Peripheral blood transcription gene marker for breast cancer detection and application thereof
A technology of breast cancer and characteristic genes, applied in the field of molecular biology, can solve problems such as women's quality of life and even psychological harm, and achieve the effects of early detection, high sensitivity and specificity, and safe sampling
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Embodiment 1
[0035] Example 1 Screening of peripheral blood characteristic gene markers of breast cancer
[0036] The screening of peripheral blood characteristic genes of breast cancer includes the following steps:
[0037] 1) Using PAXgene TM The Blood RNA Tube blood collection tube collects peripheral blood samples from 55 cases of breast cancer patients confirmed by pathological examination and 50 cases of normal people, and each sample collects 2-3mL peripheral blood.
[0038] 2) The total RNA of the above peripheral blood samples was extracted using the PAXgene Blood RNA Kit extraction kit. Agilent Bioanalyzer 2100 bioanalyzer was used to detect the fragment integrity (RIN) of RNA samples, and the purity of RNA samples was detected by Nano1000 micro-ultraviolet spectrophotometer. All RNA samples must meet the following quality control conditions: RNA yield greater than 2 micrograms, 28S / 18S peak ratio greater than 1, RIN value greater than 7, and 260nm / 280nm absorbance ratio greate...
Embodiment 2
[0050] Example 2 Detection of Breast Cancer Using Screened Breast Cancer Peripheral Blood Characteristic Gene Markers
[0051] 1. Method steps:
[0052] 1) Collection of peripheral blood samples of samples to be tested: using PAXgene TM Blood RNA Tube blood collection tube collects 2ml of peripheral blood from the subject's cubital vein;
[0053] 2) Extraction and purification of total RNA in the peripheral blood samples of the samples to be tested: use the PAXgene Blood RNA Kit extraction kit to extract and purify the total RNA in the peripheral blood, and use the Agilent BioAnalyzer 2100 microelectrophoresis analyzer to identify the integrity of the extracted total RNA fragments and yield, the purity of the RNA sample was detected with a Nano1000 micro-volume ultraviolet spectrophotometer;
[0054] 3) Reverse transcription reaction: use Life Techonolgy's High-Capacity cDNA ReverseTranscription kit reverse transcription kit, use total RNA as a template, and use Olig(dT) as ...
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