancers of a large-scale population, and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a blood cell transcriptional gene marker for detecting breast cancer and its application. Background technique [0002] Breast cancer is a common malignant tumor in adult women and one of the leading causes of cancer death in adult women. Breast cancer accounts for the first new incidence rate and the second mortality rate among female malignant tumors in the world. There are about 79,000 new cases every year, accounting for 21% of all female malignant tumors. The annual average incidence rate of breast cancer in the world is 30.30 / 100,000, and the world population adjustment rate (world adjustment rate) is 32.97 / 100,000. Nearly 60% of new breast cancer cases occur in developed countries. In developed countries, the number of new cases of breast cancer is about 25% of all malignant tumors in women. The incidence rate was 79.09 / 100,000, and the adjusted rate was 56.4...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11C40B40/06
CPCC12Q1/6886C40B40/06C12Q2600/158
Inventor 程昌明
Owner 北京纳昌珠宝商行有限公司
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